Oxidative stress-induced PTC variants in colonic cancer cells

• Project/Area Number: 24659370
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Gastroenterology
• Research Institution: The University of Tokushima
• Principal Investigator: KUWANO Yuki 徳島大学, ヘルスバイオサイエンス研究部, 助教 (00563454)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 選択的スプライシング / 中途ストップコドン / ＳＲタンパク質 / 大腸がん / 遺伝子発現 / PTCバリアント / 機能性RNA

Research Abstract

Aberrant alternative splicing participates in many genetic and acquired diseases including cancer. Oxidative stress specifically facilitated premature stop codon (PTC)-containing isoforms production in colon cancer cell line. Several RNA-binding proteins associated with PTC isoforms and regulated its RNA sutability in the nucleus.

Research Products

• [Journal Article] Oxidative stress-induced truncated serine/arginine-rich splicing factor 3 regulates interleukin-8 production in human colon cancer cells. (2014)
  • Author(s): Kano S, Nishida K, Kurebe H, Nishiyama C, Kita K, Akaike Y, Kajita K, Kurokawa K, Masuda K, Kuwano Y, Tanahashi T, Rokutan K.
  • Journal Title: Am J Physiol Cell Physiol. Volume: 306 (3) Issue: 3 Pages: 250-262
  • DOI: 10.1152/ajpcell.00091.2013
  • Peer Reviewed / Open Access
• [Journal Article] Downregulation of serine/arginine-rich splicing factor 3 induces G1 cell cycle arrest and apoptosis in colon cancer cells. (2014)
  • Author(s): Kurokawa K, Akaike Y, Masuda K, Kuwano Y, Nishida K, Yamagishi N, Kajita K, Tanahashi T, Rokutan K.
  • Journal Title: Oncogene. Volume: 33 Issue: 11 Pages: 1407-17
  • DOI: 10.1038/onc.2013.86
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Truncated serine/arginine-rich splicing factor 3 accelerates cell growth through up-regulating c-Jun expression (2013)
  • Author(s): Kano S, Nisida K, Nishiyama C, Akaike Y, Kajita K, Kurokawa K, Masuda K, Kuwano Y, Tanahashi T, Rokutan K.
  • Journal Title: J Med. Invest Volume: 60 Pages: 228-235
  • NAID: 130004822687
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/24190
  • Peer Reviewed
• [Journal Article] Chronic academic stress increases a group of microRNAs in peripheral blood (2013)
  • Author(s): Honda M, Kuwano Y, Katsuura-Kamano S, Kamezaki Y, Fujita K, Akaike Y, Kano S, Nishida K, Masuda K, Rokutan K
  • Journal Title: PLoS One Volume: 8(10) Issue: 10 Pages: e75960-e75960
  • DOI: 10.1371/journal.pone.0075960
  • Peer Reviewed
• [Journal Article] NF90 in posttranscriptional gene regulation and microRNA biogenesis. (2013)
  • Author(s): Masuda K, Kuwano Y, Nishida K, Rokutan K, Imoto I.
  • Journal Title: Int J Mol Sci. Volume: 14(8) Issue: 8 Pages: 17111-21
  • DOI: 10.3390/ijms140817111
  • Peer Reviewed
• [Journal Article] Application of DNA microarray technology to gerontological studies. (2013)
  • Author(s): Masuda K, Kuwano Y, Nishida K, Rokutan K.
  • Journal Title: Methods Mol Biol. Volume: 1048. Pages: 285-308
  • DOI: 10.1007/978-1-62703-556-9_19
  • ISBN: 9781627035552, 9781627035569
  • Peer Reviewed
• [Journal Article] Chronic inhibition of tumor cell-derived VEGF enhances the malignant phenotype of colorectal cancer cells. (2013)
