Infection kinetics of avian H5N1 influenza viruses in human airway epithelial cells by bio-imaging analysis
| Project/Area Number |
24659482
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Infectious disease medicine
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
NAKAYA Takaaki 京都府立医科大学, 医学(系)研究科(研究院), 教授 (80271633)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | インフルエンザウイルス / H5N1 / 呼吸器上皮細胞 / バイオイメージング / ウイルス |
|---|
| Research Abstract |
Highly pathogenic avian influenza H5N1 viruses have spread across Eurasia and into Africa; however, the mechanism underlying the pathogenesis of H5N1 in humans remains unclear. To identify differences in the mechanisms underlying the tropism of current H5N1 and previous avian influenza virus strains for human lower respiratory tract epithelial cells, we analyzed the infection kinetics of both viruses in epithelial cells using bio-imaging techniques. The results suggest that the pH of endosomes correlates closely with susceptibility to viruses, and that infectivity is dependent on the stability of viral hemagglutinin protein in acidic endosomes.
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Report
(3 results)
Research Products
(21 results)
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[Journal Article] The lipid mediator protectin D1 inhibits influenza virus replication and improves severe influenza2013
Author(s)
Morita M, Kuba K, Ichikawa A, Nakayama M, Katahira J, Iwamoto R, Watanebe T, Sakabe S, Daidoji T, Nakamura S, Kadowaki A, Ohto T, Nakanishi H, Taguchi R, Nakaya T, Murakami M, Yoneda Y, Arai H, Kawaoka Y, Penninger JM, Arita M, Imai Y.
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Journal Title
Cell
Volume: 153
Issue: 1
Pages: 112-125
DOI
Related Report
Peer Reviewed
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[Journal Article] Human monoclonal antibodies broadly neutralizing against influenza B virus.2013
Author(s)
Yasugi, M., Kubota-Koketsu, R., Yamashita, A., Kawashita, N., Du, A., Sasaki, T., Nishimura, M., Misaki, R., Kuhara, M., Boonsathorn, N., Fujiyama, K., Okuno, Y., Nakaya, T., and Ikuta, K.
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Journal Title
PLoS Pathog.
Volume: 9
Issue: 2
Pages: e1003150-e1003150
DOI
Related Report
Peer Reviewed
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[Journal Article] Antigenic analysis of highly pathogenic avian influenza virus H5N1 sublineage co-circulating in Egypt.2012
Author(s)
Watanabe, Y., Ibrahim, M.S., Ellakany, H.F., Kawashita, N., Daidoji, T., Takagi, T., Yasunaga, T., Nakaya, T., and Ikuta, K.
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Journal Title
J. Gen. Virol.
Volume: 93
Issue: 10
Pages: 2215-2226
DOI
Related Report
Peer Reviewed
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