Hematopoietic stem cell potential is propagated by human pluripotent stem cell-derived endothelial stroma

• Project/Area Number: 24659496
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Pediatrics
• Research Institution: Kyoto University
• Principal Investigator: NAKAHATA Tatsutoshi 京都大学, iPS細胞研究所, 教授 (20110744)
• Co-Investigator(Renkei-kenkyūsha): NIWA Akira 京都大学, ips細胞研究所, 助教 (20546999) ; OSHIMA Kohichi 京都大学, ips細胞研究所, 研究員 (60525377)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: ES細胞 / iPS細胞 / 造血幹細胞 / ストロマ細胞 / ニッチ

Research Abstract

Elucidating the mechanism of homeostasis in hematopoietic system and developing the culture to expand hematopoietic stem cells are of great interest in medical scientific fields. We developed the method to effectively and robustly induce VE-Cadherin+ endothelial stroma cells from human pluripotent stem cells. Those stromal cells can increase CFU in cord blood CD34+ hematopoietic stem/progenitor fraction by two to three times. Moreover, serial transplantation studies with NOG mice indicated that two-week co-culture can double the Scid-repopulating cells.

Research Products

• [Journal Article] Genetic correction of HAX1 in induced pluripotent stem cells from a patient with severe congenital neutropenia improves defective granulopoiesis (2014)
  • Author(s): Morishima T., Watanabe K., Niwa A., Hirai H., Saida S., Tanaka T., Kato I., Umeda K., Hiramatsu H., Saito M. K., Matsubara K., Adachi S., Kobayashi M., Nakahata T., Heike T.
  • Journal Title: Haematologica Volume: 99(1) Pages: 19-27
• [Journal Article] HAX1 in induced pluripotent stem cells from a patient with severe congenital neutropenia improves defective granulopoiesis. (2014)
  • Author(s): Morishima T, Watanabe K, Niwa A, Hirai H, Saida S, Tanaka T, Kato I, Umeda K, Hiramatsu H, Saito MK, Matsubara K, Adachi S, Kobayashi M, Nakahata T, Heike T
  • Journal Title: Haematologica Volume: 99(1) Issue: 1 Pages: 19-27
  • DOI: 10.3324/haematol.2013.083873
  • Peer Reviewed / Open Access
• [Journal Article] PBSC collection from family donors in Japan : a prospective survey (2014)
  • Author(s): Kodera Y, Yamamoto K, Harada M, Morishima Y, Dohy H, Asano S, Ikeda Y, Nakahata T, Imamura M, Kawa K, Kato S, Tanimoto M, Kanda Y, Tanosaki R, Shiobara S, Kim SW, Nagafuji K, Hino M, Miyamura K, Suzuki R, Hamajima N, Fukushima M, Tamakoshi A
  • Journal Title: Bone Marrow Transplant Volume: 49 Issue: 2 Pages: 195-200
  • DOI: 10.1038/bmt.2013.147
  • Peer Reviewed
• [Journal Article] The clinical utility of genetic testing for t(8;16)(p11;p13) in congenital acute myeloid leukemia. (2014)
  • Author(s): Daifu T., Kato I., Kozuki K., Umeda K., Hiramatsu H., Watanabe K., Kamiya I., Taki T., Nakahata T., Heike T., Adachi S.
  • Journal Title: J. Pediatr. Hematol/Onc. Volume: in press
  • Peer Reviewed
• [Journal Article] Clinical characteristics of 15 children with juvenile myelomonocytic leukaemia who developed blast crisis: MDS Committee of Japanese Society of Paediatric Haematology/Oncology. (2014)
  • Author(s): Honda Y, Tsuchida M, Zaike Y, Masunaga A, Yoshimi A, Kojima S, Ito M, Kikuchi A, Nakahata T, Manabe A.
  • Journal Title: Br J Haematol Volume: in press Issue: 5 Pages: 682-687
  • DOI: 10.1111/bjh.12796
  • Peer Reviewed
• [Journal Article] Appropriate dose reduction in induction therapy is essential for the treatment of infants with acute myeloid leukemia: a report from the Japanese Pediatric Leukemia/Lymphoma Study Group. (2013)
  • Author(s): Tomizawa D, Tawa A, Watanabe T, Saito AM, Kudo K, Taga T, Iwamoto S, Shimada A, Terui K, Moritake H, Kinoshita A, Takahashi H, Nakayama H, Kiyokawa N, Isoyama, K, Mizutani S, Hara J, Horibe K, Nakahata T, Adachi S.
