| Project/Area Number |
24659299
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hygiene
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| Research Institution | Chubu University |
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Principal Investigator |
NASU Tamie 中部大学, 生命健康科学部, 教授 (10020794)
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| Co-Investigator(Kenkyū-buntansha) |
NAITO Hisao 藤田保健衛生大学, 医学部, 講師 (90547556)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | トリクロロエチレン / Hypersensitivity / 肝障害 / 全身性皮膚炎 / CYP2E1 / GPMT / ALT / IFNγ / CYP2E1抗体 / トリクロロエタノール / HK293細胞 / NIH3T3細胞 / CYP2E1cDNA / 患者血清 / トリクロロ酢酸 / モルモット / 免疫沈降法 / 感作性 / サイトカイン / プロテオミクス |
|---|
| Outline of Final Research Achievements |
Trichloroethylene (TRI), trichloroacetic acid and trichloroethanol (TCE) maximization tests using guinea pigs (GPMT) were done. Sensitization to TRI was observed in males and females, whereas that to TCE was observed only in females. In guinea pigs with sensitization, focal necrosis with inflammatory cell infiltrations was observed in the livers at irregular intervals. In addition, serum ALT and IFNγ levels were higher in sensitized animals than those without sensitization. These findings were obviously observed in animals exposed to TRI. In serum of guinea pigs with sensitization, proteins having 25 and 50kD to react with anti-CYP2E1 were increased in animals with sensitization. Proteomics analysis showed that these proteins may be IgG. As a preliminary experiment, culture cells contained human CYP2E1 reacted with serum of patients with hypersensitivity and control workers. The reacted IgG to CYP2E1 appeared to be greater in the serum of patients than controls.
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