Functional genetic screening of the genes involved in the arsenite sensitivity
| Project/Area Number |
24659301
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Hygiene
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 三酸化ヒ素 / 薬毒物感受性 / 薬毒物耐性 / 抗がん剤耐性 / ジーントラップ / ゲノム機能 / トキシコゲノミクス / 責任遺伝子探索 / ヒ素 / 責任遺伝子 / P450 / 活性酸素 |
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| Outline of Final Research Achievements |
To clarify the mechanisms underlying the toxicity of arsenite, we conducted a functional genetic screening of the genes involved in the sensitivity to arsenite using the gene trap insertion mutant cells library. From the analysis of 34 mutants showed decreased sensitivity to arsenite, eleven sensitivity-related candidate genes (one P450-related gene, one signaling-related genes, two protein synthesis-related genes, one cancer-related gene, six unidentified function genes) were identified. We also showed that the P450 enzyme system and Wnt / catenin pathway are the determining factors of sensitivity to arsenite.
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Report
(5 results)
Research Products
(14 results)