  • Journal Title: Int J Hematol Volume: 98 Issue: 5 Pages: 578-588
  • DOI: 10.1007/s12185-013-1429-2
  • Peer Reviewed
• [Journal Article] for the Japan Society for Hematopoietic Cell Transplantation, Halter J., Schmitz N., Niederwieser D., Gratwohl A. PBSC collection from family donors in Japan : a prospective survey (2013)
  • Author(s): Kodera Y., Yamamoto K., Harada M., Morishima Y, Dohy H, Asano S, Ikeda Y., Nakahata T., Imamura M., Kawa K., Kato S., Tanimoto M., Kanda Y., Tanosaki R., Shiobara S., Kim SW., Nagafuji K., Hino M., Miyamura K.,Suzuki R., Hama j ima N., Fukushima M., Tamakoshi A.
  • Journal Title: Bone Marrow Transplant
• [Journal Article] Excess treatment reduction including anthracyclines results in higher incidence of relapse in core binding factor acute myeloid leukemia in children. (2013)
  • Author(s): Tomizawa D, Tawa A, Watanabe T, Moriya-Saito A, Kudo K, Taga T, Iwamoto S, Shimada A, , Terui K, Moritake H, Kinoshita A, Takahashi H, Nakayama H, Koh K, Kigasawa H, Kosaka Y, Miyachi H, Horibe K, Nakahata T, and Adachi S.
  • Journal Title: Leukemia Volume: 27 Issue: 12 Pages: 2413-2416
  • DOI: 10.1038/leu.2013.153
  • Peer Reviewed
• [Journal Article] Robust and highly-efficient differentiation of functional monocytic cells from human pluripotent stem cells under serum- and feeder cell- free conditions. (2013)
  • Author(s): Yanagimachi MD, Niwa A, Tanaka T, Ozaki F, Nishimoto S, Murata Y, Yasumi T, Ito J, Tomida S, Oshima K, Asaka I, Goto H, Heike T, Nakahata T, Saito MK
  • Journal Title: PLoS ONE Volume: 8(4) Issue: 4 Pages: e59243-e59243
  • DOI: 10.1371/journal.pone.0059243
  • NAID: 120005244458
  • Peer Reviewed
• [Journal Article] Clonal selection in xenografted TAM recapitulates the evolutionary process of myeloid leukemia in Down syndrome. (2013)
  • Author(s): Saida, S., K. Watanabe, A. Sato-Otsubo, K. Terui, K. Yoshida, Y. Okuno, T. Toki, R. Wang, Y. Shiraishi, S. Miyano, I. Kato, T. Morishima, H. Fujino, K. Umeda, H. Hiramatsu, S. Adachi, E. Ito, S. Ogawa, M. Ito, T. Nakahata, and T. Heike.
  • Journal Title: Blood Volume: 121 Issue: 21 Pages: 4377-4387
  • DOI: 10.1182/blood-2012-12-474387
  • Peer Reviewed
• [Journal Article] Recipient seropositivity for adenovirus type 11 is a highly predictive factor for the development of AdV11-induced hemorrhagic cystitis after allogeneic hematopoietic SCT. (2013)
  • Author(s): Nakazawa Y., Saito S., Yanagisawa R., Suzuki T., Toshiro Ito T., Ishida F., Muramatsu H., Matsumoto K., Kato K., Ishida H., Umeda K., Souichi Adachi S., Nakahata T., Koike K.
  • Journal Title: Bone Marrow Transplant. Volume: 48 Issue: 5 Pages: 737-739
  • DOI: 10.1038/bmt.2012.206
  • Peer Reviewed
• [Journal Article] 疾患特異的ips細胞 (2013)
  • Author(s): 斎藤潤、中畑龍俊
  • Journal Title: 再生医療 Volume: 12(1) Pages: 19-29
  • NAID: 130000450684
• [Journal Article] Recipient seropositivity for adenovirus type 11 is highly predictive of the development of hemorrhagic cystitis after allogeneic hematopoietic stem cell transplantation. (2013)
  • Author(s): Nakazawa Y., Saito S., Yanagisawa R., Suzuki T., Toshiro Ito T., Ishida F., Muramatsu H., Matsumoto K., Kato K., Ishida H., Umeda K., Souichi Adachi S., Nakahata T., Koike K.
