Development of in vivo imaging for pulmanary amyloidosis

• Project/Area Number: 24659397
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Respiratory organ internal medicine
• Research Institution: Toho University (2013-2014); Tohoku University (2012)
• Principal Investigator: EBIHARA Satoru 東邦大学, 医学部, 教授 (90323013)
• Co-Investigator(Kenkyū-buntansha): KUDO Yukitsuka 東北大学, 加齢医学研究所, 教授 (80375203)
• Research Collaborator: OKUNI Ikuko ; SATO Ryuhei
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 肺アミロイドーシス / in vivoイメージング / PETプローブ / 近赤外線 / アミロイド / 蛍光プローブ / PET / ブレオマイシン / コンゴレッド染色 / Aβ1-42

Outline of Final Research Achievements

Pulmonary amyloidosis is a disease characterized by extracellular deposition of protein in an abnormal fibrillar form in the lung. The identification of amyloid requires biopsy procedures and Congo red staining that produces green birefringence under polarized light. However, the biopsy procedures have risk such as bleeding. Moreover, Congo red staining of amyloid is not a very sensitive test and requires adequate observer experience. Recently, amyroid imaging for Altzheimer disease has been developed using flurocent probes. These probes might be useful for amyloid imaging for lung amyloidosis. Therefore, the ultimate aim of this study was to estabilsh the method for amyloid imaging for the lung amyloidosis. To achieve this model poupose, we developed a mouse model for pulmonary amyloidosis. Using this model and THK-265, we detected amyloid deposition in the lung. The results suggest that it is feasible to perform amyloid imaging for pulonary amyloidosis patients.

Research Products

• [Journal Article] Chronic inflammation, lymphangiogenesis, and effect of an anti-VEGFR therapy in a mouse model and in human patients with aspiration pneumonia. (2015)
  • Author(s): Nihei M, Okazaki T, Ebihara S, Kobayashi M, Niu K, Gui P, Tamai T, Nukiwa T, Yamaya M, Kikuchi T, Nagatomi R, Ebihara T, Ichinose M.
  • Journal Title: J Pathol Volume: ;235 Issue: 4 Pages: 632-45
  • DOI: 10.1002/path.4473
  • Peer Reviewed / Open Access
• [Journal Article] 加齢による生理変化と老年症候群 (2015)
  • Author(s): 海老原 覚、大国 生幸、鶴岡 広、原田 孝
  • Journal Title: 薬局 Volume: 66 Pages: 37-41
• [Journal Article] Thermal Taste and Anti-Aspiration Drugs: a Novel Drug Discovery against Pneumonia. (2014)
  • Author(s): Ebihara S, Ebihara T, Gui P, Osaka K, Sumi Y, Kohzuki M.
  • Journal Title: Curr Pharm Des. Volume: In press
  • Peer Reviewed
• [Journal Article] Inhibitory effect of cervical trachea and chest wall vibrations on cough reflex sensitivity and perception of urge-to-cough in healthy male never-smokers (2013)
  • Author(s): Kashiwazaki N, Ebihara S, Gui P, Katayama N, Ito K, Sato R, Oyama C, Ebihara T, Kohzuki M.
  • Journal Title: Cough Volume: 9 Issue: 1 Pages: 22-22
  • DOI: 10.1186/1745-9974-9-22
  • Peer Reviewed
• [Journal Article] Royal jelly prevents the progression of sarcopenia in aged mice in vivo and in vitro (2013)
  • Author(s): Kaijun Niu, Hui Guo, Yinting Guo, Satoru Ebihara, Masanori Asada, Takashi Ohrui, Katsutoshi Furukawa, Masakazu Ichinose,Kazuhiko Yanai, Yukitsuka Kudo, Hiroyuki Arai, Tatsuma Okazaki, and Ryoichi Nagatomi
  • Journal Title: Journal of Gerontology: Biological Sciences Volume: (掲載確定) Issue: 12 Pages: 1482-1492
  • DOI: 10.1093/gerona/glt041
  • Peer Reviewed
• [Journal Article] Correlation between pulmonary function and brain volume in healthy elderly subjects (2013)
  • Author(s): Taki Y, Kinomura S, Ebihara S, Thyreau B, Sato K, Goto R, Kakizaki M, Tsuji I, Kawashima R, Fukuda H
  • Journal Title: Neuroradiology Volume: 55 Issue: 6 Pages: 689-695
  • DOI: 10.1007/s00234-013-1157-6
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Adiponectin Pathway Attenuates Malignant Mesothelioma Cell Growth (2012)
  • Author(s): Niu K, Asada M, et al
  • Journal Title: Am J Respir Cell Mol Biol Volume: 46 Issue: 4 Pages: 515-523
  • DOI: 10.1165/rcmb.2011-0068oc
  • Peer Reviewed
• [Presentation] Cough in older people (2014)
  • Author(s): Ebihara S
  • Organizer: European Respiratory Society International Conference
  • Place of Presentation: ミュンヘン（ドイツ）
  • Year and Date: 2014-09-10
  • Invited
• [Presentation] 温度受容体をターゲットとした嚥下改善・抗誤嚥薬開発
  • Author(s): 海老原覚、角保徳、海老原孝枝
  • Organizer: 第87回日本薬理学会年回
  • Place of Presentation: 仙台国際センター(宮城県）
  • Invited
• [Remarks] 東北大学大学委員医学系研究科内部障害学分野
  • URL: http://www.naibu.med.tohoku.ac.jp/