A metabolomics study of children with neurodevelopmental problems due to dioxin exposure

• Project/Area Number: 24659306
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Hygiene
• Research Institution: Kanazawa Medical University
• Principal Investigator: NAKAGAWA Hideaki 金沢医科大学, 医学部, 教授 (00097437)
• Co-Investigator(Kenkyū-buntansha): NISHIJO Muneko 金沢医科大学, 医学部, 准教授 (40198461)
• Co-Investigator(Renkei-kenkyūsha): MORIKAWA Yuko 金沢医科大学, 看護学部, 教授 (20210156) ; NAKAMURA Kousi 金沢医科大学, 医学部, 准教授 (80422898) ; SAKURAI Masaru 金沢医科大学, 医学部, 准教授 (90397216)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ダイオキシン / メタボロミクス / 尿検査 / 脳神経発達 / 自閉症スペクトラム障害 / 早期指標 / ベトナム / 社会情動発達 / 小児

Research Abstract

In a hot spot of dioxin contamination in Vietnam, we examined social emotional behaviors by autism spectrum rating scale (ASRS) of 3-year-old children and found that the high TCDD exposed group showed higher ASRS scores as compared with the lower exposed groups, suggesting autistic traits. Then, we analyzed urinary metabolomes of the high TCDD group and age and gender matched low exposure groups and detected candidate compound to discriminate these high and low TCDD groups. Urinary histidine and tryptophan were selected for good negative markers and significant decreased levels of these amino acids were observed in the high TCDD group. However, no significant correlation was observed between dioxin levels in breast milk and urinary levels of histidine and tryptophan in 68 6-month-old infants living in another hot spot in Vietnam. They might be too young to detect alteration of urinary amino acids. We need to follow-up them for longer time and collect more number of urine specimens.

Research Products

• [Presentation] ベトナムの高汚染地域に居住する3歳児におけるダイオキシン暴露と自閉症傾向 (2014)
  • Author(s): 西条旨子、Tai PT, Anh NTN, Tran Ngoc Nghi中川秀昭、城戸照彦、西条寿夫
  • Organizer: 第84回日本衛生学会総会
  • Place of Presentation: 岡山コンベンションセンター(岡山市)
  • Year and Date: 2014-05-27
• [Presentation] High TCDD levels in breast milk of mothers living in a dioxin hot spot in Bien Hoa city, Vietnam (2014)
  • Author(s): Nghi NN, Manh HD, Tai PT, Nishijo M, Anh NTN, Maruzeni S, Nakagawa H, Kido T, Nishijo H.
  • Organizer: 第84回日本衛生学会総会
  • Place of Presentation: 岡山コンベンションセンター(岡山市)
  • Year and Date: 2014-05-27
• [Presentation] Perinatal dioxin exposure and neurodevelopment of children aged 3 years–A follow up study in Vietnam (2013)
  • Author(s): Tai PT, Nishijo M, Anh NTN, Maruzeni S, Nakagawa H, Luong HV, Anh TH, Nhat TV, Thanh TT, Phuong PT, Son LK, Morikawa Y, Kido T, Nishijo H.
  • Organizer: 33rd international Symposium on Halogenated Persistent Organic pollutants
  • Place of Presentation: 大邱(韓国)
  • Year and Date: 2013-08-29
• [Presentation] Dioxin concentration in breast milk of primipara mothers in Bien Hoa, a hot spot area in Vietnam-A preliminary study in 2012- (2013)
  • Author(s): Nishijo M, Nghi TN, Manh HD, Tai PT, Anh NTN, Vu PHA, Nguyen NT, Hung NX, Hoan HM, Hai HC, Nishijo H, Hai-Anh T, Luong HV, Kido T, Okamoto R, Son LK, Nakagawa H.
  • Organizer: 33rd international Symposium on Halogenated Persistent Organic pollutants
  • Place of Presentation: 大邱(韓国)
  • Year and Date: 2013-08-27
• [Presentation] Relationships between food intake and dioxin concentrations in breast milk of mothers living in a hot spot of dioxin contamination in Vietnam (2013)
  • Author(s): Nguyen Thi Nguyet Anh, Pham The Tai, Nishijo M, Maruzeni S, Anh TH, Dam N, Luong HV, Morikawa Y, Nakagawa H, Nishijo H.
  • Organizer: 第83回日本衛生学会総会
  • Place of Presentation: 金沢市
  • Year and Date: 2013-03-26
• [Presentation] Effects of perinatal exposure to dioxins on neurodevelopment in Vietnamese children at 3 years of age (2013)
  • Author(s): Tran Ngoc Nghi, Pham The Tai, Nguyen Thi Nguyet Anh, Maruzeni S, Nishijo M, Honda R, Nishijo H, Morikawa Y, Nakagawa H.
  • Organizer: 第83回日本衛生学会総会
  • Place of Presentation: 金沢市
  • Year and Date: 2013-03-26
• [Presentation] ダイオキシン環境曝露の出生児の脳神経発達に与える影響 : ベトナムにおける小児コホート3年間の追跡 (2013)
  • Author(s): 西条旨子、Pham The Tai, Nguyen Thi Nguyet Anh, Tran Ngoc Nghi,丸銭笑子,中川秀昭,森河裕子,岡本理恵,城戸照彦, Hoang Van Luong, Tran Hai Anh, Phan Thanh Phuong,西条寿夫
  • Organizer: 第23回日本疫学会学術総会
  • Place of Presentation: 吹田市
  • Year and Date: 2013-01-26
• [Presentation] Dioxin concentration in breast milk of primipara mothers in Bien Hoa, a hot spot area in Vietnam － A preliminary study in 2012－ (2013)
  • Author(s): Nishijo M, Nghi TN, Manh HD, Tai PT, Anh NTN, Vu PHA, Nguyen NT, Hung NX, Hoan HM, Hai HC, Nishijo H, Hai-Anh T, Luong HV, Kido T, Okamoto R, Son LK, Nakagawa H
  • Organizer: The 33rd international Symposium on Halogenated Persistent Organic pollutants - Dioxin 2013
  • Place of Presentation: Hotel Inter-Burgo, Daegue, Korea
• [Presentation] Perinatal dioxin exposure and neurodevelopment of children aged 3 years – A follow up study in Vietnam (2013)
  • Author(s): Tai PT, Nishijo M, Anh NTN, Maruzeni S, Nakagawa H, Luong HV, Anh TH, Nhat TV, Thanh TT, Phuong PT, Son LK, Morikawa Y, Kido T, Nishijo H
  • Organizer: The 33rd international Symposium on Halogenated Persistent Organic pollutants - Dioxin 2013
  • Place of Presentation: Hotel Inter Burgo, Daegue, Korea
• [Presentation] Effects of perinatal exposure to dioxins on neurodevelopment in Vietnamese children at 3 years of age. (2013)
  • Author(s): Tran Ngoc Nghi
  • Organizer: 第83回日本衛生学会総会
  • Place of Presentation: 金沢大学保健学科（金沢市）