| Project/Area Number |
24659472
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
膠原病・アレルギー・感染症内科学
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
HARIGAI Masayoshi 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (20238207)
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| Co-Investigator(Kenkyū-buntansha) |
NANKI Toshihiro 帝京大学, 医学部, 准教授 (00282749)
MIYASAKA Nobuyuki 東京医科歯科大学, 医学部, 非常勤講師 (30157622)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 膠原病学 / 抗好中球細胞質抗体関連血管炎 / 関節リウマチ / NETS形成 / シトルリン化 / 自然免疫 |
|---|
| Research Abstract |
Objective: We investigated involvement of neutrophil extracellular traps (NETs) formation in pathogenesis of anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis and tried to develop a murine model of systemic vasculitis. Methods: We separated neutrophils from peripheral blood using a magnet beads-based negative selection method. Immunoglobulin G (IgG) fraction of Myeloperoxidase (MPO)-ANCA positive serum was prepared using Protein A column. A murine model of systemic vasculitis was explored using Candida albicans water-soluble fraction (CAWS) and house-made rabbit anti-mouse MPO polyclonal antibody. Results. IgG fraction of MPO-ANCA positive serum induced NETs formation in the presence of recombinant C5a. CAWS and house-made rabbit anti-mouse MPO polyclonal antibody could not induce systemic vasculitis in C57BL6 mice. We measured panel of cytokines and chemokines in sera from patients with ANCA-associated vasculitis. All cytokines and chemokines were measurable in them.
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