| Project/Area Number |
24659475
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
膠原病・アレルギー・感染症内科学
|
|---|
| Research Institution | Institute of Physical and Chemical Research (2013)
Kanazawa Medical University (2012) |
|---|
Principal Investigator |
ASANO Yoshihiro 独立行政法人理化学研究所, 統合生命医科学研究センター, 客員研究員 (70114353)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MARUYAMA Saho 愛媛大学, 医学系研究科, 助教 (10301326)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
TOMOSUGI Naohisa 金沢医科大学, 総合医学研究所, 教授 (80155580)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | アレルギー / 転写因子IRF-1 / IL-4 / Th2免疫応答 |
|---|
| Research Abstract |
We focused on the role of IRF-1 during a pathogen infection, especially the involvement of IRF-1 in the suppression of Th2 during the pathogen infection. We found that IRF-1 directly suppresses the transcription of Il4 gene of Th2 cells. IRF-1 binds to the silencer region of Il4 gene by the influence of soluble factor that produced by Listeria-infected APCs and down-regulates the Il4 gene transcription. We identified that IL-1 mediates the activity of the soluble factor. According to the results presented, we propose that IRF-1 functions to induce the Th1 shift via two distinct mechanisms, the previously known mechanism and a novel function described here. IRF-1 activates the Il12 p40 gene promoter activity and induces the gene transcription, resulted in the shift to Th1 subset. In addition, IRF-1 acts on the silencer region of Il4 gene by the influence of IL-1 and inhibits the transcription of the Il4 gene in Th2 cells.
|
