| Project/Area Number |
24659396
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Niigata University |
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Principal Investigator |
SAIJO YASUO 新潟大学, 医歯学系, 教授 (10270828)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | iPS 細胞 / 肺再生 / 肺障害 / iPS / 肺スキャフォールド / 肺胞上皮細胞 / 分化 |
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| Research Abstract |
We transplanted alveolar differentiated iPS cells into the lung scaffolds. After 12 days culture in vitro, Differentiated iPS cells reconstructed the alveoli and over 10% cells were positive for SPC or Tia. In the bleomycin-induced lung injury model mouse, the cells were transplanted intratracheally. Bronchoalveolar lavage demonstrated IL-6 and TNF-a were drastically suppressed in the differentiated iPS cells group compared to the controls. Fibrotic changes were also suppressed in differentiated iPS cells group. SPC+/PKH26+ or T1a+/PKH26+ cells were observed in the differentiated iPS cells group, but positive cells were quite few. In this study, we demonstrated that alveolar differentiated iPS cells could reconstruct alveoli of the lung scaffold, and ameliorate lung fibrosis in the bleomycin-induced lung injury model mouse.
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