Isolation of Campylobacter phage from poultry and reduction of food-borne outbreak by Campylobacter jejuni/coli
Project/Area Number |
24659307
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Single-year Grants |
Research Field |
Hygiene
|
Research Institution | Kinki University |
Principal Investigator |
ISHII Eiji 近畿大学, 生物理工学部, 教授 (80158740)
|
Project Period (FY) |
2012-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
|
Keywords | カンピロバクター / 食中毒 / 鶏肉 / ファージ分離法 / 生物製剤 |
Research Abstract |
A total of 100 Campylobacter jejuni/coli strains was isolated from 223 specimens of poultry liver purchased at 6 retail stores. Three crude phages were detected from the Preston enrichment culture of livers and divided into seven pure phages, which made the plaque against 30 C.jejuni/coli isolates. The phage suspension was obtained at more than one billion pfu/ml, and the plaque forming activity of these phages in the SM buffer solution was retained for more than 15 months after preservation at 4C, -20C, and -80C.
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Report
(3 results)
Research Products
(4 results)