| Project/Area Number |
24659518
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dermatology
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| Research Institution | Hirosaki University |
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Principal Investigator |
SAWAMURA Daisuke 弘前大学, 医学(系)研究科(研究院), 教授 (60196334)
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| Co-Investigator(Kenkyū-buntansha) |
AKASAKA Eijiro 弘前大学, 医学部附属病院, 助教 (30436034)
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|---|
| Project Period (FY) |
2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | 皮膚 / 角化症 / 角質間脂質 / スピンラベル / ラジカル |
|---|
| Research Abstract |
Stratum corneum is the most outer layer of the skin and has barrier and defense function. Corneocytes and lipids between keratinocytes play an important role in the function. Spin labeling method is a sensitive and nondestructive technique to measure the probe mobility in the lipid bilayers. In this study, we introduced the probe into stratum corneum and evaluate function and structure of the lipids between corneal layers. We found that the pv-SC was less ordering of the structure than that of the control SC, indicating abnormal architecture of psoriasis vulgaris stratum corneum. This result is consistent with previous observations in the different methods. Therefore, we suggest that this EPR assay is of great use for evaluating SC function and can be extended to other skin diseases with abnormal keratinization.
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