  • Journal Title: Bone Marrow Transplant. Volume: in press
  • Peer Reviewed
• [Journal Article] Clonal selection in xenografted TAM recapitulatesthe evolutionary process of myeloid leukemia in Down syndrome. (2013)
  • Author(s): Saida S., Watanabe K., Wang RN., Shiraishi Y., Miyano S., Kato I., Morishima T., Fujino H., Umeda K., Hiramatsu H., Adachi S., Ito E., Ogawa S., Ito M., Nakahata T., Heike T.他５名２０番目
  • Journal Title: Blood Volume: in press
  • Peer Reviewed
• [Journal Article] 疾患特異的iPS細胞 (2013)
  • Author(s): 斎藤潤、中畑龍俊
  • Journal Title: 再生医療 Volume: 12 Pages: 19-29
  • NAID: 130000450684
• [Journal Article] The CD40-CD40L axis and IFN-γ play critical roles in Langhans giant cell formation (2012)
  • Author(s): Sakai H, Okafuji I, Nishikomori R, Abe J, Izawa K, Kambe N, Y asumi T, Nakahata T, Heike T
  • Journal Title: Int Immunol Volume: 24 Issue: 1 Pages: 5-15
  • DOI: 10.1093/intimm/dxr088
  • Peer Reviewed
• [Journal Article] Detection of Base Substitution-Type Somatic Mosaicism of the NLRP3 Gene with> 99. 9% Statistical Confidence by Massively Parallel Sequencing (2012)
  • Author(s): Izawa, K., A. Hijikata, N. Tanaka, T. Kawai, M. K. Saito, R. Goldbach-Mansky, I. Aksentijevich, T. Yasumi, T. Nakahata, T. Heike, R. Nishikomori, and O. Ohara
  • Journal Title: DNA Res Volume: 19 Issue: 2 Pages: 143-52
  • DOI: 10.1093/dnares/dsr047
  • Peer Reviewed
• [Journal Article] Frequent somatic mosaicism of NEMO in T cells of patients with X-linked anhidrotic ectodermal dysplasia with immune- deficiency (2012)
  • Author(s): Kawai T, Imai C et al
  • Journal Title: Blood Volume: 119 Issue: 23 Pages: 5458-66
  • DOI: 10.1182/blood-2011-05-354167
  • Peer Reviewed
• [Journal Article] Dominant-negative STAT1 SH2 domain mutations in unrelated patients with Mendelian susceptibility to mycobacterial disease. (2012)
  • Author(s): Tsumura M.
  • Journal Title: Human Mutation Human Mutation Volume: 33 Issue: 9 Pages: 1377-1387
  • DOI: 10.1002/humu.22113
  • Peer Reviewed
• [Journal Article] Outcome of children with Refractory Anaemia with Excess of Blast (RAEB) and RAEB in Transformation (RAEB-T) in the Japanese MDS99 study. (2012)
  • Author(s): Kikuchi A.
  • Journal Title: Brit. J. Haematol. Volume: 158 Issue: 5 Pages: 657-661
  • DOI: 10.1111/j.1365-2141.2012.09210.x
  • Peer Reviewed
• [Journal Article] Induced pluripotent stem cells from CINCA syndrome patients as a model for dissecting somatic mosaicism and drug discovery (2012)
  • Author(s): Tanaka T., Takahashi K., Yamane M., Tomida S., Nakamura S., Oshima K., Niwa A., Nishikomori R., Kambe N., Hara H., Mitsuyama M., Morone N., Heuse J.E., Yamamoto T., Watanabe A., Sato-Otsubo A., Ozawa S., Asaka I., Heike T., Yamanaka S., Nak
  • Journal Title: Blood Volume: 120(6) Issue: 6 Pages: 1299-308
  • DOI: 10.1182/blood-2012-03-417881
  • Peer Reviewed
• [Journal Article] Multiple reversions of an IL2RG mutation restore combined immunodeficiency patient. (2012)
  • Author(s): Kawai T., Saito M., Nishikomori R., Yasumi T., Izawa K., Murakami T., Okamoto N., Mori Y., Nakagawa N., Imai K., Nonoyama S., Wada T., Yatie A., Oomori K., Nakahata T., Heike T.