  • Author(s): Yamagishi N, Teshima-Kondo S, Masuda K, Nishida K, Kuwano Y, Dang DT, Dang LH, Nikawa T, Rokutan K.
  • Journal Title: BMC Cancer Volume: 13 Issue: 1 Pages: 229-229
  • DOI: 10.1186/1471-2407-13-229
  • Peer Reviewed
• [Journal Article] Ets1 and heat shock factor 1 regulate transcription of the Transformer 2β gene in human colon cancer cells. (2013)
  • Author(s): Kajita K, Kuwano Y, Kitamura N, Satake Y, Nishida K, Kurokawa K, Akaike Y, Honda M, Masuda K, Rokutan K.
  • Journal Title: J Gastroenterol. Volume: 48(11) Issue: 11 Pages: 1222-33
  • DOI: 10.1007/s00535-012-0745-2
  • Peer Reviewed
• [Journal Article] Truncated serine/arginine-rich splicing factor 3 accelerates cell growth through up-regulating c-Jun expression. (2013)
  • Author(s): Kano S, Nishida K, Nishiyama C, Akaike Y, Kajita K, Kurokawa K, Masuda K, Kuwano Y, Tanahashi T, Rokutan K.
  • Journal Title: The journal of medical investigation Volume: 60(3-4) Pages: 228-235
  • NAID: 130004822687
  • Peer Reviewed
• [Journal Article] Role of miR-19b and its target mRNAs in 5-fluorouracil resistance in colon cancer cells (2012)
  • Author(s): Kurokawa K, Tanahashi T, Iima T, Yamamoto Y, Akaike Y, Nishida K, Masuda K, Kuwano Y, Murakami Y, Fukushima M, Rokutan
  • Journal Title: J Gastroenterol Volume: 47 Issue: 8 Pages: 883-895
  • DOI: 10.1007/s00535-012-0547-6
  • NAID: 10031051201
  • Peer Reviewed
• [Journal Article] MicroRNAs miR-144/144* and miR-16 in peripheral blood are potential biomarkers for naturalistic stress in healthy Japanese medical students. (2012)
  • Author(s): Katsuura S, Kuwano Y, Yamagishi N, Kurokawa K, Kajita K, Akaike Y, Nishida K, Masuda K, Tanahashi T, Rokutan K.
  • Journal Title: Neurosci Lett. Volume: 516 Issue: 1 Pages: 79-84
  • DOI: 10.1016/j.neulet.2012.03.062
  • Peer Reviewed
• [Presentation] 末梢血の遺伝子発現から見たストレス応答の分子基盤 (2013)
  • Author(s): 桑野由紀
  • Organizer: シンポジウム講演第29回日本ストレス学会学術総会
  • Place of Presentation: 徳島大学・大塚講堂(徳島県)
  • Year and Date: 2013-11-09
• [Presentation] ストレス応答性Transformer 2β遺伝子の発現を介した細胞増殖の調節メカニズム (2013)
  • Author(s): 宮原圭吾、桑野由紀、西田憲生、赤池瑶子、狩野静香、六反一仁
  • Organizer: 第29回日本ストレス学会学術総会
  • Place of Presentation: 徳島大学・大塚講堂(徳島県)
  • Year and Date: 2013-11-08
• [Presentation] 医療従事者を対象とした社会格差による健康障害メカニズムの検討 (2013)
  • Author(s): 藤田絹代、桑野由紀、西田憲生、赤池瑶子、狩野静香、六反一仁
  • Organizer: 第29回日本ストレス学会学術総会
  • Place of Presentation: 徳島大学・大塚講堂(徳島県)
  • Year and Date: 2013-11-08
• [Presentation] Chronic academic stress increases a group of microRNAs in peripheral blood in healthy Japanese students (2013)
  • Author(s): Kuwano Y, Honda M, Fujita K, Akaike Y, Kano S, Satake Y, Nishida K, Rokutan K.
  • Organizer: The international conference on social stratification and health 2013
  • Place of Presentation: 東京大学・鉄門講堂(東京都)
  • Year and Date: 2013-09-01
• [Presentation] Autism-associated gene expression was commonly observed in peripheral blood leukocytes from subjects with autism and helthy mothers having autistic children (2013)
  • Author(s): Kuwano Y, Katsuura S, Kawai T, Kamio Y, Rokutan K.
