Development of novel diagnostic methods for anaphylactic shock based on gene expression
| Project/Area Number |
24659339
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Legal medicine
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
KIMURA Akihiko 和歌山県立医科大学, 医学部, 准教授 (60136611)
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| Co-Investigator(Kenkyū-buntansha) |
NOSAKA Mizuho 和歌山県立医科大学, 医学部, 助教 (00244731)
ISHIDA Yuko 和歌山県立医科大学, 医学部, 講師 (10364077)
KONO Toshikazu 和歌山県立医科大学, 医学部, 教授 (70251923)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | アナフィラキシーショック / 法医診断 / 急性死 / ショック / アナフィラキシー |
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| Research Abstract |
Specifying an anaphylactic shock as the cause of death only based on the autopsy findings often encounters difficulties. So, in this research, we tried development of the novel diagnostic method for anaphylactic shock using the mouse model. Anaphylactic shock was induced by challenge of penicillin V as an antigen, and total RNA was extracted from the various organs of mice died. The extracted RNA was subjected to real-time RT-PCR for analyses of the gene expression of cytokines, chemokines, the molecules for immune regulation, growth factors, transcription factors and signal transducers to identify the changes in specific gene expression anaphylactic shock. However, at present, we did not find the changes of gene expression specific for anaphylactic shock.
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Report
(3 results)
Research Products
(15 results)