  • Journal Title: J. Clin. Immunol Volume: 32(4) Issue: 4 Pages: 690-7
  • DOI: 10.1007/s10875-012-9684-1
  • Peer Reviewed
• [Journal Article] 白血病治療の進歩と今後の展望.日本小児 (2012)
  • Author(s): 中畑龍俊
  • Journal Title: 血液・がん学会雑誌 Volume: 第49巻1・2号 Pages: 11-15
• [Journal Article] 幹細胞増幅、第10章内科疾患と再生医療 (2012)
  • Author(s): 中畑龍俊、丹羽明
  • Journal Title: カラー版内科学、門脇孝、永井良三(総編集) Pages: 447-450
• [Journal Article] The CD40-CD40L axis and INF-g play critical roles in Langerhans giant cell formation. (2012)
  • Author(s): Sakai H., Okafuji I., Nishikomori R., Abe J., Izawa K., Kambe N., Yasumi T., Nakahata T., Heike T.
  • Journal Title: Int. Immunol. Volume: 24 Pages: 5-15
  • Peer Reviewed
• [Journal Article] Detection of base substitution–type somatic mosaicism of the NLRP3 gene with >99.9% statistical confidence by massively parallel sequencing. (2012)
  • Author(s): IzawaK., Hijikata A., Tanaka N., Kawai T., Saito M.K., Goldbach-Mansky R., Aksentijevich I., Yasumi T., Nakahata T., Heike T., Nishikomori R., OharaO.
  • Journal Title: DNA Res. Volume: 19 Pages: 143-152
  • Peer Reviewed
• [Journal Article] Pediatric idiopathic TTP diagnosed with decreased ADAMTS13 activity. (2012)
  • Author(s): Morishima T., Nomura A., Saida S., Watanabe K., Yagi H., Matsumoto M., Fujimura Y., Heike T., Nakahata T., Adachi S.
  • Journal Title: Pediatr. Int. Volume: 54 Pages: 422-423
  • Peer Reviewed
• [Journal Article] Frequent somatic mosaicism of NEMO in T cells of patients with X-linked anhidrotic ectodermal dysplasia and immunodeficiency. (2012)
  • Author(s): Kawai T., Nishikomori R.,Saito M., Yasumi T., Takaoka Y., Nakahata T., Sasaki S., Ito E., Akutagawa H., Kawai T., Imai C., Okada S., Kobayashi M., Heike T.他９名１０番目
  • Journal Title: Blood Volume: 119 Pages: 5458-5466
  • Peer Reviewed
• [Journal Article] Induced pluripotent stem cells from CINCA syndrome patients as a model for dissecting somatic mosaicism and drug discovery. (2012)
  • Author(s): Tanaka T., Oshima K., Niwa A., Nishikomori R., Kambe N., Hara H., Mitsuyama M., Ozawa S., Asaka I., Heike T., Yamanaka S., Nakahata T., Saito M.K. 他７名１５番目
  • Journal Title: Blood Volume: 120 Pages: 1299-1308
  • Peer Reviewed
• [Journal Article] Multiple reversions of an IL2RG mutation restore combined immunodeficiency patient. (2012)
  • Author(s): Kawai T., Saito M., Nishikomori R., Yasumi T., Izawa K., Murakami T., Okamoto N., Mori Y., Nakagawa N., Imai K., Nonoyama S., Wada T., Yatie A., Oomori K., Nakahata T., Heike T.
  • Journal Title: J. Clin. Immunol. Volume: 32 Pages: 690-697
  • Peer Reviewed
• [Journal Article] 白血病治療の進歩と今後の展望 (2012)
  • Author(s): 中畑龍俊
  • Journal Title: 日本小児血液・がん学会雑誌 Volume: 49 Pages: 11-15
• [Journal Article] 幹細胞増幅 (2012)
  • Author(s): 中畑龍俊、丹羽明
  • Journal Title: カラー版内科学 Volume: 総編集
• [Journal Article] Clinical characteristics of 15 children with juvenile myelomonocytic 1 leukemia who developed blast crisis : MDS Committee of Japanese Society of Pediatric Hematology/Oncology (JSPHO)
  • Author(s): Honda Y., Tsuchida M., , Zaike Y., Masunaga A., Yoshimi A., Kojima S., Ito M., Kikuchi A., Nakahata T., Manabe A.
  • Journal Title: Brit. J. Haematol Volume: (In press)
• [Journal Article] The clinical utility of genetic testing for t(8;16) (p11;p13) in congenital acute myeloid leukemia
  • Author(s): Daifu T., Kato I., Kozuki K., Umeda K., Hiramatsu H., Watanabe K., Kamiya I., Taki T., Nakahata T., Heike T., Adachi S.
