Application of bile acids as biomarkers for forensic medicine
Project/Area Number |
24659337
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Legal medicine
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Research Institution | Nagoya University |
Principal Investigator |
Ishii Akira 名古屋大学, 医学(系)研究科(研究院), 教授 (30252175)
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Co-Investigator(Kenkyū-buntansha) |
HATTORI HIDEKI 名古屋大学, 医学部, 招へい教員 (30107817)
OGAWA TADASHI 愛知医科大学, 医学部, 講師 (50559937)
ZAITSU KEI 名古屋大学, 大学院医学系研究科, 准教授 (30700546)
ASANO TOMOMI 金城学院大学, 生活環境学部, 助教 (90637951)
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Project Period (FY) |
2012-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | 胆汁酸 / タンデム質量分析 / 非アルコール性肝障害 / ナノLC / 抱合体 / 質量分析 / タンデム質量分析計 / ナノスケールLC / 高速液体クロマトグラフィー / 固相抽出 / 超高速液体クロマトグラフ / SHRSPラット |
Outline of Final Research Achievements |
Bile acids are steroid derivatives in bile that consist of various isomers and their conjugates; bile acid profiles are associated with different pathological states. We have developed comprehensive methods for measuring bile acids in biological fluids, and have applied them to the analyses of various hepatic diseases in pathology as well as in forensic medicine. We established a rapid quantitation method for 21 bile acids in serum and liver samples by ultra-performance liquid chromatography-tandem mass spectrometry, achieving complete separation within 5 minutes. Having validated the method, we measured bile acids in the samples of model rats fed with high-fat and high-cholesterol-containing diet, and observed the elevation of glycine conjugates of several bile acids and total cholic and chenodeoxycholic acids. In addition, we also developed a highly sensitive detection method for 28 bile acids using a nano-scale LC-MS-MS.
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Report
(5 results)
Research Products
(64 results)
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[Journal Article] Identification of N,N-bis(1-pentylindol-3-yl-carboxy)naphthylamine (BiPICANA) found in an herbal blend product in the Tokyo metropolitan area and its cannabimimetic effects evaluated by in vitro [35S]GTPγS binding assays2015
Author(s)
J Nakajima, M Takahashi, N uemura, T Seto, H Fukaya, J Suzuki, M Yoshida, M Kusano, H Nakajima, K Zairau, A Ishii, T Moriyasu, D Nakae
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Journal Title
Forensic Toxicology
Volume: 33
Issue: 1
Pages: 84-92
DOI
Related Report
Peer Reviewed
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[Journal Article] A Possible role of chenodeoxycholic acid and glycine-conjugated bile acids in fibrotic steatohepatitis in a dietary rat model2014
Author(s)
Jia X, Suzuki Y, Naito H, Yetti H, Kitamori K, Hayashi Y, Kaneko R, Nomura M, Yamori Y, Zaitsu K, Kato M, Ishii A, Nakajima T
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Journal Title
Dig Dis Sci
Volume: -
Issue: 7
Pages: 1490-1501
DOI
Related Report
Peer Reviewed
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[Journal Article] Metabolism of the newly encountered designer drug α-pyrrolidinovalerophenone in humans: identification and quantitation of urinary metabolites.2014
Author(s)
Noriaki Shima, Munehiro Katagi, Hiroe Kamata, Shuntaro Matsuta, Keiko Sasaki, Tohru Kamata, Hiroshi Nishioka, Akihiro Miki, Michiaki Tatsuno, Kei Zaitsu, Akira Ishii, Takako Sato, Hitoshi Tsuchihashi, Koichi Suzuki
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Journal Title
Forensic Toxicology
Volume: 32
Issue: 1
Pages: 59-67
DOI
Related Report
Peer Reviewed
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[Journal Article] Development of a simple one-pot extraction method for various drugs and metabolites of forensic interest in blood by modifying the QuEChERS method2013
Author(s)
Shuntaro Matsuta, Keiko Nakanishi, Akihiro Miki, Kei Zaitsu, Noriaki Shima, Tooru Kamata, Hiroshi Nishioka, Munehiro Katagi, Michiaki Tatsuno, Kento Tsuboi, Hitoshi Tsuchihashi, Koichi Suzuki
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Journal Title
Forensic Science International
Volume: 232
Issue: 1-3
Pages: 40-45
DOI
Related Report
Peer Reviewed
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[Journal Article] Mass spectrometric differentiation of the isomers of mono-methoxyethylamphetamines and mono-methoxydimethylamphetamines by GC/SM/MS2013
Author(s)
Kei Zaitsu, Haruhiko Miyagawa, Yuki Sakamoto, Shuntaro Matsuta, Kento Tsuboi, Hiroshi Nishioka, Munehiro Katagi, Takako Sato, Michiaki Tatsuno, Hitoshi Tsuchihashi, Koichi Suzuki, Akira Ishii.
