Systematic survey for biologically active peptides in pheochromocytoma tissue.

• Project/Area Number: 24659452
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Endocrinology
• Research Institution: University of Miyazaki
• Principal Investigator: KAZUO Kitamura 宮崎大学, 医学部, 教授 (50204912)
• Co-Investigator(Kenkyū-buntansha): KUWASAKO Kenji 宮崎大学, フロンティア科学実験総合センター, 准教授 (20381098) ; NAGATA Sayaka 宮崎大学, 医学部, 特任助教 (00452920)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 褐色細胞腫 / 受容体 / 新規生理活性ペプチド / cAMP / アミド化 / 血管作動性物質 / Big angiotensin-25 / ラジオイムノアッセイ / Big angiotensin-25 / アンジオテンシン

Outline of Final Research Achievements

We have performed the systematic survey for biologically active peptides in human pheochromocytoma tissue, using assay systems which we had originally established. We have purified and determined more than 50 peptides from human pheochromocytoma tissue. We discovered Big angiotensin-25 from human urine as an endogenous peptide. Further we have developed the assay system for Big angiotensin-25.

Research Products

• [Journal Article] Biological properties of adrenomedullin conjugated with polyethylene glycol. (2014)
  • Author(s): Kubo K, Tokashiki M, Kuwasako K, Tamura M, Tsuda S, Kubo S, Yoshizawa KK, Kato J, Kitamura K.
  • Journal Title: Peptides Volume: 57 Pages: 118-121
  • DOI: 10.1016/j.peptides.2014.05.005
  • Peer Reviewed / Open Access
• [Journal Article] Adrenomedullin does not contribute toward the development of abdominal aortic aneurysm in mice. (2014)
  • Author(s): Sakamoto S, Tsuruda T, Hatakeyama K, Shimosawa T, Asada Y, Kitamura K.
  • Journal Title: Health Volume: 6 Issue: 10 Pages: 1077-1084
  • DOI: 10.4236/health.2014.610133
  • Peer Reviewed / Open Access
• [Journal Article] レニン・アンジオテンシン・アルドステロン系研究の新展開、プロアンジオテンシン-12研究の最前線． (2014)
  • Author(s): 加藤丈司、桑迫健二、小松洋一、永田さやか、北村和雄
  • Journal Title: Angiology Frontier Volume: 13 Pages: 13-18
  • Peer Reviewed
• [Journal Article] レニン・アンジオテンシン系の新展開：ヒト尿中の主要なアンジオテンシン関連ペプチド「ビッグアンジオテンシン-25」の発見． (2014)
  • Author(s): 永田さやか、北村和雄
  • Journal Title: 血管 Volume: 37 Pages: 73-79
  • Peer Reviewed
• [Journal Article] レニン・アンジオテンシン・アルドステロン (RAA) 系 2. ビッグアンジオテンシン-25: ヒト尿中の主要なアンジオテンシン関連ペプチド． (2014)
  • Author(s): 永田さやか、北村和雄
  • Journal Title: 最新医学 Volume: 69 Pages: 2276-2286
  • Peer Reviewed
• [Journal Article] Functions of third extracellular loop and helix 8 of Family B GPCRs complexed with RAMPs and characteristics of their receptor trafficking. (2013)
  • Author(s): Kuwasako, K., et al.,
  • Journal Title: Curr Protein Pept Sci, Volume: 14(5) Pages: 416-28
  • Peer Reviewed
• [Journal Article] Big angiotensin-25: a novel glycosylated angiotensin-related peptide isolated from human urine. (2013)
  • Author(s): Nagata, S., et al.,
  • Journal Title: Biochem Biophys Res Commun, Volume: 441(4) Pages: 757-62
  • Peer Reviewed
• [Journal Article] Effects of proangiotensin-12 infused continuously over 14 days in conscious rats. (2012)
  • Author(s): Komatsu Y, Kida N, Nozaki N, Kuwasako K, Nagata S, Kitamura K, and Kato J.
  • Journal Title: Eur J Pharmacol Volume: 683 Pages: 186-189
  • Peer Reviewed
• [Journal Article] Inhibition of development of abdominal aortic aneurysm by glycolysis restriction. (2012)
  • Author(s): Tsuruda T, Hatakeyama K, Nagamachi S, Sekita Y, Sakamoto S, Endo GJ, Nishimura M, Matsuyama M, Yoshimura K, Sato Y, Onitsuka T, Imamura T, Asada Y, and Kitamura K.
  • Journal Title: Arterioscler Thromb Vasc Biol Volume: 32 Pages: 1410-1417
