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A rapid melanocyte-specific transgenesis system for phenotype-based gene function discovery

Research Project

Project/Area Number 24659524
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Dermatology
Research InstitutionGifu University

Principal Investigator

OSAWA Masatake  岐阜大学, 医学(系)研究科(研究院), 教授 (10344029)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords幹細胞 / 色素細胞 / トランスジェニックマウス / 遺伝子機能解析 / モデル動物 / ノックダウン / ES細胞 / 遺伝子改変マウス / 遺伝子ノックダウン
Research Abstract

The melanocyte affords an advantageous model for studying gene function through phenotype-based analysis, since alterations in genes involved in melanocyte regulation are easily identifiable as pigmentary phenotypes in the mouse. However, despite a number of recent methodological advances, the generation of genetically altered mice is still laborious and time-consuming, which largely hampers in vivo gene function assignment. Here, we have developed a novel melanocyte-specific transgenesis system, by which rapid gene functional assessment is allowed in mice. As a proof-of-concept study, we generated transgenic lines carrying shRNA targeting the Tyr, Mitf, Bcl2 or RBP-J genes and found that each transgenic mice faithfully recapitulated the pigmentary phenotype of the corresponding gene knockout mice. Overall, our study suggests the broad applicability of the transgenesis approach in understanding the molecular basis of melanocyte-related diverse biological phenomena.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (8 results)

All 2014 2013 2012

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (6 results) (of which Invited: 6 results) Book (1 results)

  • [Journal Article] BNIP3 plays crucial roles in the differentiation and maintenance of epidermal keratinocytes2013

    • Author(s)
      Moriyama M, Moriyama H, Uda J, Matsuyama A, Osawa M, Hayakawa T.
    • Journal Title

      J. Invest. Dermatol. jid

      Volume: (Epub ahead of print)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] A RAPID MELANOCYTE- SPECIFIC GENE DISRUPTION TO IDENTIFY GENES INVOLVED IN THE REGULATION OF MELANOCYTE STEM CELLS IN THE HAIR FOLLICLE J演者2014

    • Author(s)
      大沢匡毅
    • Organizer
      World Congress for Hair Research 2014
    • Place of Presentation
      韓国チェジュ島
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] A rapid melanocyte-specific gene disruption to identify genes involved in the regulation of melanocyte stem cells in the hair follicle.2014

    • Author(s)
      大沢 匡毅
    • Organizer
      8th World Congress for Hair Research
    • Place of Presentation
      韓国 デジュ島
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 簡便化ノックダウントランスジェニックマウスによる色素幹細胞制御遺伝子のスクリーニングJ演者2012

    • Author(s)
      大沢匡毅
    • Organizer
      第一回幹細胞を制御する微少環境の解明を目指した異分野交流研究会
    • Place of Presentation
      つくば市
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] メラノサイト機能遺伝子を同定するための簡便で迅速な遺伝子改変マウス作成法J演者2012

    • Author(s)
      大沢匡毅
    • Organizer
      第24回日本色素細胞学会学術大会
    • Place of Presentation
      長浜
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] ノッチシグナル系による皮膚上皮幹細胞系の制御J演者2012

    • Author(s)
      大沢匡毅
    • Organizer
      第2回細胞再生医療研究会
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] メラノサイト機能遺伝子を同定するための簡便で迅速な遺伝子改変マウス作成法2012

    • Author(s)
      大沢匡毅
    • Organizer
      第24回日本色素細胞学会学術大会
    • Place of Presentation
      長浜バイオ大学
    • Related Report
      2012 Research-status Report
    • Invited
  • [Book] 毛包形成のメカニズムの解明と毛包再生への応用2013

    • Author(s)
      大沢匡毅
    • Publisher
      シーエムシー出版
    • Related Report
      2013 Final Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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