  • Organizer: 11^<th> World Congress of Biological Psychiatry
  • Place of Presentation: Kyoto International Conference Center (Kyoto, Japan)
  • Year and Date: 2013-06-24
• [Presentation] ヒト末梢血における慢性心理的ストレス応答性マイクロRNAの同定 (2013)
  • Author(s): 桑野由紀、本田真奈美、梶田敬介、赤池瑶子、藤田絹代、佐竹譲、西田憲生、増田清士、六反一仁
  • Organizer: euro 2013
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2013-06-20
• [Presentation] 末梢血の遺伝子発現から見たストレス応答の分子基盤 (2013)
  • Author(s): 桑野由紀
  • Organizer: 第29回日本ストレス学会学術総会
  • Place of Presentation: 大塚講堂（徳島県徳島市）
  • Invited
• [Presentation] ストレス応答性Transformer 2β遺伝子の発現を介した細胞増殖の調節メカニズム (2013)
  • Author(s): 宮原圭吾、桑野由紀、西田憲生、赤池瑶子、狩野静香、六反一仁
  • Organizer: 第29回日本ストレス学会学術総会
  • Place of Presentation: 大塚講堂（徳島県徳島市）
• [Presentation] ヒト末梢血における慢性心理的ストレス応答性マイクロRNAの同定 (2013)
  • Author(s): 桑野由紀、本田真奈美、梶田敬介、赤池瑶子、藤田絹代、佐竹譲、西田憲生、増田清士、六反一仁.
  • Organizer: Neuro2013
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Autism-associated gene expression was commonly observed in peripheral blood leukocytes from subjects with autism and helthy mothers having autistic children. (2013)
  • Author(s): Kuwano Y, Katsuura S, Kawai T, Kamio Y, Rokutan K.
  • Organizer: 11th World Congress of Biological Psychiatry
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Ultraconserved exon-containing SRSF3 mRNA isoform is specifically translated to the truncated SRSF3 protein under oxidative stress (2012)
  • Author(s): Kano S, Nishida K, Kuwano Y, Masuda K, Kurokawa K, Rokutan K.
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2012-12-12
• [Presentation] Transfomer-2beta regulates apoptosis through post-transcriptional regulation of bcl-2 (2012)
  • Author(s): Kuwano Y, Kajita K, Satake Y, Akaike Y, Honda M, Fujita F, Nishida K, Masuda K, Rokutan K.
  • Organizer: Cell symposia-Functional RNAs
  • Place of Presentation: Hotel Melia (Sitges, Spain)
  • Year and Date: 2012-12-03
• [Presentation] 選択的スプライシング因子Tra2βを介したBcl-2の転写後調節 (2012)
  • Author(s): 桑野由紀、梶田敬介、佐竹譲、赤池瑶子、本田真奈美、藤田絹代、西田憲生、増田清士、六反一仁
  • Organizer: 第7回臨床ストレス応答学会大会
  • Place of Presentation: 東京女子医科大学・弥生記念講堂(東京都)
  • Year and Date: 2012-11-24
• [Presentation] 精神的ストレスに応答するヒト末梢血マイクロRNAの発現 (2012)
  • Author(s): 本田真奈美、桑野由紀、佐竹譲、梶田敬介、赤池瑶子、藤田絹代、西田憲生、増田清士、六反一仁
  • Organizer: 第7回臨床ストレス応答学会大会
  • Place of Presentation: 東京女子医科大学・弥生記念講堂(東京都)
  • Year and Date: 2012-11-24
• [Presentation] Ultraconserved element-containing Transformer 2β4 mRNA regulates cellular senescence (2012)
  • Author(s): Kajita K, Kuwano Y, Satake Y, Akaike Y, Honda M, Fujita F, Nishida K, Masuda K, Rokutan K.
  • Organizer: The 4th EMBO Meeting
  • Place of Presentation: Novotel Nice Centre (Nice, France)
  • Year and Date: 2012-09-22
• [Presentation] Transfomer-2beta regulates apoptosis through post-transcriptional regulation of bcl-2 (2012)
  • Author(s): 桑野由紀
  • Organizer: Cell symposia-Functional RNAs-
  • Place of Presentation: Holel Melia (Sitges, Spain)
• [Presentation] 選択的スプライシング因子Tra2βを介したBcl-2の転写後調節 (2012)
  • Author(s): 桑野由紀
  • Organizer: 第７回臨床ストレス応答学会
  • Place of Presentation: 東京女子医科大学 (東京都)