Pathophysiological elucidation of refractory leukemias with the genetic analyses at the single-cell level

• Project/Area Number: 24659457
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Hematology
• Research Institution: The University of Tokyo
• Principal Investigator: KUROKAWA Mineo 東京大学, 医学部附属病院, 教授 (80312320)
• Project Period (FY): 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
• Keywords: 白血病幹細胞 / BCR-ABL / clonal evolution / AML1-MDS1-Evi1

Research Abstract

In this study, we assessed the variations of BCR-ABL expression at the single-cell level, with the use of patient samples from a t(3;21) positive chronic myeloid leukemia patient and a single-cell gene expression analyzer. We assessed several cell populations of patient samples, isolated via surface antigen profiles. In the result, we found that the expression level of BCR-ABL in CD34+/ CD38-/CD90+ leukemic cells was likely to be lower than those in other fractions at the single-cell level.

Research Products

• [Journal Article] The shortest isoform of C/EBPβ, Liver inhibitory protein (LIP) , collaborates with Evi1 to induce AML in a mouse BMT model (2013)
  • Author(s): Okochi, N., Yoshimi, A., Sato, T., Ikeda, T., Kumano, K., Taoka, K., Sato, Y., Shinohara, A., Tsuruta, A., Masuda, A., Yokota, H., Yatomi, Y., Takahashi, K., Kitaura, J., Kitamura, T. and Kurokawa, M
  • Journal Title: Blood Volume: 121 Issue: 20 Pages: 4142-4155
  • DOI: 10.1182/blood-2011-07-368654
  • NAID: 10031196292
  • Peer Reviewed
• [Journal Article] The role of HLA-matched unrelated transplantation in adult patients with Ph chromosome-negative ALL in first remission. A decision analysis. (2013)
  • Author(s): Kako S
  • Journal Title: Bone Marrow Transplantation Volume: 未定 Issue: 8 Pages: 1077-1083
  • DOI: 10.1038/bmt.2013.4
  • Peer Reviewed
• [Journal Article] Impact of a donor source on adult Philadelphia chromosome-negative acute lymphoblastic leukemia: a retrospective analysis from the Adult Acute Lymphoblastic Leukemia Working Group of the Japan Society for Hematopoietic Cell Transplantation. (2013)
  • Author(s): Nishiwaki S
  • Journal Title: Annals of Oncology Volume: 未定 Issue: 6 Pages: 1594-1602
  • DOI: 10.1093/annonc/mds655
  • Peer Reviewed
• [Journal Article] Clinical evaluation of WT1 mRNA expression levels in peripheral blood and bone marrow in patients with myelodysplastic syndromes. (2013)
  • Author(s): Ueda Y
  • Journal Title: Leukemia & Lymphoma Volume: 未定 Issue: 7 Pages: 1450-1458
  • DOI: 10.3109/10428194.2012.745074
  • Peer Reviewed
• [Journal Article] Erythroleukemia showing early erythroid and cytogenetic responses to azacitidine therapy (2013)
  • Author(s): Sho Hangai
  • Journal Title: Annals of Hematology Volume: 92 Issue: 5 Pages: 707-709
  • DOI: 10.1007/s00277-012-1603-2
  • Peer Reviewed
• [Journal Article] Early-onset Therapy-related Myelodysplastic Syndrome Originating from Prolonged Myelosuppression after Fludarabine-based Therapy (2012)
  • Author(s): Yamazaki S
  • Journal Title: Internal Medicine Volume: 51 Issue: 24 Pages: 3427-3430
  • DOI: 10.2169/internalmedicine.51.8310
  • NAID: 130003308168
  • ISSN: 0918-2918, 1349-7235
  • Peer Reviewed
• [Journal Article] Generation of induced pluripotent stem cells from primary chronic myelogenous leukemia (2012)
  • Author(s): Keiki Kumano
  • Journal Title: BLOOD Volume: 119 Issue: 26 Pages: 6234-6242
  • DOI: 10.1182/blood-2011-07-367441
  • Peer Reviewed
• [Journal Article] Evaluation of procalcitonin with liquid-phase binding assay in hematological malignancy. (2012)
  • Author(s): Koya J
  • Journal Title: Clinica Chimica Acta Volume: 413 Issue: 19-20 Pages: 1633-1636
  • DOI: 10.1016/j.cca.2012.05.003
  • Peer Reviewed
• [Journal Article] Prdm3 and Prdm16 are H3K9me1 methyltransferases required for mammalian heterochromatin integrity. (2012)
  • Author(s): Pinheiro I
  • Journal Title: Cell Volume: 150 Issue: 5 Pages: 948-960
  • DOI: 10.1016/j.cell.2012.06.048
  • Peer Reviewed
• [Journal Article] Thalidomide maintenance therapy for patients with multiple myeloma: meta-analysis. (2012)
