Development of high resolution imaging for tumor boundary delineation using 7-tesla magnetic resonance imaging enhanced by high iron diamine immersion

• Project/Area Number: 24659283
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Laboratory medicine
• Research Institution: Iwate Medical University
• Principal Investigator: MAESAWA Chihaya 岩手医科大学, 医学部, 教授 (10326647)
• Co-Investigator(Kenkyū-buntansha): 千葉 俊美 岩手医科大学, 医学部, 准教授 (40254784) ; 佐々木 真理 岩手医科大学, 医学部, 教授 (80205864)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: MRI / 病理 / 高鉄ジアミン染色 / EMR / ESD / 臨床病理 / LST / 大腸癌 / 臨床病理学

Research Abstract

We developed a method for ex vivo imaging using 7-tesla high-resolution magnetic resonance imaging (MRI) combined with high-iron diamine (HID) immersion.All MR images were acquired with a 7-tesla scanner by spoiled gradient recalled acquisition using the steady state (SPGR) gradient-echo sequence. Colorectal specimens obtained from normal rats and model rats with colon cancer induced by dimethylhydrazine (DMH) were enhanced by immersion in colloidal iron or HID as contrast agents for goblet cell mucin, and subsequently examined by 7-tesla MRI.Although colloidal iron immersion failed to yield well contrasted images of the colonic epithelium, the HID immersion method clearly delineated the tumor boundary. Contrast ratios differed significantly between cancerous tissue and normal epithelium (P<0.01). Our established ex vivo 7-tesla MRI method combined with HID immersion is a unique approach for delineating the tumor boundary in ESD/EMR specimens.

Research Products

• [Journal Article] Immunohistochemistry for histone h3 lysine 9 methyltransferase and demethylase proteins in human melanomas. (2014)
  • Author(s): Miura S, Maesawa C, Shibazaki M, Yasuhira S, Kasai S, Tsunoda K, Maeda F, Takahashi K, Akasaka T, Masuda T. Immunohistochemistry for histone h3 lysine 9 methyltransferase and demethylase proteins in human melanomas.
  • Journal Title: Am J Dermatopathol Volume: 36(3) Issue: 3 Pages: 211-216
  • DOI: 10.1097/dad.0b013e3182964e02
  • Peer Reviewed / Open Access
• [Journal Article] A disintegrin and metalloproteinase 17 (ADAM17) mediates epidermal growth factor receptor transactivation by angiotensin II on hepatic stellate cells. (2014)
  • Author(s): Oikawa H, Maesawa C, Tatemichi Y, Nishinari Y, Nishiya M, Mizugai H, Ikeda A, Oikawa K, Takikawa Y, Masuda T.
  • Journal Title: Life Sci. Volume: 97(2) Issue: 2 Pages: 137-144
  • DOI: 10.1016/j.lfs.2013.12.028
  • Peer Reviewed
• [Journal Article] A somatic mutation of the KEAP1 gene in malignant melanoma is involved in aberrant NRF2 activation and an increase in intrinsic drug resistance. (2014)
  • Author(s): Miura S, Shibazaki M, Kasai S, Yasuhira S, Watanabe A, Inoue T, Kageshita Y, Tsunoda K, Takahashi K, Akasaka T, Masuda T, Maesawa C.
  • Journal Title: J Invest Dermatol. Volume: 134(2) Issue: 2 Pages: 553-556
  • DOI: 10.1038/jid.2013.343
  • Peer Reviewed
• [Journal Article] BCL2 and BCLxL are key determinants of resistance to antitubulin chemotherapeutics in melanoma cells. (2013)
  • Author(s): Watanabe A, Yasuhira S, Inoue T, Kasai S, Shibazaki M, Takahashi K, Akasaka T, Masuda T, Maesawa C.
  • Journal Title: Exp Dermatol Volume: 22(8) Issue: 8 Pages: 518-523
  • DOI: 10.1111/exd.12185
  • Peer Reviewed
• [Journal Article] Subcutaneous panniculitis-like T-cell lymphoma (SPTCL) with hemophagocytosis (HPS): successful treatment using high-dose chemotherapy (BFM-NHL & ALL-90) and autologous peripheral blood stem cell transplantation. (2013)
  • Author(s): Sakurai E, Satoh T, Akiko YA, Maesawa C, Tsunoda K, Endo M, Akasaka T, Masuda T.
  • Journal Title: J Clin Exp Hematop Volume: 53(2) Pages: 135-140
  • Peer Reviewed
• [Journal Article] Loss of HOXD10 expression induced by upregulation of miR -10b accelerates themigration and invasion activities of ovarian cancer cells (2013)
  • Author(s): Nakayama I, Shibazaki M, Yashima-Abo A, Miura F, Sugiyama T, Masuda T, MaesawaC
  • Journal Title: Int J Oncol Volume: 43(1) Issue: 1 Pages: 63-71
  • DOI: 10.3892/ijo.2013.1935
  • Peer Reviewed
• [Journal Article] Overexpre ssion of histone deacetylase 6 contributes to accelerated migration and invasion activity of hepatocellular carcinoma cells. (2012)
  • Author(s): Kanno K, Kanno S, Nitta H, Uesugi N, Sugai T, Masuda T, Wakabayashi G , Maesawa C
  • Journal Title: Oncol Rep. Volume: 28(3) Issue: 3 Pages: 867-73
  • DOI: 10.3892/or.2012.1898
  • Peer Reviewed
• [Journal Article] Molecular marker identification for relapse prediction in 5-FU-based adjuvant chemotherapy in gastric and colorectal cancers. (2012)
  • Author(s): Ishida K, Nishiduka S, Chiba T, Ikeda M, Kume K, Endo F, Katagiri H, Matsuo T, Noda H, Iwaya T, Yamada N, Fujiwara H, Takahashi M, Itabashi T, Uesugi N,Maesawa C, Tamura G, Sugai T, Otsuka K, Koeda K, Wakabayashi G.
  • Journal Title: PLoS One Volume: 7(8) Issue: 8 Pages: e43236-e43236
  • DOI: 10.1371/journal.pone.0043236
  • Peer Reviewed