Induction of regulatory microglia and its therapeutic development

• Project/Area Number: 24659430
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Neurology
• Research Institution: Nagoya University
• Principal Investigator: MIZUNO Tetsuya 名古屋大学, 環境医学研究所, 准教授 (70335008)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: ミクログリア / IL-34 / TGF / 神経保護作用

Research Abstract

Microglia have a biphasic neurotoxic or neuroprotective role in the pathogenesis of various neurodegenerative diseases. In this study, we examined the method for microglial differentiation into neuroprotective cells. We clarified that treatment of IL-34 and TGF for 7 days induces neuroprotective property in microglia, which exert neuroprotection against amyloid beta toxicity.

Research Products

• [Journal Article] Oligomeric amyloid β induces IL-1β processing via production of ROS: implication in Alzheimer's disease. (2013)
  • Author(s): B Parajuli, Y Sonobe, H Horiuchi, H Takeuchi, T Mizuno, A Suzumura
  • Journal Title: Cell Death & Disease Volume: 4 Issue: 12 Pages: e975-e975
  • DOI: 10.1038/cddis.2013.503
  • Peer Reviewed
• [Journal Article] GM-CSF increases LPS-induced production of proinflammatory mediators via upregulation of TLR4 and CD14 in murine microglia. (2013)
  • Author(s): Bijay Parajuli, Yoshifumi Sonobe, Jun Kawanokuchi, Yukiko Doi, Mariko Noda, Hideyuki Takeuchi, Tetsuya Mizuno, Akio Suzumura
  • Journal Title: Journal of Neuroinflammation Volume: 9(1) Issue: 1 Pages: 268-268
  • DOI: 10.1186/1742-2094-9-268
  • Peer Reviewed
• [Journal Article] TGF-β induced by interleukin-34-stimulated microglia regulates microglial proliferation and attenuates oligomeric amyloid β neurotoxicity (2012)
  • Author(s): Ma D, Doi Y, Jin S, Li E, Sonobe Y, Takeuchi H, Mizuno T, Suzumura A
  • Journal Title: Neuroscience Letters Volume: 529(1) Issue: 1 Pages: 86-91
  • DOI: 10.1016/j.neulet.2012.08.071
  • Peer Reviewed
• [Journal Article] The neuroprotective effects of milk fat globule-EGF factor 8 against oligomeric amyloidβtoxicity (2012)
  • Author(s): Li E, Noda M, Doi Y, Parajuli B, Kawanokuchi J, Sonobe Y, Takeuchi H, Mizuno T, Suzumura A.
  • Journal Title: Journal of neuroinflamation Volume: 9(1186/1742-2094-9-148) Pages: 148-148
• [Journal Article] The neuroprotective effects of milk fat globule-EGF factor 8 against oligomeric amyloid β toxicity. (2012)
  • Author(s): Li E, Noda M, Doi Y, Parajuli B, Kawanokuchi J, Sonobe Y, Takeuchi H, Mizuno T, Suzumura A.
  • Journal Title: Journal of neuroinflamation Volume: 9 Pages: 148-148
  • Peer Reviewed
• [Presentation] アルツハイマー病の慢性炎症機序におけるミクログリアの役割 (2013)
  • Author(s): 水野哲也
  • Organizer: 第32回日本認知症学会
  • Place of Presentation: 松本
• [Presentation] 可溶性Aβオリゴマーによるミクログリアの神経傷害作用 (2013)
  • Author(s): PARAJULI Bijay、水野哲也、竹内英之、錫村明生
  • Organizer: 第25回日本神経免疫学会学術集会
  • Place of Presentation: (下関)(口演)
• [Presentation] 可溶性Aβオリゴマーによるミクログリアの神経傷害作用. (2013)
  • Author(s): Parajuli B, 水野哲也
  • Organizer: 第25回日本神経免疫学会学術集会
  • Place of Presentation: 下関
• [Presentation] The neuroprotective role of microglia treated with milk fat globule-EGF factor 8 (2012)
  • Author(s): MIZUNO Tetsuya, NODA Mariko, LI Endong, DOI Yukiko, PARAJULI Bijay, KAWANOKUCHI Jun, SONOBE Yoshifumi, TAKEUCHI Hideyuki, SUZUMURA Akio
  • Organizer: 11th International Congress of Neuroimmunology
  • Place of Presentation: (Boston, USA) (ポスター)
• [Presentation] Milk fat globule-EGF factor 8のAbeta神経毒性に対する作用の検討 (2012)
  • Author(s): 水野哲也、野田万理子、土井由紀子、錫村明生
  • Organizer: 第53回神経学会学術集会
  • Place of Presentation: (東京)(ポスター)
• [Presentation] The neuroprotective role of microglia treated with MFG-E8 (2012)
  • Author(s): Tetsuya Mizuno
  • Organizer: 11th International Congress of Neuroimmunology
  • Place of Presentation: Boston, USA