  • Peer Reviewed
• [Presentation] アンジオテンシン関連ペプチドの新展開． (2014)
  • Author(s): 北村和雄、永田さやか
  • Organizer: 第37回日本高血圧学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-10-17 – 2014-10-19
• [Presentation] アンジオテンシノーゲンの14番目アミノ酸に結合するN型糖鎖の構造解析． (2014)
  • Author(s): 永田さやか、北村和雄
  • Organizer: 第37回日本高血圧学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-10-17 – 2014-10-19
• [Presentation] Twenty years from adrenomedullin discovery: From basic study to translational research. (2014)
  • Author(s): Kitamura K
  • Organizer: The Conference on Bioactive Peptides for Cell-Cell Communication 2014
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2014-09-10 – 2014-09-12
  • Invited
• [Presentation] Novel functions of two G protein-coupled receptor kinases: their marked inhibitory effect on adrenomedullin type 1 receptor signaling. (2014)
  • Author(s): Kuwasako K, Kitamura K, Nagata S, Kato J
  • Organizer: The Conference on Bioactive Peptides for Cell-Cell Communication 2014
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2014-09-10 – 2014-09-12
• [Presentation] Big angiotensin-25: a novel glycosylated angiotensin-related peptide isolated from human urine. (2014)
  • Author(s): Nagata S, Kitamura K
  • Organizer: The Conference on Bioactive Peptides for Cell-Cell Communication 2014
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2014-09-10 – 2014-09-12
• [Presentation] Biological properties of adrenomedullin conjugated with polyethylene glycol. (2014)
  • Author(s): Kubo K, Tokashiki M, Kuwasako K, Tamura M, Tsuda S, Kubo S, Yoshizawa KK, Kato J, Kitamura K.
  • Organizer: he European Society of Cardiology Congress 2014
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2014-08-30 – 2014-09-03
• [Presentation] Big angiotensin-25: a novel glycosylated angiotensin-related peptide isolated from human urine． (2013)
  • Author(s): Nagata S, Hatakeyama K, Asami M, Hibino H, Nishiuchi Y, Kuwasako K, Kato J, Asada Y, Kitamura K
  • Organizer: 第36回日本高血圧学会総会
  • Place of Presentation: 大阪
• [Presentation] ヒト尿中の主要なアンジオテンシン関連ペプチドBig angiotensin-25の単離・同定． (2013)
  • Author(s): 永田さやか、北村和雄
  • Organizer: 第36回日本高血圧学会総会
  • Place of Presentation: 大阪
• [Presentation] アドレノメデュリンの診断薬・治療薬としての可能性 (2012)
  • Author(s): 北村和雄
  • Organizer: 第16回日本心血管内分泌代謝学会学術総会
  • Place of Presentation: 東京
• [Patent(Industrial Property Rights)] 急性腎障害に起因する多臓器不全の予防又は治療薬 (2014)
  • Inventor(s): 北村和雄，池田正浩、園田紘子，加藤丈司
  • Industrial Property Rights Holder: 国立大学法人宮崎大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-036815
  • Filing Date: 2014-02-27
• [Patent(Industrial Property Rights)] 長時間作用型アドレノメデュリン誘導体 (2014)
  • Inventor(s): 北村和雄，加藤丈司，久保恵是，桑迫健二，久保茂，熊谷久美子
  • Industrial Property Rights Holder: 国立大学法人宮崎大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2014-058225
  • Filing Date: 2014-03-20
• [Patent(Industrial Property Rights)] 動脈硬化の検査方法 (2013)
  • Inventor(s): 北村和雄，浅田祐士郎，山下篤，松浦祐之介
  • Industrial Property Rights Holder: 国立大学法人宮崎大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-04-02
• [Patent(Industrial Property Rights)] 新規ペプチドおよびその用途 (2013)
  • Inventor(s): 北村和雄、浅田祐士郎，永田さやか
  • Industrial Property Rights Holder: 国立大学法人宮崎大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-11-06
• [Patent(Industrial Property Rights)] 新規ペプチドおよびその用途 (2013)
  • Inventor(s): 北村和雄
  • Industrial Property Rights Holder: 宮崎大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-033651
  • Filing Date: 2013-02-28