Cholesterol ointment suppressed hapten-induced dermatitis by inducing 11B-HSD1

• Project/Area Number: 24659529
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Osaka University
• Principal Investigator: KATAYAMA ICHIRO 大阪大学, 医学(系)研究科(研究院), 教授 (80191980)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: アトピー性皮膚炎 / 皮膚バリア / コレステロール / 脂質 / コルチゾール / 11β-HSD1 / 表皮角化細胞 / 保湿剤 / 皮膚バリア機能 / 接触皮膚炎 / QOL、ステロイド外用薬 / バリア機能改善・抗炎症作用外用剤

Research Abstract

Changes in the stratum corneum extracellular matrix impair epidermal barrier function and may cause dermatoses in atopic dermatitis patients and elderly people. The aim of this study was to examine the effect of exogenous cholesterol application on skin barrier function and cutaneous inflammation. Hapten-stimulated mice were treated with topical cholesterol. The effect of topical cholesterol application on an oxazolone (OXA)-induced hypersensitivity reaction was evaluated. Topical application of cholesterol efficiently decreased transepidermal water loss in areas of barrier-disrupted skin and ameliorated OXA-induced cutaneous hypersensitivity. These favourable effects may have resulted from sustained expression of 11beta-hydroxysteroid dehydrogenase type 1 (11B-HSD1) in the cholesterol-treated skin. As 11B-HSD1 is known to produce active cortisol, topical cholesterol may attenuate contact hypersensitivity by normalizing secretion of hormonally active cortisol from the skin.

Research Products

• [Journal Article] 11β-hydroxysteroid dehydrogenase 1 specific inhibitor increased dermal collagen content and promotes fibroblast proliferatio (2014)
  • Author(s): Terao M, Tani M, Itoi S, Yoshimura T, Hamasaki T, Murota H, Katayama I.
  • Journal Title: PLoS One Volume: 9(3)
• [Journal Article] IFN-γor IFN-αAmeliorates Chronic Proliferative Dermatitis by Inducing Expression of Linear Ubiquitin Chain Assembly Complex (2014)
  • Author(s): Tamiya H, Terao M, Takiuchi T, Nakahara M, Sasaki Y, Katayama I, Yoshikawa H, Iwai K.
  • Journal Title: J Immunol Volume: 192(8) Pages: 3793-804
• [Journal Article] Topical cholesterol treatment ameliorates hapten-evoked cutaneous persensitivity by sustaining expression of 11β-HSD1 in epidermis (2014)
  • Author(s): Murota H, Itoi S, Terao M, Matsui S, Kawai H, Satou Y, Suda K, Katayama I.
  • Journal Title: Exp Dermatol Volume: 23(1) Pages: 68-70
• [Journal Article] 11β-hydroxysteroid dehydrogenase 1 specific inhibitor increased dermal collagen content and promotes fibroblast proliferation. (2014)
  • Author(s): Terao M, Tani M, Itoi S, Yoshimura T, Hamasaki T, Murota H, Katayama I.
  • Journal Title: PLoS One. Volume: Mar 25;9(3) Issue: 3 Pages: e93051-e93051
  • DOI: 10.1371/journal.pone.0093051
  • Peer Reviewed / Open Access
• [Journal Article] Expression profiles of cortisol-inactivating enzyme, 11β-hydroxysteroid dehydrogenase-2, in human epidermal tumors and its role in keratinocyte proliferation (2013)
  • Author(s): Terao M, Itoi S, Murota H, Katayama I.
  • Journal Title: Exp Dermatol Volume: 22(2) Pages: 98-101
• [Journal Article] 11β-Hydroxysteroid dehydrogenase 1 contributes to the pro-inflammatory response of keratinocytes (2013)
  • Author(s): Itoi S, Terao M, Murota H, Katayama I.
  • Journal Title: Biochem Biophys Res Commun Volume: 440(2) Pages: 265-70
• [Journal Article] Barrier abnormality due to ceramide deficiency leads to psoriasiform inflammation in a mouse model (2013)
  • Author(s): Nakajima K, Terao M, Takaishi M, Kataoka S, Goto-Inoue N, Setou M, Horie K, Sakamoto F, Ito M, Azukizawa H, Kitaba S, Murota H, Itami S, Katayama I, Takeda J, Sano S.
  • Journal Title: J Invest Dermatol Volume: 133(11) Pages: 2555-65
• [Journal Article] Expression profiles of cortisol-inactivating enzyme, 11β-hydroxysteroid dehydrogenase-2, in human epidermal tumors and its role in keratinocyte proliferation. (2013)
  • Author(s): Terao M, Itoi S, Murota H, Katayama I.
