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A challenge of lung regeneration by iPS cells

Research Project

Project/Area Number 24659396
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Respiratory organ internal medicine
Research InstitutionNiigata University

Principal Investigator

SAIJO YASUO  新潟大学, 医歯学系, 教授 (10270828)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsiPS 細胞 / 肺再生 / 肺障害 / iPS / 肺スキャフォールド / 肺胞上皮細胞 / 分化
Research Abstract

We transplanted alveolar differentiated iPS cells into the lung scaffolds. After 12 days culture in vitro, Differentiated iPS cells reconstructed the alveoli and over 10% cells were positive for SPC or Tia. In the bleomycin-induced lung injury model mouse, the cells were transplanted intratracheally. Bronchoalveolar lavage demonstrated IL-6 and TNF-a were drastically suppressed in the differentiated iPS cells group compared to the controls. Fibrotic changes were also suppressed in differentiated iPS cells group. SPC+/PKH26+ or T1a+/PKH26+ cells were observed in the differentiated iPS cells group, but positive cells were quite few. In this study, we demonstrated that alveolar differentiated iPS cells could reconstruct alveoli of the lung scaffold, and ameliorate lung fibrosis in the bleomycin-induced lung injury model mouse.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (10 results)

All 2014 2013 2012

All Journal Article (8 results) (of which Peer Reviewed: 7 results) Presentation (2 results)

  • [Journal Article] Differentiation of Mouse Induced Pluripotent Stem Cells Into Alveolar Epithelial Cells In Vitro for Use In Vivo2014

    • Author(s)
      Zhou Q, Ye X, Sun R, Matsumoto Y, Moriyama M, Asano Y, Ajioka Y, Saijo Y.
    • Journal Title

      Stem Cells Transl Med

      Volume: (Epub ahead of print)

    • Related Report
      2013 Final Research Report
  • [Journal Article] Differentiation of Mouse-induced Pluripotent Stem Cells into Alveolar Epithelial Cells In Vitro for In Vivo2014

    • Author(s)
      Zhou Q, Ye X, Sun R, Matsumoto Y, Moriyama M, Asano Y, Ajioka Y, Saijo Y.
    • Journal Title

      Stem Cells Translational Medicine

      Volume: in press

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Respiratory Society Committee for formulation of Consensus statement for the diagnosis and treatment of drug-induced lung injuries2013

    • Author(s)
      Kubo A, Azuma A, Kanazawa M, Kameda H, et al.
    • Journal Title

      Respir Invest

      Volume: 51 Issue: 4 Pages: 260-277

    • DOI

      10.1016/j.resinv.2013.09.001

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A change in the number of CCSP(pos)/SPC(pos) cells in mouse lung during development, growth, and repair.2013

    • Author(s)
      Sun R, Zhou Q, Ye X, Takahata T, Ishiguro A, Kijima H, Nukiwa T, Saijo Y.
    • Journal Title

      Respir Investig.

      Volume: 51 Issue: 4 Pages: 229-240

    • DOI

      10.1016/j.resinv.2013.04.006

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Randomized phase II trial of uracil/tegafur and cisplatin versusvinorelbine and cisplatin with concurrent thoracic radiotherapy for locally advanced unresectable stage III non-small-cell lung cancer:NJLCG 06012013

    • Author(s)
      Sugawara S, Maemondo M, Tachihara M, Inoue A, Ishimoto O, Sakakibara T, 全14名中6番目
    • Journal Title

      Lung Cancer

      Volume: 81 Issue: 1 Pages: 1-1

    • DOI

      10.1016/j.lungcan.2013.04.010

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Updated Overall Survival Results from A Randomized Phase III Trial Comparing Gefitinib with Carboplatin-Paclitaxel for Chemo-Naive Non-Small Cell Lung Cancer with Sensitive EGFR Gene Mutations (NEJ002)2013

    • Author(s)
      Inoue A, Kobayashi K, Maemondo M, et al
    • Journal Title

      Ann Oncol

      Volume: 24 Issue: 1 Pages: 54-9

    • DOI

      10.1093/annonc/mds214

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Quality of Life with Gefitinib in Patients with EGFR-Mutated Non-Small Cell Lung Cancer : Quality of Life Analysis of North East Japan Study Group 002 Trial2012

    • Author(s)
      Oizumi S, Kobayashi K, Inoue A, Maemondo M, Sugawara S, Yoshizawa H, Isobe H, Harada M, Kinoshita I, Okinaga S, Kato T, Harada T, Gemma A, Saijo Y, Yokomizo Y, Morita S, Hagiwara K, Nukiwa T.
    • Journal Title

      Oncologist

      Volume: 17 Issue: 6 Pages: 863-70

    • DOI

      10.1634/theoncologist.2011-0426

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Methylation of the KEAP1 gene promoter region in human colorectal cancer2012

    • Author(s)
      Hanada N, Takahata T, Zhou Q, Ye X, Sun R, Itoh J, Ishiguro A, Kijima H, Mimura J, Itoh K, Fukuda S, Saijo Y
    • Journal Title

      BMC Cancer

      Volume: 12 Issue: 1 Pages: 66-66

    • DOI

      10.1186/1471-2407-12-66

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] マウスiPS細胞における肺上皮細胞への分化誘導2013

    • Author(s)
      ZHOU QILIANG, YE XULU, SUN RUOWEN,松本吉史、浅野義哉、西條康夫
    • Organizer
      第12回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2013-03-22
    • Related Report
      2013 Final Research Report
  • [Presentation] マウスiPS細胞における肺上皮細胞への分化誘導2013

    • Author(s)
      Qiliang Z, Xulu Y, Ruowen S, Matumoto Y, Saijo Y
    • Organizer
      第12回日本再生医療学会総会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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