Detection of injured nephron segments using brand-new urinary biomarkers
| Project/Area Number |
24659414
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Kidney internal medicine
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| Research Institution | Niigata University |
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Principal Investigator |
FUJINAKA HIDEHIKO 新潟大学, 医歯(薬)学総合研究科, 非常勤講師 (20447642)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Tadashi 新潟大学, 医歯学系, 教授 (30092737)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 遠位尿細管 / 近位尿細管 / Calbindin 1 / the human protein atlas / microarray / Western blotting / データベース / 尿プロテオーム / 免疫染色 / ウエスタンブロッティング / 間質尿細管傷害 / IgA腎症 / UUO / バイオマーカー / 尿蛋白質 / 尿細管 |
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| Outline of Final Research Achievements |
The aim of the study is to discover urinary biomarkers which identify the kidney injured sites as distal or proximal tubules, glomeruli, and interstitium. The proteome-transcriptome-combined database was completed with 3 databases; the Human Protein Atlas (immunohisotochemistry), the microarray database of human kidney, and the urinary proteome database. AS a result, 14 proteins were defined as distal originated urinary proteins, and 50 proteins were proximal. In rat UUO kidneys, mRNA expressions of 8 of 14 distal proteins such as ANXA3 were up-regulated while 3 of 14 distal proteins such as CALB1 were down-regulated. Urinary CALB1 were shown reduced in anti-GBM GN rats with significant distal tubule cells injury, and in some IgA nephropathy patients with tubulointerstitial injury. Our proteome-transcriptome-combined database may be a good tool to discover new urinary biomarkers which identify the injured sites as distal or proximal tubules.
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] Decreased urinary calbindin 1 levels in proteinuric rats and humans with distal nephron segment injuries.2014
Author(s)
Iida T, Fujinaka H, Xu B, Zhang Y, Magdeldin S, Nameta M, Liu Z, Yoshida Y, Yaoita E, Tomizawa S, Saito A, Yamamoto T
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Journal Title
Clin Exp Nephrol.
Volume: 18
Issue: 3
Pages: 432-43
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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