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Development of activator for skin function using bone marrow mesenchymal stem cell mobilizer

Research Project

Project/Area Number 24659530
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Dermatology
Research InstitutionOsaka University

Principal Investigator

TAMAI Katsuto  大阪大学, 医学系研究科, 寄附講座教授 (20236730)

Project Period (FY) 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
KeywordsHMGB1 / 間葉系幹細胞 / 表皮水疱症 / 骨髄間葉系幹細胞 / 再生医学
Research Abstract

Using skin transplantation system on GFP-transgenic bone marrow-transplanted mouse, bone marrow-derived mesenchymal stem cells (MSCs) in the skin graft were evaluated by flow cytometry analysis. As a result, bone marrow -derived MSCs in the skin graft increased time dependently, and reached to ~1 % of all cell population in the skin graft at day 7 after the engraftment. Further precise investigation revealed that particular cell population (PDGFRα+/SSEA3+) in MSCs is a pluripotent stem cells harboringkeratinocyte-differentiation potential. Furthermore, intravenous administration of HMGB1, which had been shown to mobilize bone marrow MSCs into the circulation, showed potent activities to improve function of the skin graft by increasing MSC numbers, enhancing angiogenesis, and suppressing inflammation in the skin graft.

Report

(2 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • Research Products

    (23 results)

All 2013 2012 2011

All Journal Article (9 results) (of which Peer Reviewed: 3 results) Presentation (12 results) (of which Invited: 3 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 2 results)

  • [Journal Article] Systemic Circulation and Bone Recruitment of Osteoclast Precursors Tracked by Using Fluorescent Imaging Techniques.2013

    • Author(s)
      Kotani M, Kikuta J, Klauschen F, Chino T, Kobayashi Y, Yasuda H, Tamai K, Miyawaki A, Kanagawa O, Tomura M, Ishii M
    • Journal Title

      J Immunol

      Volume: 190(2) Pages: 605-612

    • Related Report
      2012 Final Research Report
  • [Journal Article] Systemic administration of a novel immune-stimulatory pseudovirion suppresses lung metastatic melanoma by regionally enhancing IFN-γ production2013

    • Author(s)
      Saga K, Tamai K, Yamazaki T, Kaneda Y
    • Journal Title

      Clin Cancer Res

      Volume: 19(3) Pages: 668-679

    • Related Report
      2012 Final Research Report
  • [Journal Article] Early intra-amniotic gene transfer using lentiviral vector improves skin blistering phenotype in a murine model of Herlitz junctional epidermolysis bullosa.2012

    • Author(s)
      Endo M, Zoltick PW, Radu A, Qiujie J, Matsui C, Marinkovich PM, McGrath J, Tamai K, Uitto J, Flake AW
    • Journal Title

      Gene Ther

      Volume: 19(5) Issue: 5 Pages: 561-9

    • DOI

      10.1038/gt.2011.135

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Journal Article] HIG1, a novel regulator of mitochondrial γ-secretase, maintains normal mitochondrial function2012

    • Author(s)
      Hayashi H, Nakagami H, Takeichi M, Shimamura M, Koibuchi N, Oiki E, Sato N, Koriyama H, Mori M, Gerardo Araujo R, Maeda A, Morishita R, Tamai K, Kaneda Y
    • Journal Title

      FASEB J

      Volume: 26(6) Pages: 2306-2317

    • Related Report
      2012 Final Research Report
  • [Journal Article] Two cases of recessive dystrophic epidermolysis bullosa diagnosed as severe generalized2012

    • Author(s)
      Ohashi M, Shu E, Nagai M, Murase K, Nakano H, Tamai K, Sawamura D, Hiroka T, Seishima M, Kitajima Y, Aoyama Y
    • Journal Title

      J Dermatol

      Volume: 38 Pages: 893-9

    • NAID

      10030728065

    • Related Report
      2012 Final Research Report
  • [Journal Article] The course of pregnancy and childbirth in three mothers with recessive dystrophic epidermolysis bullosa2012

    • Author(s)
      Hanafusa T, Tamai K, Umegaki N, Yamaguchi Y, Fukuda S, Nishikawa Y, Yaegashi N, Okuyama R, McGrath JA, Katayama I
    • Journal Title

      Clin Exp Dermatol

      Volume: 37 Pages: 10-4

    • Related Report
      2012 Final Research Report
  • [Journal Article] Systemic administration of a novel immune-stimulatory pseudovirion suppresses lung metastatic melanoma by regionally enhancing IFN-γ production2012

    • Author(s)
      Saga K, Tamai K, Yamazaki T, Kaneda Y
    • Journal Title

      Clin Cancer Res

      Volume: Epub ahead of print

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Systemic Circulation and Bone Recruitment of Osteoclast Precursors Tracked by Using Fluorescent Imaging Techniques2012

    • Author(s)
      Kotani M, Kikuta J, Klauschen F, Chino T, Kobayashi Y, Yasuda H, Tamai K, Miyawaki A, Kanagawa O, Tomura M, Ishii M
    • Journal Title

