Development of drugs targeting hepatitis B x-protein
| Project/Area Number |
24659359
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Gastroenterology
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
SAKAMOTO NAOYA 北海道大学, 医学(系)研究科(研究院), 教授 (10334418)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NAKANISHI Mitsuru 北海道大学, 医学研究科, 特任助教 (10392250)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | ウイルス / 医学 / ウイルス学 / 基礎研究 / B型肝炎 |
|---|
| Research Abstract |
We have conducted study project to develop new classes of antiviral drug against hepatitis B virus (HBV). In the study period, we obtained the following results. (1) HBV expression plasmid which selectively lacks HBX protein showed decreased expression levels as compared with wild type when transfected into humans hepatoma Huh7 cells. (2) Mutant HBV expression plasmids were constructed in which 4 consecutive alanine substitution were introduced in the HBX protein coding frame. By using the mutant HBX plasmids, we found AP1 transactivator domain in C-terminal of HBX.
|
Report
(3 results)
Research Products
(40 results)
-
-
[Journal Article] Heat shock factor 1 accelerates hepatocellular carcinoma development by activating nuclear factor κB/mitogen-activated protein kinase.2014
Author(s)
Chuma M, Sakamoto N, Nakai A, Hige S, Nakanishi M, Natsuizaka M, Suda G, Sho T, Hatakaka K, Matsuno Y, Yokoo H, Kamiyama T, Taketomi A, Fujii G, Tashiro K, Hikiba Y, Fujimoto M, Asaka M, Maeda S
-
Journal Title
Carcinogenesis
Volume: Epub ahead of print
Issue: 2
Pages: 272-281
DOI
Related Report
Peer Reviewed
-
-
-
[Journal Article] Hypoxia-inducible Factors Activate CD133 Promoter through ETS Family Transcription Factors2013
Author(s)
Ohnishi S, Maehara O, Nakagawa K, Kameya A, Otaki K, Fujita H, Higashi R, Takagi K, Asaka M, Sakamoto N, Kobayashi M, Takeda H
-
Journal Title
Plos One
Volume: 8
Issue: 6
Pages: e66255-e66255
DOI
NAID
Related Report
Peer Reviewed
-
[Journal Article] Wnt5a Signaling Mediates Biliary Differentiation of Fetal Hepatic Stem/Progenitor Cells2013
Author(s)
Kakinuma S, Kiyohashi K, Kamiya A, Sakamoto N, Nitta S, Yamanaka H, YoshinoK, Fujiki J, Murakawa M, Kusano-Kitazume A, Shimizu H, Okamoto R, Azuma S, Nakagawa M, Asahina Y, Tanimizu N, Kikuchi A, Nakauchi H, and *WatanabeM
-
Journal Title
Hepatology
Volume: (in press)
Issue: 6
Pages: 2502-2513
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
[Journal Article] Hepatitis C virus NS4B protein targets STING and abrogates RIG-I-mediated type-I interferon-dependent innate immunity2013
Author(s)
Nitta S, Sakamoto N, Nakagawa M, Kakinuma S, Mishima K, Kusano-kitazume A, Kiyohashi K, Murakawa M, Nishimura-Sakurai Y, Azuma S, Tasaka-Fujita M, asahina Y, Yoneyama M, Fujita T, Watanabe M
-
Journal Title
Hepatology
Volume: 57
Issue: 1
Pages: 46-58
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] Factors responsible for the discrepancy between IL28B polymorphism prediction and the viral response to peginterferon plus ribavirin therapy in Japanese chronic hepatitis C patients2012
Author(s)
Saito H, Ito K, Sugiyama M, Matsui T, Aoki Y, Imamura M, Murata K, Masaki N, Nomura H, Adachi H, Hige S, Enomoto N, Sakamoto N, Kurosaki M, Mizokami M, Watanabe S
-
Journal Title
Hepatol Res
Volume: 42(10)
Issue: 10
Pages: 958-65
DOI
Related Report
Peer Reviewed
-
-
-
-
-
[Journal Article] Ochanomizu Liver Conference Study Group : Association of ITPA gene variant and serum ribavirin concentration with blood cells decline in pegylated interferon-alfa plus ribavirin therapy for chronic hepatitis C2012
Author(s)
Nakagawa M, Sakamoto N, Watanabe T, Nishimura-Sakurai Y, Onozuka I, Azuma S, Kakinuma S, Nitta S, Kiyohashi K, Kusano-Kitazume A, Murakawa M, Yoshino K, Itsui Y, Tanaka Y, Mizokami M, Watanabe M.
-
Journal Title
Hepatol Int
Volume: (Epub ahead of print)
Related Report
Peer Reviewed
-
[Journal Article] Suppression of hepatitis C virus replicon by adenovirus vector-mediated expression of tough decoy RNA against miR-122a. Virus Research2012
Author(s)
Sakurai F, Furukawa N, Higuchi M, Okamoto S, Ono K, Yoshida T, Kondoh M, Yagi K, Sakamoto N, Katayama K, Mizuguchi H
-
Journal Title
EPub ahead of Print
Volume: 165
Issue: 2
Pages: 214-218
DOI
Related Report
Peer Reviewed
-
-
-
-
[Journal Article] Identification of novel N-(morpholine-4-carbonyloxy) amidine compounds as potent inhibitors against hepatitis C virus replication2012
Author(s)
Kusano-Kitazume A, Sakamoto N, Okuno Y, Sekine-Osajima Y, Nakagawa M, Kakinuma S, Kiyonashi K, Nitta S, Murakawa M, Azuma S, Nishimura-Sakurai Y, Hagiwara M, Watanabe M
-
Journal Title
Antimicrob Agent Chemother
Volume: 56(3)
Issue: 3
Pages: 1315-23
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
