| Project/Area Number |
24659454
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Endocrinology
|
|---|
| Research Institution | Keio University |
|---|
Principal Investigator |
ITOH Hiroshi 慶應義塾大学, 医学部, 教授 (40252457)
|
|---|
| Project Period (FY) |
2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
|
|---|
| Keywords | ES細胞 / 細胞分化 / 腎臓細胞 / メカニカルストレス |
|---|
| Research Abstract |
Our objective was to establish a method for inducing the differentiation of renal tubular cells from human ES cells, and to clarify the significance of mechanical stress in the process of differentiation. KSP was used as a marker for renal tubular cell differentiation. Differentiation to an intermediate mesoderm was promoted using GSK3β inhibitor. Epithelization was then promoted using a reduced serum medium containing EGF, which successfully yielded approximately 5% KSP-positive cells. We then investigated the influence of mechanical stress on cell differentiation using HKC-8, a human proximal tubular cell, under different conditions. However, it was not possible to apply sufficient mechanical stress. We are therefore planning to conduct further investigations in the future.
|