  • Journal Title: Exp Dermatol. Volume: Feb;22(2) Issue: 2 Pages: 98-101
  • DOI: 10.1111/exd.12075
  • Peer Reviewed
• [Journal Article] 11β-Hydroxysteroid dehydrogenase 1 contributes to the pro-inflammatory response of keratinocytes. (2013)
  • Author(s): Itoi S, Terao M, Murota H, Katayama I.
  • Journal Title: Biochem Biophys Res Commun. Volume: Oct 18;440(2) Issue: 2 Pages: 265-70
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  • Peer Reviewed
• [Journal Article] Prevalence and Impact of Past History of Food Allergy in Atopic Dermatitis (2013)
  • Author(s): Kijima A, Murota H, Takahashi A, Arase N, Yang L, Nishioka M, Yamaoka T, Kitaba S, Yamauchi-Takihara K, Katayama I.
  • Journal Title: Allergology International Volume: 62 Issue: 1 Pages: 105-112
  • DOI: 10.2332/allergolint.12-OA-0468
  • NAID: 130004477175
  • ISSN: 1323-8930, 1440-1592
  • Peer Reviewed
• [Journal Article] Increased frequency of skin-infiltrating FoxP3+ regulatory T cells as a diagnostic indicator of severe atopic dermatitis from cutaneous T cell lymphoma. (2013)
  • Author(s): Hanafusa T, Matsui S, Murota H, Tani M, Igawa K, Katayama I.
  • Journal Title: Clin Exp Immunol. Volume: Jun;172(3) Issue: 3 Pages: 507-12
  • DOI: 10.1111/cei.12073
  • Peer Reviewed
• [Journal Article] Barrier abnormality due to ceramide deficiency leads to psoriasiform inflammation in a mouse model. (2013)
  • Author(s): Nakajima K, Terao M, Takaishi M, Kataoka S, Goto-Inoue N, Setou M, Horie K, Sakamoto F, Ito M, Azukizawa H, Kitaba S, Murota H, Itami S, Katayama I, Takeda J, Sano S.
  • Journal Title: J Invest Dermatol. Volume: 133 Issue: 11 Pages: 2555-2565
  • DOI: 10.1038/jid.2013.199
  • Peer Reviewed
• [Journal Article] Trends in the prevalence of tuberous sclerosis complex manifestations: an epidemiological study of 166 Japanese patients. (2013)
  • Author(s): Wataya-Kaneda M, Tanaka M, Hamasaki T, Katayama I.
  • Journal Title: PLoS One Volume: May 17;8(5) Issue: 5 Pages: e63910-e63910
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• [Journal Article] Development and clinical study of a self-dissolving microneedle patch for transcutaneous immunization device (2013)
  • Author(s): Sachiko Hirobe, Hiroaki Azukizawa, Kazuhiko Matsuo, You Zhai, Ying-Shu Quan, Fumio Kamiyama, Hiroshi Suzuki, Ichiro Katayama, Naoki Okada, and Shinsaku Nakagawa
  • Journal Title: Pharm. Res. Volume: 30(10) Issue: 10 Pages: 2664-2674
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• [Journal Article] CD1a-positive familial cutaneous mastocytosis without germ-line or somatic mutations in c-kit (2013)
  • Author(s): Arase, N., Wataya-Kaneda, M., Oiso, N., Arase, H., Katayama, I.
  • Journal Title: Br. J. Dermatol. Volume: 169 Issue: 1 Pages: 201-204
  • DOI: 10.1111/bjd.12265
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• [Journal Article] Alopecia areata: infiltration of Th17 cells in the dermis, particularly around hair follicles. (2013)
  • Author(s): Tanemura A, Oiso N, Nakano M, Itoi S, Kawada A, Katayama I.
  • Journal Title: Dermatology. Volume: 226(4) Issue: 4 Pages: 333-6
  • DOI: 10.1159/000350933
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• [Journal Article] First left-right comparative study of topical rapamycin vs. vehicle for facial angiofibromas in patients with tuberous sclerosis complex. (2013)
  • Author(s): Tanaka M, Wataya-Kaneda M, Nakamura A, Matsumoto S, Katayama I.
  • Journal Title: Br J Dermatol. Volume: Dec;169(6) Issue: 6 Pages: 1314-8
  • DOI: 10.1111/bjd.12567
  • Peer Reviewed
• [Journal Article] Decreased Sudomotor Function is Involved in the Formation of Atopic Eczema in the Cubital Fossa (2013)
  • Author(s): Takahashi A, Murota H, Matsui S, Kijima A, Kitaba S, Lee JB, Katayama I.
