Development of a reliable semiquantitative screening method for forensic samples
| Project/Area Number |
24659338
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Legal medicine
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| Research Institution | Kyushu University |
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Principal Investigator |
KUDO KDIKO 九州大学, 医学(系)研究科(研究院), 講師 (10186405)
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| Co-Investigator(Kenkyū-buntansha) |
HAYASHIDA Makiko 日本医科大学, 医学部, 准教授 (60164977)
SAKA Kanju 東京大学, 医学系研究科 (30447388)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 法医中毒学 / 薬毒物スクリーニング / NAGINATA / GC-MS / LC-MS/MS / QuEChERS法 |
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| Research Abstract |
We have developed a rapid procedure for simultaneous extraction of a wide range of acidic and basic drugs from whole blood samples for reliable semi-quantitative drug screening by GC-MS. To extract the wide range of drugs, the partition/extraction procedure used for the QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) method was selected at the initial step and the protein-lipid removal filter cartridge (Captiva ND Lipids) was found to be useful to remove interference compounds to obtain clean extracts as the second step to be connected to GC-MS screening using the NAGINATA software with our constructed calibration-locking database. We also examined the applicability of our established method in an actual forensic case. The established preparation procedure was applied to tissue samples with some modification, and it was also suitable for LC-MS/MS screening.
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Report
(3 results)
Research Products
(22 results)
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[Presentation] Rapid screening and semi-quantitative analysis for forensic drugs in blood using liquid chromatography triple quadrupole mass spectrometry2013
Author(s)
H. Thomas, K. Kudo, T. Minohata, K.. Usui, N. Shima, M. Katagi , H. Tsuchihashi, K. Suzuki, I. Hirano, N Ikeda
Organizer
61th ASMS Conference on Mass Spectrometry; Abstracts
Place of Presentation
Minneapolis, Minnesota, USA
Related Report
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[Presentation] Screening analysis for drugs of abuse by LC-MS/MS enables fast polarity switching MRM triggered product ion scanning on the fly. 2012 p1162012
Author(s)
T Minohata, I Hirano, J Iida, K Kudo, N Ikeda, K Zaitsu, N Shima, M Katagi, H Tsuchihashi, K Suzuki
Organizer
19th International Mass Spectrometry Conference
Place of Presentation
Kyoto,Japan
Related Report
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