Crosstalk between CagA and inflammation in H. pylori-related gastric carcinogenesis.
Project/Area Number |
24659365
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Single-year Grants |
Research Field |
Gastroenterology
|
Research Institution | Osaka University |
Principal Investigator |
TSUJII Masahiko 大阪大学, 医学(系)研究科(研究院), 准教授 (40303937)
|
Co-Investigator(Kenkyū-buntansha) |
TAKEHARA Tetsuo 大阪大学, 大学院医学系研究科, 教授 (70335355)
IIJIMA Hideki 大阪大学, 大学院医学系研究科, 講師 (90444520)
NISHIDA Tsutomu 大阪大学, 大学院医学系研究科, 助教 (20467575)
|
Project Period (FY) |
2012-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 胃癌 / ヘリコバクターピロリ / CagA / 腸上皮化生 / 胃炎 / let-7 / 炎症 |
Research Abstract |
In in vitro experiments, CagA significantly attenuated let-7 expression leading to Ras pathway activation. CagA enhanced c-myc, DNA methyltransferase 3B (DNMT3B) and Enhancer of Zeste homologue 2 (EZH2) expression and attenuated miR-26a and miR-101 expression, which resulted in the attenuation of let-7 expression by histone and DNA methylation. Experiments performed in CagA transgenic mice revealed that c-myc, EZH2 and DNMT3B expression were enhanced and let-7 expression was attenuated to induce Ras oncoprotein expression in the stomach, with no associated inflammation. It is concluded that H pylori CagA induces aberrant epigenetic silencing of let-7 expression, leading to Ras upregulation.
|
Report
(3 results)
Research Products
(35 results)
-
[Journal Article] Clinical features of post-polypectomy bleeding associated with heparin bridge therapy.2014
Author(s)
Inoue T, Nishida T, Maekawa A, Tsujii Y, Akasaka T, Kato M, Hayashi Y, Yamamoto S, Kondo J, Yamada T, Shinzaki S, Iijima H, Tsujii M, Takehara T.
-
Journal Title
Dig Endosc.
Volume: 26
Issue: 2
Pages: 243-249
DOI
Related Report
Peer Reviewed
-
[Journal Article] Atypical duodenal ulcer and invagination caused by a large pedunculated duodenal Brunner's gland hamartoma.2014
Author(s)
Nakabori T, Shinzaki S, Yamada T, Nishida T, Iijima H, Tsujii M, Kurokawa Y, Mori M, Doki Y, Morii E, Takehara T.
-
Journal Title
Gastrointest Endosc.
Volume: 79
Issue: 4
Pages: 679-680
DOI
Related Report
Peer Reviewed
-
[Journal Article] Concomitant use of romiplostim and chemotherapy for advanced rectal cancer associated with idiopathic thrombocytopenic purpura2014
Author(s)
Yamada M, Shinzaki S, Nishida T, Yamada T, Fujinaga T, Yakushijin T, Tatsumi T, Iijima H, Hiramatsu N, Tsujii M, Takehara T.
-
Journal Title
日本消化器病学会雑誌
Volume: 111
Pages: 521-528
NAID
Related Report
Peer Reviewed
-
[Journal Article] Amelioration of Small Bowel Injury by Switching from Nonselective Nonsteroidal Anti-Inflammatory Drugs to Celecoxib in Rheumatoid Arthritis Patients: A Pilot Study2013
Author(s)
Inoue, T., Iijima, H., Arimitsu, J., Hagihara, K., Kawai, S., Shiraishi, E., Hiyama, S., Mukai, A., Shinzaki, S., Nishida, T., Ogata, A., Tsujii, M., Takehara, T.
-
Journal Title
Digestion
Volume: 89
Issue: 2
Pages: 124-132
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] Acute suppurative pancreatic ductitis associated with pancreatic duct obstruction.2013
Author(s)
Fujinaga T, Nishida T, Miyazaki M, Shigekawa M, Ikezawa K, Iwahashi K, Inoue T, Yamada T, Ezaki H, Shinzaki S, Yakushijin T, Iijima H, Tsujii M, Takehara T.
-
Journal Title
Endoscopy
Volume: 45
Issue: S 02
Pages: 135-135
DOI
Related Report
Peer Reviewed
-
-
-
-
[Journal Article] CagA mediates epigenetic regulation to attenuate let-7 expression in Helicobacter pylori-related carcinogenesis.2013
Author(s)
Hayashi Y, Tsujii M, Wang J, Kondo J, Akasaka T, Jin Y, Li W, Nakamura T, Nishida T, Iijima H, Tsuji S, Kawano S, Hayashi N, Takehara T.
-
Journal Title
GUT
Volume: 62
Issue: 11
Pages: 1536-1546
DOI
Related Report
Peer Reviewed
-
-
-
-
-
[Journal Article] Gastric ESD under Heparin Replacement at High-Risk Patients of Thromboembolism Is Technically Feasible but Has a High Risk of Delayed Bleeding: Osaka University ESD Study Group.2013
Author(s)
Yoshio T, Nishida T, Kawai N, Yuguchi K, Yamada T, Yabuta T, Komori M, Yamaguchi S, Kitamura S, Iijima H, Tsutsui S, Michida T, Mita E, Tsujii M, Takehara T.
-
Journal Title
Gastroenterol Res Pract.
Volume: 2013
Pages: 365830-365830
DOI
Related Report
Peer Reviewed
-
[Journal Article] Narrow Band Imaging with Magnifying Endoscopy for Peyer's Patches in Patients with Inflammatory Bowel Disease2013
Author(s)
Hiyama, S., Iijima, H., Shinzaki, S., Mukai, A., Inoue, T., Shiraishi, E., Kawai, S., Yamada, T., Nishida, T., Tsujii, M., Takehara, T.
-
Journal Title
Digestion
Volume: 87
Issue: 4
Pages: 269-80
DOI
Related Report
Peer Reviewed
-
[Journal Article] Hemobilia into a metallic biliary stent due to pseudoaneurysm: a case report2013
Author(s)
Morishita N, Nishida T, Hayashi Y, Iwahashi K, Ikezawa K, Shigekawa M, Shinzaki S, Yamada T, Kakita N, Ezaki H, Miyazaki M, Yakushijin T, Tatsumi T, Iijima H, Kiso S, Hiramatsu N, Tsujii M, Osuga K, Terashima K, Takehara T.
-
Journal Title
日本消化器病学会雑誌
Volume: 110
Pages: 2127-2135
NAID
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Cyclooxygenase-2の癌幹細胞バイオロジー、DNA異常メチル化に及ぼす影響.ワークショップ;消化管がん分子病態診断学の進歩2012
Author(s)
辻井正彦,近藤純平,赤坂智史,林義人,金瑛,加藤元彦,山本俊祐,井上拓也,山田拓哉,西田勉,渡部健二,飯島英樹,竹原徹郎
Organizer
第8回日本消化管学会
Place of Presentation
仙台
Related Report
-
[Presentation] ESD後の適切なフォローアップ方法~多発胃癌に対するサーベイランスの観点から~ESDフォーラム; Where is our destination?2012
Author(s)
加藤元彦,西田勉,山本克己,北村信次,由雄敏之,中村剛之,小森真人,河相直樹,西原彰浩,中西文彦,中原征則,荻山秀治,木下和郎,辻井正彦,竹原徹郎
Organizer
第8回日本消化管学会
Place of Presentation
仙台
Related Report
-
-
-
-
-
-