Identification of specific markers for cancer stem cells and anti-cancer therapy targeting cancer stem cells
| Project/Area Number |
24659363
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Kyoto University |
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Principal Investigator |
CHIBA Tsutomu 京都大学, 医学(系)研究科(研究院), 教授 (30188487)
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| Co-Investigator(Kenkyū-buntansha) |
SENO Hiroshi 京都大学, 医学研究科, 講師 (90335266)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 癌幹細胞 / 組織幹細胞 / Hes1 / Dckl1 / 大腸ポリープ / 癌幹細胞治療 / 大腸癌 / 分子標的治療 |
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| Research Abstract |
We examined effects of targeting Hes1 and Dcamkl1, possible cancer stem cell markers, on colonic cancer cells. Hes1-specific siRNAs significantly reduced growth of human colonic cancer cells. In addition, we also examined the effect of Hes1-siRNA to ApcMin mice. Finally, we obtained Dckl1-positive cells from both normal intestine and polyps of ApcMin mice by FACS sorting, and found several transcripts specifically expressed in Dcamkl1-positive tumor cells. The siRNAs or inhibitors specific for the molecules exclusively expressed in Dcamkl1-positive tumor cells inhibited tumor cell growth without affecting normal epithelial cells. These data suggested possible anti-cancer strategy targeting cancer stem cells without affecting normal tissue stem cells.
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Report
(3 results)
Research Products
(40 results)
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[Journal Article] Deletion of nardilysin prevents the development of steatohepatitis and liver fibrosis.2014
Author(s)
Ishizu-Higashi S, Seno H, Nishi E, Matsumoto Y, Ikuta K, Tsuda M, Kimura Y, Takada Y, Kimura Y, Nakanishi Y, Kanda K, Komekado H, Chiba T.
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Journal Title
Plos One
Volume: 9
Issue: 5
Pages: e98017-e98017
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] A model of liver carcinogenesis originating from hepatic progenitor cells with accumulation of genetic alterations.2014
Author(s)
Soo Ki Kim, Akihiro Nasu, Junji Komori, Takahiro Shimizu, Yuko Matsumoto, Yasuko Minaki, Kenji Kohno, Kazuharu Shimizu, Shinji Uemoto, Tsutomu Chiba, Hiroyuki Marusawa.
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Journal Title
International Journal of Cancer
Volume: 134
Issue: 5
Pages: 1067-76
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Gastroesophageal reflux disease symptoms and dietary behaviors are significant correlates of short sleep duration in the general population : The Nagahama Study
Author(s)
Murase K, Tabara Y, Takahashi Y, Muro S, Yamada R, Setoh K, Kawaguchi M, Kadotani H, Kosugi S, Sekine A, Nakayama T, Mishima M, Chiba T, Chin K, Matsuda F.
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URL
Related Report
Peer Reviewed
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