Infection kinetics of avian H5N1 influenza viruses in human airway epithelial cells by bio-imaging analysis

• Project/Area Number: 24659482
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Infectious disease medicine
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: NAKAYA Takaaki 京都府立医科大学, 医学(系)研究科(研究院), 教授 (80271633)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: インフルエンザウイルス / H5N1 / 呼吸器上皮細胞 / バイオイメージング / ウイルス

Research Abstract

Highly pathogenic avian influenza H5N1 viruses have spread across Eurasia and into Africa; however, the mechanism underlying the pathogenesis of H5N1 in humans remains unclear. To identify differences in the mechanisms underlying the tropism of current H5N1 and previous avian influenza virus strains for human lower respiratory tract epithelial cells, we analyzed the infection kinetics of both viruses in epithelial cells using bio-imaging techniques. The results suggest that the pH of endosomes correlates closely with susceptibility to viruses, and that infectivity is dependent on the stability of viral hemagglutinin protein in acidic endosomes.

Research Products

• [Journal Article] The lipid mediator protectin D1 inhibits influenza virus replication and improves severe influenza (2013)
  • Author(s): Morita M, Kuba K, Ichikawa A, Nakayama M, Katahira J, Iwamoto R, Watanebe T, Sakabe S, Daidoji T, Nakamura S, Kadowaki A, Ohto T, Nakanishi H, Taguchi R, Nakaya T, Murakami M, Yoneda Y, Arai H, Kawaoka Y, Penninger JM, Arita M, Imai Y.
  • Journal Title: Cell Volume: 153 Issue: 1 Pages: 112
  • DOI: 10.1016/j.cell.2013.02.027
  • URL: https://localhost/en/publications/6ee738d0-ec30-4874-be2a-611187484000
  • Peer Reviewed
• [Journal Article] 鳥インフルエンザ (2013)
  • Author(s): 中屋隆明
  • Journal Title: Neuroinfection Volume: 18(1) Pages: 28-33
• [Journal Article] インフルエンザウイルスHAタンパク質の病原性分子機構 (2013)
  • Author(s): 中屋隆明
  • Journal Title: 京府医大誌 Volume: 122(3) Pages: 123-131
  • NAID: 40019652403
• [Journal Article] Human monoclonal antibodies broadly neutralizing against influenza B virus. (2013)
  • Author(s): Yasugi, M., Kubota-Koketsu, R., Yamashita, A., Kawashita, N., Du, A., Sasaki, T., Nishimura, M., Misaki, R., Kuhara, M., Boonsathorn, N., Fujiyama, K., Okuno, Y., Nakaya, T., and Ikuta, K.
  • Journal Title: PLoS Pathog. Volume: 9 Issue: 2 Pages: e1003150-e1003150
  • DOI: 10.1371/journal.ppat.1003150
  • Peer Reviewed
• [Journal Article] Antigenic analysis of highly pathogenic avian influenza virus H5N1 sublineage co-circulating in Egypt. (2012)
  • Author(s): Watanabe, Y., Ibrahim, M.S., Ellakany, H.F., Kawashita, N., Daidoji, T., Takagi, T., Yasunaga, T., Nakaya, T., and Ikuta, K.
  • Journal Title: J. Gen. Virol. Volume: 93 Issue: 10 Pages: 2215-2226
  • DOI: 10.1099/vir.0.044032-0
  • Peer Reviewed
• [Journal Article] Emergence of HA mutants during influenza virus pneumonia (2012)
  • Author(s): Manriquez ME, Nakaya T, Shinya K, et al.
  • Journal Title: Int. J. Clin. Exp. Pathol. Volume: 5(8) Pages: 787-795
  • Peer Reviewed
• [Presentation] 鳥インフルエンザウイルスのヒト呼吸器上皮細胞に対する感染メカニズム (2013)
  • Author(s): 大道寺智、渡邊洋平、Madiha S. Ibrahim、安木真世、丸山央峰、益田泰輔、新井史人、大場誠介、本田文江、生田和良、中屋隆明
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸市(ミニシンポジウム)
  • Year and Date: 2013-11-12
• [Presentation] エジプトにおける患者由来H5N1高病原性鳥インフルエンザウイルスHA遺伝子の変異解析 (2013)
  • Author(s): 渡邊洋平、Madiha S. Ibrahim、大道寺智、荒井泰葉、平松宏明、中屋隆明、鈴木康夫、生田和良
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸市(一般口演)
  • Year and Date: 2013-11-12
• [Presentation] Critical role of hemagglutinin (HA) in pathogenesis of influenza A viruses (2013)
  • Author(s): 中屋隆明
  • Organizer: 日露国際ワークショップ
  • Place of Presentation: (京都大学)京都市
  • Year and Date: 2013-10-30
• [Presentation] 血液感染症の治療戦略 (2013)
  • Author(s): 中屋隆明
  • Organizer: 京滋血液感染症研究会2013
  • Place of Presentation: 京都市(特別講演)
  • Year and Date: 2013-09-13
• [Presentation] 鳥インフルエンザ (2012)
  • Author(s): 中屋隆明
  • Organizer: 日本神経感染症学会
  • Place of Presentation: 京都市(教育講演)
  • Year and Date: 2012-10-20
• [Presentation] インフルエンザウイルスの宿主域拡大戦略 (2012)
  • Author(s): 中屋隆明
  • Organizer: CREST公開シンポジウム「光が拓く細胞解析の新展開」
  • Place of Presentation: 東京(品川)
  • Year and Date: 2012-08-18
  • Invited
• [Presentation] 新興・再興ウイルス感染症のダイナミクス (2012)
  • Author(s): 中屋隆明
  • Organizer: びわ湖国際医療フォーラム
  • Place of Presentation: 滋賀県大津市(特別講演)
  • Year and Date: 2012-07-14
• [Presentation] 鳥インフルエンザウイルスのヒト呼吸器上皮細胞に対する感染メカニズム
  • Author(s): 大道寺 智
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸国際会議場（神戸市）
• [Presentation] Critical role of hemagglutinin (HA) in pathogenesis of influenza A viruses
  • Author(s): 中屋 隆明
  • Organizer: 2013日露ワークショップ
  • Place of Presentation: 京都大学（京都市）
  • Invited
• [Presentation] H1N1 2009-HAゲノムのディープシークエンス解析
  • Author(s): 中屋 隆明
  • Organizer: インフルエンザ研究者交流の会シンポジウム
  • Place of Presentation: 福島県耶麻郡
• [Presentation] 新興・再興ウイルス感染症のダイナミクス
  • Author(s): 中屋 隆明
  • Organizer: びわ湖国際医療フォーラム
  • Place of Presentation: 滋賀県大津市
  • Invited
• [Presentation] インフルエンザウイルスの宿主域拡大戦略
  • Author(s): 中屋 隆明
  • Organizer: CREST公開シンポジウム「光が拓く細胞解析の新展開」
  • Place of Presentation: 東京都品川区
• [Presentation] 鳥インフルエンザ
  • Author(s): 中屋 隆明
  • Organizer: 日本神経感染症学会
  • Place of Presentation: 京都府京都市
  • Invited
• [Remarks] ホームページURL
  • URL: http://www.f.kpu-m.ac.jp/k/did/
• [Remarks] ホームページURL
  • URL: http://www.kpu-m.ac.jp/doc/classes/basicmedicine/221.html