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Development of a novel anti-platelet drug targeted for collagen-induced platelet aggregation

Research Project

Project/Area Number 24659460
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Hematology
Research InstitutionKanazawa University

Principal Investigator

YOSHIDA Shigeto  金沢大学, 薬学系, 教授 (10296121)

Co-Investigator(Kenkyū-buntansha) IYORI Mitsuhito  金沢大学, 薬学系, 助教 (20608351)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords抗血小板薬 / コラーゲン / ハマダラカ / 唾液タンパク / 結晶構造
Research Abstract

AAPP, a mosquito saliva protein, specifically blocks platelet adhesion to collagen by binding directly to collagen and subsequently causing platelet aggregation. The aim of this study was to identify the active region of AAPP responsible for the anti-thrombotic activity because we hypothesized that AAPP could be used as a candidate anti-platelet drug. We identified that the essential moiety of AAPP for collagen binding and anti-thrombotic activity was in the region encoded by exon 3-4. The crystal structure of this domain was also determined. AAPP inhibited whole blood aggregation induced by collagen at 10 mg/kg body weight. AAPP prevented pulmonary death at a lower dose (3 mg/kg) without prolongation of bleeding time compared with aspirin (100 mg/kg) that compromised hemostasis. These results indicate that AAPP has great potential as a new anti-platelet agent with a better risk/benefit ratio than that seen with aspirin.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (16 results)

All 2014 2013 2012

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 1 results) Presentation (9 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] The crystal structure of the active domain of Anopheles anti-platelet protein, a powerful anti-coagulant, in complex with an antibody2014

    • Author(s)
      Sugiyama K, Iyori M, Sawaguchi A, Akashi S, Tame JR, Park SY, Yoshida S.
    • Journal Title

      J Biol Chem

      Volume: (in press)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The crystal structure of the active domain of Anopheles anti-platelet protein, a powerful anti-coagulant, in complex with an antibody.2014

    • Author(s)
      Sugiyama, K., Iyori, M., Sawaguchi, A., Akashi, S., Tame, J.R.H., Park, S.-Y., Yoshida, S.
    • Journal Title

      J. Biol. Chem.

      Volume: 289 Issue: 23 Pages: 16303-16312

    • DOI

      10.1074/jbc.m114.564526

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Visualization and live imaging analysis of a mosquito saliva protein in host animal skin using a transgenic mosquito with a secreted luciferase reporter system2013

    • Author(s)
      Yamamoto D, Yokomine T, Sumitani M, Yagi K, Matsuoka H, Yoshida S.
    • Journal Title

      Insect Mol Biol

      Volume: 22 Pages: 685-93

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reduction of malaria transmission by transgenic mosquitoes expressing an anti-sporozoite antibody in their salivary glands2013

    • Author(s)
      Sumitani M, Kasashima K, Yamamoto DS, Yagi K, Yuda M, Matsuoka H, Yoshida S.
    • Journal Title

      Insect Mol Biol

      Volume: 22 Pages: 41-51

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of the active region responsible for the anti-thrombotic activity of anopheline anti-platelet protein from a malaria vector mosquito2013

    • Author(s)
      Hayashi H, Kyushiki H, Nagano K, Sudo T, Iyori M, Matsuoka H, Yoshida S.
    • Journal Title

      Platelets

      Volume: 24 Pages: 324-332

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Reduction of malaria transmission by transgenic mosquitoes expressing an anti-sporozoite antibody in their salivary glands2013

    • Author(s)
      Sumitani M
    • Journal Title

      Insect Mol Biol

      Volume: in press Issue: 1 Pages: 1-11

    • DOI

      10.1111/j.1365-2583.2012.01168.x

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] インドネシア人における蚊唾液タンパクに対する抗体価とマラリア感染2014

    • Author(s)
      伊従光洋,藤吉里紗,舟津知宏,澤口明日香,Din Syafruddin,吉田栄人
    • Organizer
      第66回日本衛生動物学会大会
    • Place of Presentation
      岐阜大学(岐阜)
    • Related Report
      2013 Final Research Report
  • [Presentation] インドネシア人における蚊唾液タンパクに対する抗体価とマラリア感染2014

    • Author(s)
      伊従光洋,藤吉里紗,舟津知宏,澤口明日香,Din Syafruddin,吉田栄人
    • Organizer
      第66回日本衛生動物学会大会
    • Place of Presentation
      岐阜大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 「Vector-borne diseases研究 : ラボからフィールドへ」トランスジェニック蚊を用いたマラリア原虫−唾液腺相互作用の解明2013

    • Author(s)
      吉田栄人
    • Organizer
      日本獣医寄生虫学会シンポジウム
    • Place of Presentation
      東京大学駒場キャンパス(東京)
    • Year and Date
      2013-03-28
    • Related Report
      2013 Final Research Report
  • [Presentation] 2種の蚊由来唾液タンパクにおける生物学的機能比較2013

    • Author(s)
      伊従光洋,澤口明日香,吉田栄人
    • Organizer
      第70回日本衛生動物学会西日本支部大会
    • Place of Presentation
      越前市福祉健康センター(越前市)
    • Related Report
      2013 Final Research Report
  • [Presentation] Genetic modification (GM)ハマダラカの唾液腺内のマラリア原虫の動態のin vivoイメージング2013

    • Author(s)
      吉田栄人
    • Organizer
      第69回日本寄生虫学会西日本支部大会
    • Place of Presentation
      穴吹ホール(高松)
    • Related Report
      2013 Final Research Report
  • [Presentation] ハマダラカ唾液タンパクAAPPに対する抗体の特性2013

    • Author(s)
      伊従光洋、藤吉里紗、澤口明日香、吉田栄人
    • Organizer
      第54回日本熱帯医学会大会
    • Place of Presentation
      長崎パブリックホール(長崎)
    • Related Report
      2013 Final Research Report
  • [Presentation] 2種の蚊由来唾液タンパクにおける生物学的機能比較2013

    • Author(s)
      伊従光洋,澤口明日香,吉田栄人
    • Organizer
      第68回日本衛生動物学会西日本支部大会
    • Place of Presentation
      越前市福祉健康センター
    • Related Report
      2013 Annual Research Report
  • [Presentation] 抗マラリアスポロゾイト抗体を唾液腺で発現する遺伝子組換えハマダラカはマラリア伝播能を喪失する2012

    • Author(s)
      炭谷めぐみ、笠島克巳、八木啓太、山本大介、油田正夫、松岡裕之、吉田栄人
    • Organizer
      第30回北陸病害動物研究会
    • Place of Presentation
      三国文化未来館ホール(板井市)
    • Year and Date
      2012-06-30
    • Related Report
      2013 Final Research Report
  • [Presentation] Salivary glands as vaccine delivery target2012

    • Author(s)
      Yoshida S, Yamamoto D, Sumitani, M, Matsuoka H.
    • Organizer
      XXIV International Congress of Entomology (ICE 2012)
    • Place of Presentation
      Exco-Daegu Exhibition Hall (Korea, Degue)
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] Anopheline Anti-Platelet Proteinを認識する抗体又は抗体断片2013

    • Inventor(s)
      吉田栄人
    • Industrial Property Rights Holder
      国立大学法人金沢大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-031891
    • Filing Date
      2013-02-21
    • Related Report
      2013 Final Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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