      J Immunol

      Volume: Epub ahead of print

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Two cases of recessive dystrophic epidermolysis bullosa diagnosed as severe generalized2012

    • Author(s)
      5. Ohashi M, Shu E, Nagai M, Murase K, Nakano H, Tamai K, Sawamura D, Hiroka T, Seishima M, Kitajima Y, Aoyama Y
    • Journal Title

      J Dermatol

      Volume: 38 Pages: 893-899

    • NAID

      10030728065

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] 他家骨髄間葉系幹細胞移植による表皮水疱症治療研究:基礎と臨床2013

    • Author(s)
      玉井克人
    • Organizer
      第12回日本再生医療学会総会
    • Place of Presentation
      横浜
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 幹細胞研究の展望:末梢循環性間葉系幹細胞の皮膚集積による病態変化2012

    • Author(s)
      玉井克人
    • Organizer
      日本皮膚科学会中部支部学術大会
    • Place of Presentation
      大阪
    • Year and Date
      2012-10-13
    • Related Report
      2012 Final Research Report
  • [Presentation] 水疱症に対する骨髄間葉系幹細胞移植治療の可能性2012

    • Author(s)
      玉井克人、飯沼晋、菊池康、飯塚一、金田安史、片山一朗
    • Organizer
      第34回水疱症研究会
    • Place of Presentation
      弘前
    • Year and Date
      2012-10-06
    • Related Report
      2012 Final Research Report
  • [Presentation] Injured tissue releases HMGB1 to recruit bone marrow mesenchymal stem cells for inducing regeneration of the injury2012

    • Author(s)
      Katsuto Tamai, Yasushi Kikuchi, Shin Iinuma, Yasufumi Kaneda
    • Organizer
      The 71st Annual Meeting of the Japanese Cancer Association
    • Place of Presentation
      Sapporo, Japan
    • Year and Date
      2012-09-20
    • Related Report
      2012 Final Research Report
  • [Presentation] アトピー性皮膚炎に対するNFkB デコイ DNA 軟膏の展望2012

    • Author(s)
      玉井克人
    • Organizer
      第42回日本皮膚アレルギー・接触皮膚炎学会総会学術大会
    • Place of Presentation
      軽井沢
    • Year and Date
      2012-07-14
    • Related Report
      2012 Final Research Report
  • [Presentation] 教育講演 49 遙かにひろがる皮膚イメージング宇宙の旅:皮膚イメージング宇宙を旅する宇宙船皮膚号にようこそ.2012

    • Author(s)
      玉井克人
    • Organizer
      第111回日本皮膚科学会総会・学術大会
    • Place of Presentation
      京都
    • Year and Date
      2012-06-03
    • Related Report
      2012 Final Research Report
  • [Presentation] 教育講演 6 研究を目指す若手皮膚科医のために 臨床医にとって研究とは? 臨床と研究は皮膚科学一卵性双生児である2012

    • Author(s)
      玉井克人
    • Organizer
      第111回日本皮膚科学会総会・学術大会
    • Place of Presentation
      京都
    • Year and Date
      2012-06-01
    • Related Report
      2012 Final Research Report
  • [Presentation] Crosstalk mechanism between injured epithelia and bone marrow2012

    • Author(s)
      Katsuto Tamai
    • Organizer
      Experimental biology 2012
    • Place of Presentation
      SanDiego
    • Related Report
      2012 Annual Research Report
  • [Presentation] Mesenchymal stem cell transplantation for epidermolysis bullosa2012

    • Author(s)
      Katsuto Tamai
    • Organizer
      75th Annual Meeting of Society of Investigatilve Dermatology
    • Place of Presentation
      Raleigh, North Carolina
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Injured tissue releases HMGB1 to recruit bone marrow mesenchymal stem cells for inducing regeneration of the injury2012

    • Author(s)
      Katsuto Tamai
    • Organizer
      The 71st Annual Meeting of the Japanese Cancer Association
    • Place of Presentation
      Sapporo
    • Related Report
      2012 Annual Research Report
  • [Presentation] 末梢循環性間葉系幹細胞の皮膚集積による病態変化2012

    • Author(s)
      玉井克人
    • Organizer
      第63回日本皮膚科学会中部支部学術集会
    • Place of Presentation
      大阪
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 損傷組織と骨髄細胞のクロストークを利用した体内再生誘導医療開発2011

    • Author(s)
      玉井克人、飯沼晋、菊池康、金田安史
    • Organizer
      第11回日本再生医療学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2011-06-12
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 組織再生を誘導するためのペプチドとその利用2012

    • Inventor(s)
      玉井克人他
    • Industrial Property Rights Holder
      大阪大学、株式会社ジェノミックス
    • Filing Date
      2012-04-03
    • Related Report
      2012 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 組織再生を誘導するためのペプチドとその利用2012

    • Inventor(s)
      玉井克人
    • Industrial Property Rights Holder
      玉井克人
    • Industrial Property Rights Type
      特許
    • Filing Date
      2012-04-03
    • Related Report
      2012 Annual Research Report
    • Overseas

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Published: 2013-05-31   Modified: 2019-07-29  

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