  • Journal Title: J. Pediatr. Hematol/Onc Volume: (In press)
• [Presentation] ips細胞の小児医療への応用 (2013)
  • Author(s): 中畑龍俊
  • Organizer: 第38回東日本小児科学会
  • Place of Presentation: 大宮ソニックシティ
  • Year and Date: 2013-11-23
• [Presentation] ips細胞を活用した医療の可能性と倫理 (2013)
  • Author(s): 中畑龍俊
  • Organizer: 第10回STSフォーラム「科学技術が拓く人間の未来」公開シンポジウム
  • Place of Presentation: 京都商工会議所ビル講堂
  • Year and Date: 2013-10-05
• [Presentation] さい帯血造血幹細胞発見秘話とips細胞ストックの月齊帯 (2013)
  • Author(s): 中畑龍俊
  • Organizer: 血活用の未来像.さい帯血移植1万例突破記念事業「さらなる飛躍へのステップ」記念講演会
  • Place of Presentation: TKP田町カンファレンスセンター
  • Year and Date: 2013-09-28
• [Presentation] ips細胞研究が切り開く未来の医療 (2013)
  • Author(s): 中畑龍俊
  • Organizer: 日本学術会議公開学術講演会「未来社会を築く生命科学と医療のフロンティア」
  • Place of Presentation: 京都大学薬学部記念講堂
  • Year and Date: 2013-08-03
• [Presentation] ips細胞の臨床応用 (2013)
  • Author(s): 中畑龍俊
  • Organizer: 第55回日本小児」血液・がん学会学術集会
  • Place of Presentation: ヒルトン福岡シーホーク
• [Presentation] Xenograft model of TAM reveals the evolutionary process of myeloid leukemia in Down syndrome (2013)
  • Author(s): Saida S., Watanabe K., Sato-Otsubo A., Terui K., Yoshida K., Okuno Y., Toki T., Wang R., Shiraishi Y., Miyano S., Kato I., Umeda K., Hiramatsu H., Fu j ino H., Adachi S., Nakahata T., Ito E., Ogawa S., Heike T.
  • Organizer: 第75回日本血液学会学術集会
  • Place of Presentation: 札幌市教育分化会館(一般口演)
• [Presentation] Mesodermal development from reprogrammed Fanconi anemia cells is affected by ALDH2 enzymatic activity (2013)
  • Author(s): Suzuki N., Hira A., Niwa A., Matsuo K., Takata M., Yabe M., Nakahata T., Saito M.
  • Organizer: 11th Annual Meeting of international Society for Stem Cell Research (ISSCR)
  • Place of Presentation: Boston, MA, USA
• [Presentation] iPS細胞を用いた今後の医療の可能性 (2013)
  • Author(s): 中畑龍俊
  • Organizer: 平成25年度神奈川県内科医学会総会
  • Place of Presentation: 神奈川県総合医療会館
  • Invited
• [Presentation] 罹患者由来iPS細胞を用いたCINCA症候群における関節病態の分子機構の解明 (2013)
  • Author(s): 横山宏司、西小森隆太、池谷真、那須輝、田中孝之、齋藤潤、梅田雄嗣、中畑龍俊、戸口田淳也、平家俊男
  • Organizer: 第34回日本炎症・再生医学会
  • Place of Presentation: 国立京都国際会館
• [Presentation] Mesodermal development from reprogrammed Fanconi anemia cells is affected by ALDH2 enzymatic activity. (2013)
  • Author(s): Suzuki N., Hira A., Niwa A., Matsuo K., Takata M., Yabe M., Nakahata T., Saito M.
