Development of a new method of age estimation-an approach from transcription factors-

• Project/Area Number: 24659335
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Legal medicine
• Research Institution: University of Fukui
• Principal Investigator: IIDA Reiko 福井大学, 医学部, 准教授 (40139788)
• Co-Investigator(Renkei-kenkyūsha): UEKI Misuzu 福井大学, 医学部, 助手 (00165656)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 法医学 / 年齢推定 / 転写因子

Outline of Final Research Achievements

I have identified a number of genes which were up- or down-regulated by siRNA-mediated knockdown of M-LPH or Rhit. No significant differences in histological appearance were observed between Rhit-/Rhit- mice and wild-mice. It was shown that RhitH binds to the Ttk 69K binding site and functions as a transcriptional repressor in M-LPH expression, and that expression of RhitH gene is transcriptionally regulated by two transcription factors, FOXD3 and GABP.

Research Products

• [Journal Article] Evaluation of all non-synonymous single nucleotide polymorphisms (SNPs) in the genes encoding human deoxyribonuclease I and I-like 3 as a functional SNP potentially implicated in autoimmunity (2014)
  • Author(s): Misuzu Ueki
  • Journal Title: FEBS Journal Volume: 281 Issue: 1 Pages: 376-390
  • DOI: 10.1111/febs.12608
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Three non-synonymous single nucleotide polymorphisms in the RhitH gene cause reduction of the repression activity that leads to up-regulation of M-LPH, a participant in mitochondrial function (2013)
  • Author(s): R. Iida, M. Ueki, J.Fujiwara, H. Takeshita, K. Kimura-Kataoka, T. Yasuda
  • Journal Title: BioResearch Open Access Volume: 2 Issue: 6 Pages: 440-447
  • DOI: 10.1089/biores.2013.0042
  • Peer Reviewed
• [Journal Article] Identification of Rhit as a novel transcriptional repressor of human Mpv17-like protein with a mitigating effect on mitochondrial dysfunction, and its transcriptional regulation by FOX D3 and GABP (2012)
  • Author(s): R.Iida, et al
  • Journal Title: Free Radical Biology and Medicine Volume: 52 Issue: 8 Pages: 1413-1422
  • DOI: 10.1016/j.freeradbiomed.2012.01.003
  • Peer Reviewed
• [Presentation] 転写因子RhitH遺伝子における非同義置換型SNPの遺伝的解析と転写抑制に及ぼす効果 (2013)
  • Author(s): 飯田 礼子, 安田 年博, 竹下 治男
  • Organizer: 第86回日本生化学会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
• [Presentation] ヒト年齢依存性転写抑制因子RhitHの同定とその転写調節機構 (2013)
  • Author(s): 安田 年博, 飯田 礼子, 竹下 治男, 小湊 慶彦
  • Organizer: 第97次日本法医学会学術全国集会
  • Place of Presentation: ロイトン札幌（札幌市）
• [Presentation] Identification of a novel transcriptional repressor, RhitH, involved in expression of human Mpv17-like protein, and its transcriptional regulation by FOXD3 and GABP (2012)
  • Author(s): R.Iida
  • Organizer: 日本生化学会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡（福岡県）