The establishment of new screening method using the secretory protein expression system for the investigation of the causative antigen of the disease mainly composed cell-mediated immune response.
| Project/Area Number |
24659402
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
EISHI Yoshinobu 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (70151959)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 液性免疫反応 / 細胞性免疫反応 / サルコイドーシス / 感染症 / cDNAライブラリ |
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| Research Abstract |
We made Propionibacterium acnes secretory protein expression system and performed the screening using the cells from animals immunized with P. acnes and that system. We performed lymphocyte stimulation test against 1132 clones and got one positive clone. This recombinant protein did not, however, show a positive result with the cells from patients with sarcoidosis. We needed to perform screenings with the cells from patients with disease susceptibility rather than using cells from immunized animals. We then carried out the same system with patients' cells, but we encountered some difficulties due to the limited number. Therefore we executed the screening with patients' blood plasma instead for the first screening to select candidates for antigen. As a result, we had found 5 antigen candidates and have confirmed its induction to cell-mediated immune response with P. acnes immunized mice. Currently, we are validating their reactivity with the sarcoidosis patients.
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Report
(3 results)
Research Products
(45 results)
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[Journal Article] Complete genome sequence of a Propionibacterium acnes isolate from a sarcoidosis patient.2013
Author(s)
Minegishi K, Aikawa C, Furukawa A, Watanabe T, Nakano T, Ogura Y, Ohtsubo Y, Kurokawa K, Hayashi T, Maruyama F, Nakagawa I, Eishi Y
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Journal Title
Genome Announcement
Volume: 1
Issue: 1
Pages: 16-12
DOI
Related Report
Peer Reviewed
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[Journal Article] Localization of propionibacterium acnes in granulomas supports a possible etiologic link between sarcoidosis and the bacterium.2012
Author(s)
Negi M, Takemura T, Guzman J, Uchida K, Furukawa A, Suzuki Y, Iida T, Ishige I, Minami J, Yamada T, Kawachi H, Costabel U, Eishi Y.
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Journal Title
Mod Pathol
Volume: 25(9)
Issue: 9
Pages: 1284-1297
DOI
Related Report
Peer Reviewed
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[Journal Article] Complete genome sequence of the serotype k Strept ococcus mutans strain LJ23.2012
Author(s)
Aikawa C, Furukawa N, Watanabe T, Minegishi K, Furukawa A, Eishi Y, Oshima K, Kurukawa K, Hattori M, Nakano K, Maruyama F, Nakagawa I, Ooshima T
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Journal Title
Journal of Bacteriology
Volume: 194
Issue: 10
Pages: 2754-2755
DOI
Related Report
Peer Reviewed
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[Journal Article] Reduced brain-derived neurotrophic factor (BDNF) mRNA expression and presence of BDNF-immunoreactive granules in the spinocerebellar ataxia type 6 (SCA6) cerebellum2012
Author(s)
Takahashi M, Ishikawa K, Sato N, Obayashi M, Niimi Y, Ishiguro T, Yamada M, Toyoshima Y, Takahashi H, Kato T, Takao M, Murayama M, Mori O, Eishi Y, Mizusawa H
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Journal Title
Neuropathology
Volume: (in press)
Issue: 6
Pages: 593-603
DOI
Related Report
Peer Reviewed
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[Presentation] アクネ菌陽性の組織所見を伴った下垂体Sarcoidosisの一例2013
Author(s)
新山道大,小野昌美,三木伸泰,鈴木薫,渡辺大輔,森本聡,磯崎収,天野耕作,小森隆司,岡田芳和,江石義信,市原淳弘
Organizer
第86回日本内分泌学会学術総会
Place of Presentation
仙台
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