  • Organizer: 11th Annual Meeting of international Society for Stem Cell Research (ISSCR)
  • Place of Presentation: Boston, USA
• [Presentation] iPS細胞を用いた今後の医療の可能性 (2013)
  • Author(s): 中畑龍俊
  • Organizer: 日本製薬医学会第4回年次大会
  • Place of Presentation: エーザイ株式会社本社5階ホール
  • Invited
• [Presentation] iPS細胞研究が切り開く未来の医療 (2013)
  • Author(s): 中畑龍俊
  • Organizer: 日本学術会議公開学術講演会「未来社会を築く生命科学と医療のフロンティア」
  • Place of Presentation: 京都大学薬学部記念講堂
  • Invited
• [Presentation] iPS細胞を用いた今後の小児科医療 (2013)
  • Author(s): 中畑龍俊
  • Organizer: 第84回日本小児科学会高知地方会
  • Place of Presentation: 高知医療センターくろしおホール
  • Invited
• [Presentation] さい帯血造血幹細胞発見秘話とiPS細胞ストックの臍帯血活用の未来像 (2013)
  • Author(s): 中畑龍俊
  • Organizer: さい帯血移植1万例突破記念事業「さらなる飛躍へのステップ」記念講演会
  • Place of Presentation: TKP田町カンファレンスセンター
  • Invited
• [Presentation] iPS細胞を活用した医療の可能性と倫理 (2013)
  • Author(s): 中畑龍俊
  • Organizer: 第10回STSフォーラム「科学技術が拓く人間の未来」公開シンポジウム
  • Place of Presentation: 京都商工会議所ビル講堂
  • Invited
• [Presentation] Xenograft model of TAM reveals the evolutionary process of myeloid leukemia in Down syndrome. (2013)
  • Author(s): Saida S., Watanabe K., Sato-Otsubo A., Terui K., Yoshida K., Okuno Y., Toki T., Wang R., Shiraishi Y., Miyano S., Kato I., Umeda K., Hiramatsu H., Fujino H., Adachi S., Nakahata T., Ito E., Ogawa S., Heike T.
  • Organizer: 第75回日本血液学会学術集会
  • Place of Presentation: 札幌市教育分化会館
• [Presentation] Clinical characteristics of 17 children with JMML who developed blast crisis. (2013)
  • Author(s): Honda Y., Tsuchida M., Masunaga A., Yoshimi A., Kojima S., Ito M., Kikuchi A., Nakahata T., Manabe A.
  • Organizer: 第75回日本血液学会学術集会
  • Place of Presentation: 札幌市教育分化会館
• [Presentation] iPS細胞の小児医療への応用 (2013)
  • Author(s): 中畑龍俊
  • Organizer: 第38回東日本小児科学会
  • Place of Presentation: 大宮ソニックシティ
  • Invited
• [Presentation] iPS細胞の臨床応用 (2013)
  • Author(s): 中畑龍俊
  • Organizer: 第55回日本小児血液・がん学会学術集会
  • Place of Presentation: ヒルトン福岡シーホーク
  • Invited
• [Presentation] Immunosuppressive therapy with antithymocyte globulin and Cyclosporine for fulminant aplastic anemia (2012)
  • Author(s): Yagasaki H., Watanabe K., Kudo K., Tsuchida M., Shichino H., Morimoto A., Kobayashi R., Yabe H., Kikuchi A., Ohga S., Ito E., Ohara A., Nakahata N., Kojima S.
  • Organizer: 54th Annual Meeting of the AMERICAN SOCIETY of HEMATOLOGY
  • Place of Presentation: Atlanta
• [Presentation] ips細胞研究の進展 (2012)
  • Author(s): 中畑龍俊
  • Organizer: 第59回日本臨床検査医学会学術集会
  • Place of Presentation: 国立京都国際会館
  • Invited
• [Presentation] 小児患者におけるips細胞の応用 (2012)
  • Author(s): 中畑龍俊
  • Organizer: 第49回日本小児アレルギー学会
  • Place of Presentation: 大阪国際会議場
• [Presentation] Differentiation of monocytic lineage cells from human iPS cells by using a serum and feeder free culture method (2012)
  • Author(s): Yanagimachi M., Niwa A., Tanaka T. Oshima K., Saito M., Nakahata T.
  • Organizer: 10◎<th>◎ Annual Meeting of International Society for Stem Cell Research (ISSCR)
  • Place of Presentation: Pacifico Yokohama, Japan
• [Presentation] Human ESC/IPSC-Derived mesenchymal stroma can support hematopoietic progenitors (2012)
  • Author(s): Niwa A., Saito M., Oshima K., Yanagimachi M., Tanaka T., Kato I., Nakahata T.
  • Organizer: 10◎<th>◎ Annual Meeting of International Society for Stem Cell Research (ISSCR)
  • Place of Presentation: Pacifico Yokohama, Japan
• [Presentation] Induced pluripotent stem cell model of severe congenital neutropenia with HAX1 gene deficiency (2012)
  • Author(s): Morishima T., Watanabe K., Niwa A., Tanaka T, Saida S, Kato I, Umeda K, Hiramatsu H., Matsubara K., Adachi S., Nakahata T., Heike T.
