| Project/Area Number |
24659389
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Kyoto University |
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Principal Investigator |
YAMASHITA Jun 京都大学, iPS 細胞研究所, 教授 (50335288)
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| Project Period (FY) |
2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | エピジェネティクス / ES細胞 / 分化(4) G9a / PKA / ヒストンメチル化 / 分化 / G9a |
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| Research Abstract |
Currently, it is still impossible to manipulate the timing and velocity of embryonic stem cell differentiation. This study is aiming at elucidation of molecular and cellular mechanisms of regulation for timing and velocity of stem cell differentiation. We demonstrated that activation of protein kinase A (PKA) accelerates differentiation velocity to induce approximately 2-times earlier appearance of mesoderm and other germ layer cells, reciprocally correlated with the earlier disappearan ce of pluripotent markers after ESC differentiation. PKA activation increased protein expression of G9a, a H3K9 methyltransferase, along with earlier H3K9 dimethylation and DNA methylation in Oct3/4 and Nanog gene promoters. We thus reported this novel mechanism regulating differentiation timing of embryonic stem cells（Yamamizu, Cell Stem Cell, 2012）
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