Establishment of a system for monitoring endoplasmic reticulum redox state in mammalian cells
Project/Area Number |
24659429
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Single-year Grants |
Research Field |
Neurology
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Research Institution | Nagoya University |
Principal Investigator |
ISHIGAKI Shinsuke 名古屋大学, 医学(系)研究科(研究院), 助教 (40378170)
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Project Period (FY) |
2012-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | ER stress / 神経変性疾患 / ALS / 小胞体ストレス |
Research Abstract |
The endoplasmic reticulum (ER) performs a critical role in the oxidative folding of nascent proteins such that perturbations to ER homeostasis may lead to protein misfolding and subsequent pathological processes. Among the mechanisms for maintaining ER homeostasis is a redox regulation, which is a critical determinant of the fate of ER stressed cells. Here we report the establishment of a system for monitoring ER redox state in mammalian cells. The new ER redox sensing system was developed based on the previously described monitoring system in yeast. Our system could successfully monitor the dynamic ER redox state in mammalian cells. Using this system, we find that manipulation of ER oxidases changes ER redox state. The mammalian ER redox sensing system could be used to study the mechanisms of ER redox regulation and provide a foundation for an approach to develop novel therapeutic modalities for human diseases related to dysregulated ER homeostasis including neurodegeneration.
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Report
(3 results)
Research Products
(23 results)
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[Journal Article] Lower Motor Neuron Involvement in TAR DNA-Binding Protein of 43 kDa-Related Frontotemporal Lobar Degeneration and Amyotrophic Lateral Sclerosis2014
Author(s)
Riku Y, Watanabe H, Yoshida M, Tatsumi S, Mimuro M, Iwasaki Y, Katsuno M, Iguchi Y, Masuda M, Senda J, Ishigaki S, Udagawa T, Sobue G.
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Journal Title
JAMA Neurol
Volume: 71(2)
Pages: 172-9
DOI
Related Report
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[Journal Article] Lower Motor Neuron Involvement in TAR DNA-Binding Protein of 43 kDa-Related Frontotemporal Lobar Degeneration and Amyotrophic Lateral Sclerosis.2014
Author(s)
Riku Y, Watanabe H, Yoshida M, Tatsumi S, Mimuro M, Iwasaki Y, Katsuno M, Iguchi Y, Masuda M, Senda J, Ishigaki S, Udagawa T, Sobue G.
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Journal Title
JAMA Neurol.
Volume: 71
Pages: 172-9
DOI
Related Report
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[Journal Article] Thioredoxin-interacting protein mediates ER stress-inducedβcell death through initiation of the inflammasome2012
Author(s)
Oslowski CM, Hara T, O'Sullivan-Murphy B, Kanekura K, Lu S, Hara M, Ishigaki S, Zhu LJ, Hayashi E, Hui ST, Greiner D, Kaufman RJ, Bortell R, Urano F.
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Journal Title
Cell Metab
Volume: 16(2)
Pages: 265-73
DOI
Related Report
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[Journal Article] Thioredoxin-interacting protein mediates ER stress-induced β cell death through initiation of the inflammasome.2012
Author(s)
Oslowski CM, Hara T, O'Sullivan-Murphy B, Kanekura K, Lu S, Hara M, Ishigaki S, Zhu LJ, Hayashi E, Hui ST, Greiner D, Kaufman RJ, Bortell R, Urano F.
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Journal Title
Cell Metab.
Volume: 16
Pages: 265-73
DOI
Related Report
Peer Reviewed
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