  • Organizer: 10th Annual Meeting of International Society for Stem Cell Research (ISSCR)
  • Place of Presentation: Pacifico Yokohama, Japan
• [Presentation] Induced pluripotent stem cells from CINCA syndrome patients as a model for dissecting somatic mosaicism and drug discovery (2012)
  • Author(s): Tanaka T., Saito M. K.., Takahashi K., Yamanaka S., Nakahata T.
  • Organizer: 10th Annual Meeting of International Society for Stem Cell Research (ISSCR)
  • Place of Presentation: Pacifico Yokohama, Japan
• [Presentation] 次世代シーケンサーによるNLRP3体制モザイクの診断 (2012)
  • Author(s): 井澤和司、土方敦司、西小森隆太、小原収、田中尚子、河合朋樹、八角高裕、斎藤潤、中畑龍俊、平家俊男
  • Organizer: 第115回日本小児科学会学術集会
  • Place of Presentation: 福岡国際会議場福岡市(口演)
• [Presentation] ips細胞研究の今、その可能性と将来展望 (2012)
  • Author(s): 中畑龍俊
  • Organizer: 第115回日本小児科学会学術集会
  • Place of Presentation: 福岡国際会議場
• [Presentation] iPS細胞研究の今、その可能性と将来展望 (2012)
  • Author(s): 中畑龍俊
  • Organizer: 第115回日本小児科学学術集
  • Place of Presentation: 福岡国際会議場
  • Invited
• [Presentation] 次世代シーケンサーによるNLRP3体制モザイクの診断 (2012)
  • Author(s): 井澤和司、土方敦司、西小森隆太、小原収、田中尚子、河合朋樹、八角高裕、斎藤潤、中畑龍俊、平家俊男
  • Organizer: 第115回日本小児科学学術集
  • Place of Presentation: 福岡国際会議場
• [Presentation] 白血球減少、骨髄異形成とリンパ浮腫を呈するGATA-2異常 (2012)
  • Author(s): 石田宏之、今井耕輔、本間健一、田村真一、今村俊彦、斎藤潤、大嶋宏一、伊藤雅文、中畑龍俊、野々山恵章
  • Organizer: 第115回日本小児科学学術集
  • Place of Presentation: 福岡国際会議場
• [Presentation] iPS細胞を用いたこれからの小児医療の可能性 (2012)
  • Author(s): 中畑龍俊
  • Organizer: 旭川小児科医会講演会
  • Place of Presentation: 旭川ロワジールホテル
  • Invited
• [Presentation] CINCA症候群におけるNLRP3体細胞モザイク変異 (2012)
  • Author(s): 井澤和司、西小森隆太、吉岡耕平、斉藤潤、中畑龍俊、平家俊男
  • Organizer: 第33回日本炎症・再生医学会
  • Place of Presentation: ホテル日航福岡
• [Presentation] iPS細胞から単球・樹状細胞の分化誘導と疾患iPS細胞研究への応用 (2012)
  • Author(s): 柳町昌克、丹羽明、田中孝之、村田祐樹、八角高裕、金澤伸雄、平家俊男、中畑龍俊、斎藤潤
  • Organizer: 第5回自己炎症疾患研究会
  • Place of Presentation: 福岡朝日ビル
• [Presentation] 小児患者におけるiPS細胞の応用 (2012)
  • Author(s): 中畑龍俊
  • Organizer: 第49回日本小児アレルギー学会
  • Place of Presentation: 大阪国際会議場
  • Invited
• [Presentation] iPS細胞研究の進展 (2012)
  • Author(s): 中畑龍俊
  • Organizer: 第59回日本臨床検査医学会学術集会
  • Place of Presentation: 国立京都国際会館
  • Invited
• [Presentation] Differentiation of monocytic lineage cells from human iPS cells by using a serum and feeder free culture method. (2012)
  • Author(s): Yanagimachi M, Niwa A, Tanaka T Oshima K, Saito M Nakahata T
  • Organizer: 10th Annual Meeting of International Society for Stem Cell Research (ISSCR)
  • Place of Presentation: Pacifico Yokohama
• [Presentation] Human ESC/IPSC-Derived mesenchymal stroma can support hematopoietic progenitors. (2012)
  • Author(s): Niwa A, Saito M, Oshima K, Yanagimachi M, Tanaka T, Kato I, Nakahata T
  • Organizer: 10th Annual Meeting of International Society for Stem Cell Research (ISSCR)
  • Place of Presentation: Pacifico Yokohama
• [Presentation] Induced pluripotent stem cell model of severe congenital neutropenia with HAX1 gene deficiency. (2012)
  • Author(s): Morishima T, Watanabe K, Niwa A, Tanaka T, Saida S, Kato I, Umeda K, Hiramatsu H, Matsubara K, Adachi S, Nakahata T, Heike T
