| Project/Area Number |
24659507
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
TAKI Atsuko 東京医科歯科大学, 医学部附属病院, 助教 (20614481)
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| Co-Investigator(Renkei-kenkyūsha) |
KOMAKI Motohiro 東京医科歯科大学, 歯学部付属病院, 准教授 (30401368)
IWASAKI Kengo 東京医科歯科大学, 歯学部付属病院, 講師 (40401351)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 子宮内感染 / 脳室周囲白質軟化症 / 間葉系幹細胞 / 再生治療 / 子宮内感染合併症 / 新生児白質損傷モデル |
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| Outline of Final Research Achievements |
In this study we explore the therapeutic effects of MSC or MSC-conditioned medium (MSC-CM) on LPS-induced rat PVL model. Umbilical cord-derived MSC (UCMSC, Lonza) were purchased. Four-day old rats were intraperitoneally challenged with LPS to create PVL model and simultaneously treated by culture medium (control), MSC, or MSC-CM. Immuno-staining of myelin basic protein (MBP) in brain sections was used to evaluate cerebral white matter. Real time-PCR was performed to quantitate proinflammatory cytokine levels in 6-day-old rat brain.Postnatal LPS challenge caused PVL in rats, evaluated by increased inflammatory chytokines on day 6, and loss of MBP on day 12. Both MAS and MSC-CM significantly attenuated the cytokine level whereas only MSC attenuated loss of MBP.Our results showed that therapeutic potential of MSC due to anti-inflammatory effects in rat PVL model. The differences between MSC and MSC-CM suggests direct cellular effects are necessary for preventing MBP loss in PVL.
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