| Project/Area Number |
24659414
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Kidney internal medicine
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| Research Institution | Niigata University |
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Principal Investigator |
FUJINAKA HIDEHIKO 新潟大学, 医歯(薬)学総合研究科, 非常勤講師 (20447642)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Tadashi 新潟大学, 医歯学系, 教授 (30092737)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 遠位尿細管 / 近位尿細管 / Calbindin 1 / the human protein atlas / microarray / Western blotting / データベース / 尿プロテオーム / 免疫染色 / ウエスタンブロッティング / 間質尿細管傷害 / IgA腎症 / UUO / バイオマーカー / 尿蛋白質 / 尿細管 |
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| Outline of Final Research Achievements |
The aim of the study is to discover urinary biomarkers which identify the kidney injured sites as distal or proximal tubules, glomeruli, and interstitium. The proteome-transcriptome-combined database was completed with 3 databases; the Human Protein Atlas (immunohisotochemistry), the microarray database of human kidney, and the urinary proteome database. AS a result, 14 proteins were defined as distal originated urinary proteins, and 50 proteins were proximal. In rat UUO kidneys, mRNA expressions of 8 of 14 distal proteins such as ANXA3 were up-regulated while 3 of 14 distal proteins such as CALB1 were down-regulated. Urinary CALB1 were shown reduced in anti-GBM GN rats with significant distal tubule cells injury, and in some IgA nephropathy patients with tubulointerstitial injury. Our proteome-transcriptome-combined database may be a good tool to discover new urinary biomarkers which identify the injured sites as distal or proximal tubules.
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