  • Journal Title: Allergology International Volume: 62 Issue: 4 Pages: 473-478
  • DOI: 10.2332/allergolint.13-OA-0547
  • NAID: 130004873087
  • ISSN: 1323-8930, 1440-1592
  • Peer Reviewed
• [Journal Article] Karyopherin alpha2 is essential for rRNA transcription and protein synthesis in proliferative keratinocytes (2013)
  • Author(s): Umegaki-Arao N, Tamai K, Nimura K, Serada S, Naka T, Nakano H, Katayama I
  • Journal Title: PLoS One Volume: 8 Issue: 10 Pages: e76416-e76416
  • DOI: 10.1371/journal.pone.0076416
  • Peer Reviewed
• [Journal Article] Recent advances and developments in the antitumor effect of the HVJ envelope vector on malignant melanoma: from the bench to clinical application. (2013)
  • Author(s): Tanemura A, Kiyohara E, Katayama I, Kaneda Y.
  • Journal Title: Cancer Gene Ther. Volume: Nov;20(11) Issue: 11 Pages: 599-605
  • DOI: 10.1038/cgt.2013.61
  • Peer Reviewed
• [Journal Article] Periostin Facilitates Skin Sclerosis via PI3K/Akt Dependent Mechanism in a Mouse Model of Scleroderma (2012)
  • Author(s): Yang L, Serada S, Fujimoto M, Terao M, Kotobuki Y, Kitaba S, Matsui S, Kudo A, Naka T, Murota H, Katayama I
  • Journal Title: PLoS One Volume: 7(7) Issue: 7 Pages: e41994-e41994
  • DOI: 10.1371/journal.pone.0041994
  • Peer Reviewed
• [Journal Article] Positive link between STAT3 activation and Th17 cell infiltration to the lesional skin in vitiligo vulgaris. (2012)
  • Author(s): Tanemura A, Kotobuki Y, Itoi S, Takata T, Sano S, Katayama I.
  • Journal Title: J Dermatol Sci. Volume: 67(3) Issue: 3 Pages: 207-9
  • DOI: 10.1016/j.jdermsci.2012.06.010
  • Peer Reviewed
• [Journal Article] Artemin causes hypersensitivity to warm sensation, mimicking warmth-provoked pruritus in atopic dermatitis. (2012)
  • Author(s): Murota H, Izumi M, Abd El-Latif MI, Nishioka M, Terao M, Tani M, Matsui S, Sano S, Katayama I.
  • Journal Title: Journal of Allergy and Clinical Immunology Volume: 130 Issue: 3 Pages: 671-682
  • DOI: 10.1016/j.jaci.2012.05.027
  • Peer Reviewed
• [Journal Article] Upregulation of N-acetylglucosaminyltransferase-V by heparin-binding EGF-like growth factor induces keratinocyte proliferation and epidermal hyperplasia. (2012)
  • Author(s): Kimura A, Terao M, Kato A, Hanafusa T, Murota H, Katayama I, Miyoshi E.
  • Journal Title: Exp Dermatol. Volume: 21(7) Issue: 7 Pages: 515-9
  • DOI: 10.1111/j.1600-0625.2012.01515.x
  • Peer Reviewed
• [Journal Article] Periostin, a matricellular protein, accelerates cutaneous wound repair by activating dermal fibroblasts (2012)
  • Author(s): Ontsuka K, Kotobuki Y, Shiraishi H, Serada S, Ohta S, Tanemura A, Yang L, Fujimoto M, Arima K, Suzuki S, Murota H, Toda S, Kudo A, Conway S, Izuhara K, Katayama I, Naka T
  • Journal Title: Experimental Dermatology Volume: 21(5) Issue: 5 Pages: 331-336
  • DOI: 10.1111/j.1600-0625.2012.01454.x
  • Peer Reviewed
• [Journal Article] Dysregulation of melanocyte function by Th17-related cytokines : significance of Th17 cell infiltration in autoimmune vitiligo vulgaris (2012)
  • Author(s): Kotobuki Y, Tanemura A, Yang L, Itoi S, Wataya-Kaneda M, Murota H, Fujimoto M, Serada S, Naka T, Katayama I
  • Journal Title: Pigment Cell Melanoma Res Volume: 25(2) Issue: 2 Pages: 219-230
  • DOI: 10.1111/j.1755-148x.2011.00945.x
  • Peer Reviewed
• [Journal Article] A novel application of topical rapamycin formulation, an inhibitor of mTOR, for patients with hypomelanotic macules in tuberous sclerosis complex. (2012)
  • Author(s): Wataya-Kaneda M, Tanaka M, Nakamura A, Matsumoto S, Katayama I.