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Journal Title
Forensic Toxicology
Volume: 31
Issue: 2
Pages: 292-300
DOI
Related Report
Peer Reviewed
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[Journal Article] Urinary excretion and metabolism of the newly encountered designer drug 3,4-dimethylmethcathinone in humans2013
Author(s)
Noriaki Shima, Munehiro Katagi, Hiroe Kamata, Shuntaro Matsuta, Keiko Nakanishi, Kei Zaitsu, Tooru Kamata, Hiroshi Nishioka, Akihiro Miki, Michiaki Tatsuno, Takako Sato, Hitoshi. Tsuchihashi, Koichi Suzuki
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Journal Title
Forensic Toxicology
Volume: 31
Issue: 1
Pages: 101-112
DOI
Related Report
Peer Reviewed
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[Presentation] 心房内に多発性血管腫を認めた一例2015
Author(s)
石井 晃, 吉本高士, 山本敏充, 財津 桂, 草野麻衣子, 伊藤雅文
Organizer
第37回日本法医学会学術中部地方集会
Place of Presentation
山梨県立図書館、山梨県、甲府市
Year and Date
2015-10-16
Related Report
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[Presentation] GC/MS/MSによる合成カンナビノイドJWH-081およびその位置異性体の識別2014
Author(s)
草野麻衣子, 財津 桂, 中山 浩, 中嶋順一, 鈴木 仁, 守安貴子, 坪井健人, 松田駿太朗, 片木宗弘, 土橋 均, 石井 晃
Organizer
日本法中毒学会第33年会
Place of Presentation
名古屋大学シンポジオンホール、愛知県、名古屋市
Year and Date
2014-07-05
Related Report
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[Presentation] Y-STR haplotype network analysis in Mongolian Population2014
Author(s)
T Yamamoto, Y Hiroshige, H Ogawa, T Senda, D Horiba, M Sakuma, Y Kawaguchi, Y Kano, K Zaitsu, T Yoshimoto, A Ishii
Organizer
The 9 th International Symposium on Advances in Legal Medicine (ISALM)
Place of Presentation
福岡国際会議場、福岡県、福岡市
Year and Date
2014-06-17
Related Report
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[Presentation] Development of a forensic toxicological database of natural toxins by LC/Q-TOFMS2014
Author(s)
K Zaitsu, T Ogawa, T Kokaji, T Suga, M Kusano, Y Shiraishi, H Hattori, T Sato, H Tsuchihashi, H Seno, A Ishii
Organizer
The 9 th International Symposium on Advances in Legal Medicine (ISALM)
Place of Presentation
福岡国際会議場、福岡県、福岡市
Year and Date
2014-06-17
Related Report
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[Presentation] High-resolution mass spectrometry and accurate mass measurement of synthetic cannabinoids and their metabolites in human biological specimens by LC/Q-TOFMS2013
Author(s)
Kei Zaitsu, Takashi Yoshimoto, Toshimichi Yamamoto, Tadashi Ogawa, Hideki Hattori, Tomohiro Matsumoto, Kazuaki Hisatsune, Munehiro Katagi, Hitoshi Tsuchihashi, Akira Ishii.
Organizer
The 51 st Annual Meeting of the International Association of Forensic Toxicologists
Place of Presentation
Funchal, Portugal
Related Report
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[Presentation] GC-MS/MSを用いたナフトイルインドール系薬物の包括的検出2013
Author(s)
宮川 治彦, 坂本 雄紀, 松田 駿太朗, 鎌田 徹, 西岡 裕, 片木 宗弘, 財津 桂, 石井 晃, 坪井 健人, 土橋 均, 鈴木 廣一
Organizer
日本法中毒学会第32年会
Place of Presentation
さわやかちば県民プラザ、柏
Related Report
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