| Project/Area Number |
24659498
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Pediatrics
|
|---|
| Research Institution | Shimane University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Keywords | 脂肪酸酸化 / セレウリド / タンデムマス法 / in vitro probe assay / 急性脳症 / 解熱剤 / heat stress / 脂肪酸β酸化 / β酸化能 / 低体温療法 / 細菌毒素 / 小児薬物 / 発熱ストレス |
|---|
| Outline of Final Research Achievements |
The mechanism of acute encephalopathy in young children is often unknown in many cases. Onset of acute encephalopathy is not infrequently similar to that of congenital fatty acid oxidation (FAO) disorders. Influence of bacterial toxins, antipyretic drugs, or heat stress by hyperpyrexia on FAO was determined by in vitro probe (IVP) assay using cultured fibroblasts, and tandem mass spectrometry, changing the circumstance of cell culture.
The results were as follows: (i) cereulide, a toxin of Bacillus cereus, which occasionally causes acute encephalopathy in infants, disturbed FAO; (ii) heat stress possibly causes exacerbation of FAO in long-chain FAO disorders; (iii) antipyretics, salicylate (metabolites of Aspirin), and Diclofenac disturbed FAO in normal control cells, while acetaminophen did not. These findings may be consistent with clinical observation. The above findings will be of help for prevention of acute encephalopathy in children with infectious disease and/or hyperpyrexia.
|
