Identification of novel enterotoxin of Clostridium perfringens produced by human clinical isolates from outbreaks of acute gastroenteritis and application of diagnosis

• Project/Area Number: 24659310
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Hygiene
• Research Institution: Osaka Prefectural Institute of Public Health
• Principal Investigator: YONOGI Shinya 大阪府立公衆衛生研究所, その他部局等, 研究員 (20553613)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: ウェルシュ菌 / 食中毒 / エンテロトキシン / 新型エンテロトキシン / BEC / 食品衛生

Research Abstract

Clostridium perfringens is a causative agent of food-borne gastroenteritis which for C. perfringens Enterotoxin (CPE) produced in intestinal tract has been considered an essential factor. We experienced two outbreaks of food-borne gastroenteritis in which non-CPE producers of C. perfringens were strongly suspected to be the cause and analyzed the clinical isolates. In this study, we identified a novel enterotoxin and named BEC (binary enterotoxin of C. perfringens). In addition, we developed detection method for becAB gene and applied to routine test of food-borne gastroenteritis.

Research Products

• [Journal Article] BEC, a novel enterotoxin of Clostridium perfringens found in human clinical isolates from acute gastroenteritis outbreaks (2014)
  • Author(s): Yonogi S, Matsuda S, Kawai T, Yoda T, Harada T, Kumeda Y, Gotoh K, Hiyoshi H, Nakamura S, Kodama T, Iida T
  • Journal Title: Infect Immun. Volume: [Epub ahead of print]
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/24664508
  • Peer Reviewed
• [Journal Article] BEC, a novel enterotoxin of Clostridium perfringens found in human clinical isolates from acute gastroenteritis outbreaks (2014)
  • Author(s): Shinya Yonogi, Shigeaki Matsuda, Takao Kawai, Tomoko Yoda, Tetsuya Harad, Yuko Kumeda, Kazuyoshi Gotoh, Hirotaka Hiyoshi, Shota Nakamura, Toshio Kodama, Tetsuya Iida
  • Journal Title: Infection and Immunity Volume: 82 Issue: 6 Pages: 999-999
  • DOI: 10.1128/iai.01759-14
  • Peer Reviewed / Open Access