| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
CIRP is one of RNA binding proteins, and induced by stress responces, which include moderate cold stress, ultraviolet, and hypoxia. CIRP functions as oncogene, because CIRP induces mouse primary cell immortalization and transformation. To identify CIRP protein-bound mRNA transcripts in whole transcriptome of human hepatoblastoma cell line HepG2, we apply crosslinking and immunoprecipitation(CLIP). CIRP binds mRNA transcripts of Mre11, Rad50, Rad52, NBS, and ATM, that are involved in DNA repair by BRCA1-associated homologous recombination after DNA damage. However, CIRP does not complex with transcripts of Ku80, XRCC4-like, and DNA-PK, that are involved in DNA repair by non-homologous end joining (NHEJ). Spliceosome contains CIRP. CIRP binds phospho-BRCA1 in the nucleus. phospho-BRCA1-CIRP complex undergoes efficient mRNA processing and splicing, and enhances expression of mature transcripts, facilitating resistance against DNA damage.
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