  • Author(s): Kagoya Y
  • Journal Title: Leukemia Research Volume: 36 Issue: 8 Pages: 1016-1021
  • DOI: 10.1016/j.leukres.2012.04.001
  • Peer Reviewed
• [Journal Article] Intensive chemotherapy for elderly patients with acute myelogeneous leukemia: a propensity score analysis by the Japan Hematology and Oncology Clinical Study Group (J-HOCS). (2012)
  • Author(s): Oshima K
  • Journal Title: Annals of Hematology Volume: 91 Issue: 10 Pages: 1533-1539
  • DOI: 10.1007/s00277-012-1487-1
  • Peer Reviewed
• [Journal Article] Multiple phosphorylation sites are important for RUNX1 activity in early hematopoiesis and T-cell differentiation. (2012)
  • Author(s): Yoshimi M, Goyama S, Kawazu M, Nakagawa M, Ichikawa M, Imai Y, Kumano K, Asai T, Mulloy JC, Kraft AS, Takahashi T, Shirafuji N, Kurokawa M.
  • Journal Title: Eur J Immunol. Volume: 42 Issue: 4 Pages: 1044-1050
  • DOI: 10.1002/eji.201040746
  • Peer Reviewed
• [Journal Article] Primary MALT lymphoma of the urinary bladder in the background of interstitial cystitis (2012)
  • Author(s): Morita K, Nakamura F, Nannya Y, Nomiya A, Arai S, Ichikawa M, Homma Y, Kurokawa M.
  • Journal Title: Annals of Hematology in press Volume: 91 Issue: 9 Pages: 1505-1506
  • DOI: 10.1007/s00277-012-1419-0
  • Peer Reviewed
• [Journal Article] Treatment of primary intraocular lymphoma with rituximab, high dose methotrexate, procarbazine, and vincristine chemotherapy, reduced whole-brain radiotherapy, and local ocular therapy (2012)
  • Author(s): Taoka K
  • Journal Title: Br J Haematol Volume: 157 Issue: 2 Pages: 252-254
  • DOI: 10.1111/j.1365-2141.2011.08938.x
  • Peer Reviewed
• [Journal Article] Ecotropic viral integration site 1, stem cell self-renewal and leukemogenesis. (2012)
  • Author(s): Kataoka K
  • Journal Title: Cancer Science Volume: 103 Issue: 8 Pages: 1371-1377
  • DOI: 10.1111/j.1349-7006.2012.02303.x
  • Peer Reviewed
• [Journal Article] Lymphoma with specific affinity to endocrine organs (2011)
  • Author(s): 森田剣
  • Journal Title: Ann Hematol. Volume: Nov 22 Issue: 7 Pages: 1149-1150
  • DOI: 10.1007/s00277-011-1372-3
  • Peer Reviewed
• [Presentation] iPS細胞を用いた造血器腫瘍研究 (2013)
  • Author(s): 黒川峰夫
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2013-03-21
• [Presentation] iPS細胞を用いた造血器腫瘍研究 (2013)
  • Author(s): 黒川 峰夫
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: パシフィコ横浜
  • Invited
• [Presentation] 血液疾患とiPS細胞 (2012)
  • Author(s): 黒川峰夫
  • Organizer: 第74回日本血液学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2012-10-20
• [Presentation] Generation of induced pluripotent stem cells from primary hematological malignancies (2012)
  • Author(s): 黒川峰夫
  • Organizer: International Society for Stem Cell Research (ISSCR) 10th annual meeting.
  • Place of Presentation: Yokoyama
• [Presentation] Regulation of Chronic myeloid leukemia stem cells by leukemia oncogene Evi1 (2012)
  • Author(s): 黒川峰夫
  • Organizer: 17th congress of EHA.
  • Place of Presentation: Amsterdam
• [Presentation] NF-κB/TNF-α Positive Feedback Loop with Active Proteasome Machinery Supports Myeloid Leukemia Initiating Cell Capacity (2012)
  • Author(s): 黒川峰夫
  • Organizer: 54th ASH Annual Meeting
  • Place of Presentation: Atlanta
• [Presentation] Inhibition of EZH2 Depletes MLL Fusion Leukemia Stem Cells Through Restoration of p16 Expression (2012)
  • Author(s): 黒川峰夫
  • Organizer: 54th ASH Annual Meeting.
  • Place of Presentation: Atlanta
• [Presentation] CD41 and Mpl Positive Fraction Shows an Efficient Leukemia-Initiating Capacity in Evi1-Induced Leukemia (2012)
  • Author(s): 西川慧、荒井俊也、大河内直子、黒川峰夫
  • Organizer: 54th ASH Annual Meeting.
  • Place of Presentation: Atlanta
• [Presentation] The Serial Profile of Vitamins and Mineral Microelements During the Acute Phase of Hematopoietic Stem Cell Transplantation (2012)
  • Author(s): 南谷泰仁、篠原明仁、市川幹、黒川峰夫
  • Organizer: 54th ASH Annual Meeting
  • Place of Presentation: Atlanta
• [Presentation] 血液疾患とiPS細胞 (2012)
  • Author(s): 黒川 峰夫
  • Organizer: 第74回日本血液学会学術集会
  • Place of Presentation: 国立京都国際会館
  • Invited
• [Remarks]
  • URL: http://www.u-tokyo-hemat.com/