| Project/Area Number |
24659434
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurology
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| Research Institution | Osaka City University |
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Principal Investigator |
TOMIYAMA Takami 大阪市立大学, 医学(系)研究科(研究院), 准教授 (10305633)
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| Co-Investigator(Kenkyū-buntansha) |
UMEDA Tomohiro 大阪市立大学, 医学研究科, 助教 (70549790)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | マイクロダイアリシス / アルツハイマー病 / トランスジェニックマウス / Aβ / タウ / アミロイドβ / 病理伝播 |
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| Research Abstract |
The purpose of this study is to examine the possible roles of soluble Abeta and tau released from neurons into the extracellular space in the transmission of Alzheimer pathology using the brain microdialysis technique. In a preliminary experiment, only very low levels of tau were detected by microdialysis in the hippocampus of our tau transgenic (Tg) mice. To generate a better donor of soluble Abeta and tau, we crossbred our APP Osaka mutant Tg mice and wild-type tau Tg mice. The resultant double Tg mice showed Abeta oligomer accumulation, tau hyperphosphorylation, synapse loss, and memory impairment at 6 months, and neurofibrillary tangle formation and neuronal loss at 18 months in the absence of tau mutations, all of which appeared earlier than in the parent Tg mice. This mouse model would be a useful tool for investigation of the intercellular propagation of amyloid and tau pathologies.
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