  • Organizer: 10th Annual Meeting of International Society for Stem Cell Research (ISSCR)
  • Place of Presentation: Pacifico Yokohama
• [Presentation] Induced pluripotent stem cells from CINCA syndrome patients as a model for dissecting somatic mosaicism and drug discovery. (2012)
  • Author(s): Tanaka T, Saito MK., Takahashi K, Yamanaka S, Nakahata T
  • Organizer: 10th Annual Meeting of International Society for Stem Cell Research (ISSCR)
  • Place of Presentation: Pacifico Yokohama
• [Presentation] Clinic characteristics of 23 children with juvenile myelomonocytic leukemia who developed blast crisis: MDS Committee of LSPHO. (2012)
  • Author(s): Honda Y, Tsuchida M, Masunaga A, Yoshimi A, Kojima S, Ito M, Kikuchi A, Nakahata T, Manabe A
  • Organizer: VI Internatonal Symposium on Myelodysplastic Syndoromes and Bone Marrow Failures in Childhood
  • Place of Presentation: Diplomat Hotel, Prague
• [Presentation] Treatment outcome of 67 cases with refractory cytopenia of childhood(RCC): A Prostective Registration Through The Japanese Society of Pediatric Hematology/Oncology(JSPHO). (2012)
  • Author(s): Hasegawa D, Chen X-j, Hirabayashi S, Ishida Y, Watanabe S, Zaike Y, Tsuchida M, Masunaga A, Yoshimi A, Hama A, Kojima S, Ito M, Nakahata T, Manabe A
  • Organizer: VI Internatonal Symposium on Myelodysplastic Syndoromes and Bone Marrow Failures in Childhood
  • Place of Presentation: Diplomat Hotel, Prague
• [Presentation] NOGマウスを用いたTAMの病態解析 (2012)
  • Author(s): 才田聡、渡邉健一郎、宮野悟、加藤格、森嶋達也、梅田雄嗣、平松英文、藤野寿典、足立壮一、丹羽明、中畑龍俊、伊藤悦朗、小川誠司、平家俊男他７名１８番目
  • Organizer: 第54回日本小児血液・がん学会学術集会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Excess reduction of anthracyclines results in inferior event-free survival in core binding factor acute myeloid leukemia in children; A report from the Japanese Pediatric Leukemia/Lymphoma Study Group (JPLSG). (2012)
  • Author(s): Tomizawa D., Terui K., MD, Moritake H., Kinoshita A., Takahashi H., Nakayama H., Koh K., Kigasawa H., Kosaka Y., Miyachi H., Horibe K., Nakahata T., Adachi S.他７名１９番目
  • Organizer: 54th Annual Meeting of the AMERICAN SOCIETY of HEMATOLOGY
  • Place of Presentation: GeorGia World ConGress Center,Atlanta
• [Presentation] Mesodermal development from reprogrammed Fanconi anemia cells is affected by ALDH2 enzymatic activity. (2012)
  • Author(s): Suzuki N., Hira A., Niwa A., Saito M., Matsuo K., Nakahata T., Takata M., Yabe M.
  • Organizer: 54th Annual Meeting of the AMERICAN SOCIETY of HEMATOLOGY
  • Place of Presentation: GeorGia World ConGress Center,Atlanta
• [Presentation] Immunosuppressive therapy with antithymocyte globulin and Cyclosporine for fulminant aplastic anemia. (2012)
  • Author(s): Yagasaki H., Watanabe K., Kudo K., Tsuchida M., Shichino H., Morimoto A., Kobayashi R., Yabe H., Kikuchi A., Ohga S., Ito E., Ohara A., Nakahata N., Kojima S.
  • Organizer: 54th Annual Meeting of the AMERICAN SOCIETY of HEMATOLOGY
  • Place of Presentation: GeorGia World ConGress Center,
• [Remarks] 中畑研究室ホームページ:
  • URL: http://www.cira.kyoto-u.ac.jp/nakahata
• [Remarks] 中畑研究室ホームページ