  • Journal Title: Arch Dermatol Volume: 148(1) Issue: 1 Pages: 138-9
  • DOI: 10.1001/archderm.148.1.138
  • Peer Reviewed
• [Journal Article] Sev ere dermatitis with loss of epidermal Langerh ans cells in human and mouse zinc deficiency. (2011)
  • Author(s): Kawamura T, Ohta Y, et al.
  • Journal Title: The Journal of Clinical Investigation Volume: 84 Issue: 2 Pages: 722-732
  • DOI: 10.1172/jci58618
  • Peer Reviewed
• [Presentation] Topical Vitamin D3 Therapy for Steroid Resistant Prurigo : Anti-inflammatory Action or Neuroprotective Effect (2013)
  • Author(s): Ichiro Katayama, Mika Terao, Mayuko Nakano, SJ Bae, Hiroyuki Murota
  • Organizer: 7th World Congress on ITCH
  • Place of Presentation: Boston, USA
  • Year and Date: 2013-09-21
• [Presentation] Upregulation of periostin by histamine via ERK1/2 pathway is essential for histamine-induced collagen production in dermal fibroblasts (2013)
  • Author(s): Linli Yang, Hiroyuki Murota, Saki Matsui, Mika Terao, Ichiro Katayama
  • Organizer: 2013 International Investigative Dermatology Meeting
  • Place of Presentation: Edinburgh, Scotland
  • Year and Date: 2013-05-09
• [Presentation] Oligosaccharide modification by N-acetylglucosaminyltransferase-V promotes skin sclerosis by inducing macrophages to shift toward M2 (2013)
  • Author(s): Mika Terao, Arisa Kato, Mizuki Yutani, Hiroyuki Murota, Eiji Miyoshi, Ichiro Katayama
  • Organizer: 2013 International Investigative Dermatology Meeting
  • Place of Presentation: Edinburgh, Scotland
  • Year and Date: 2013-05-09
• [Presentation] Topical cholesterol application ameliorates hapten-evoked cutaneous hypersensitivity by sustaining expression of 11βHSD-1 in epidermis (2013)
  • Author(s): H Murota, M Terao, S Matsui, H Kawai, Y Satou, K Suda, I Katayama
  • Organizer: 2013 International Investigative Dermatology Meeting
  • Place of Presentation: Edinburgh, Scotland
  • Year and Date: 2013-05-08
• [Presentation] Expression profiles of cortisol inactivating enzyme, 11β-hydroxysteroid dehydrogenase 1 and 2 in human epidermal tumors and its role in keratinocyte proliferation (2013)
  • Author(s): Mika Terao, Hiroyuki Murota, Ichiro Katayama
  • Organizer: 1st Dermatoendocrinology Meeting
  • Place of Presentation: Edinburgh, Scotland
  • Year and Date: 2013-05-07
• [Presentation] Oligosaccharide modification by N-acetylglucosaminyltransferase-V promotes skin sclerosis by inducing macrophages to shift toward M2. (2013)
  • Author(s): Mika Terao, Arisa Kato, Mizuki Yutani, Hiroyuki Murota, Eiji Miyoshi, Ichiro Katayama
  • Organizer: 2013 International Investigative Dermatology Meeting
  • Place of Presentation: Edinburgh, Scotland
• [Presentation] Expression profiles of cortisol inactivating enzyme, 11β-hydroxysteroid dehydrogenase 1 and 2 in human epidermal tumors and its role in keratinocyte proliferation. (2013)
  • Author(s): Mika Terao, Hiroyuki Murota, Ichiro Katayama
  • Organizer: 1st Dermatoendocrinology Meeting
  • Place of Presentation: Edinburgh, Scotland
• [Presentation] Topical cholesterol application ameliorates hapten-evoked cutaneous hypersensitivity by sustaining expression of 11βHSD-1 in epidermis. (2013)
  • Author(s): H Murota, M Terao, S Matsui, H Kawai, Y Satou, K Suda, I Katayama
  • Organizer: 2013 International Investigative Dermatology Meeting
  • Place of Presentation: Edinburgh, Scotland
• [Presentation] Topical Vitamin D3 Therapy for Steroid Resistant Prurigo: Anti-inflammatory Action or Neuroprotective Effect (2013)
  • Author(s): Ichiro Katayama, Mika Terao, Mayuko Nakano, SJ Bae, Hiroyuki Murota
  • Organizer: 7th World Congress on ITCH
  • Place of Presentation: Boston, USA
• [Remarks]
  • URL: http://derma.med.osaka-u.ac.jp/