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Development of a functional diagnostic system for angiosarcoma by surface plasmon resonance

Research Project

Project/Area Number 24659531
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Dermatology
Research InstitutionHiroshima University

Principal Investigator

HIDE Michihiro  広島大学, 医歯薬保健学研究院, 教授 (50284188)

Co-Investigator(Renkei-kenkyūsha) YANASE Yuhki  広島大学, 医歯薬保健学研究院, 助教 (40452586)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords血管肉腫 / 皮膚悪性腫瘍 / バイオセンサー / 表面プラズモン共鳴 / 血管内皮細胞 / 細胞内情報伝達
Research Abstract

We applied surface plasmon resonance (SPR) for the analysis of circulating tumor cells (CTC) derived from angiosarcoma. We could recover up to 10 angiosarcoma cells from a mixture of 1 ml of human peripheral blood, mixed with 2,000 angiosarcoma cells and anti-CD146 antibodies. Both angiosarcoma cells and normal vascular endothelial cells expressed VEGF receptors on their surface, and one of two angiosarcoma cell lines phosphorylated their EVGF receptors in response to VEGF. However, only normal endothelial cells showed an SPR signal by VEGF. These results suggest that SPR biosensor may detect CTC of angiosarcoma both sensitively and rapidly.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (52 results)

All 2014 2013 2012

All Journal Article (25 results) (of which Peer Reviewed: 14 results,  Open Access: 1 results) Presentation (25 results) (of which Invited: 2 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] A New Reliable Method for Detecting Specific IgE Antibodies in the Patients with Immediate Type Wheat Allergy due to Hydrolyzed Wheat Protein: Correlation of Its Titer and Clinical Severity2014

    • Author(s)
      Nakamura M., Yagami A.,Hara K., Sano A., Kobayashi T., Aihara M., Hide M.,Chinuki Y., Morita E., Teshima R., Matsunaga K
    • Journal Title

      Allergology International

      Volume: 63 Issue: 2 Pages: 243-249

    • DOI

      10.2332/allergolint.13-OA-0618

    • NAID

      130004477211

    • ISSN
      1323-8930, 1440-1592
    • Related Report
      2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] SPRイメージングによるアレルギー診断法2014

    • Author(s)
      小原隆之,柳瀬雄輝,隈崎修孝,秀道広
    • Journal Title

      Bio Clinic

      Volume: 29 Pages: 68-73

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Non-tumor mast cells cultured in vitro on a honeycomb-like structured film proliferate but downregulate with multinucleated formation2014

    • Author(s)
      Hyunmi Choi, Masaru Tanaka, Takaaki Hiragun, Michihiro Hide, Koreaki Sugimoto
    • Journal Title

      Nanomedicine

      Volume: Vol.10 Issue: 2 Pages: 213-219

    • DOI

      10.1016/j.nano.2013.08.011

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A new reliable method for detecting specific IgE antibodies in the patients with immediate type wheat allergy due to hydrolyzed wheat protein: Correlation of its titer and clinical severity2014

    • Author(s)
      Nakamura M, Yagami A, Hara K, Sano A, Kobayashi T, Aihara M, Hide M, Chinuki Y, Morita E, Teshima R, Matsunaga K.
    • Journal Title

      Allergol Int

      Volume: in press

    • NAID

      130004477211

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] SPRイメージングによるアレルギー診断法2014

    • Author(s)
      小原 隆之、柳瀬 雄輝、隈崎 修孝 、秀 道広
    • Journal Title

      Bio Clinica

      Volume: 29 Pages: 68-73

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Diagnostic tests for urticaria2013

    • Author(s)
      Hide M, Hiragun M, Hiragun T
    • Journal Title

      Immunology and Allergy Clinics of North America

      Volume: 34 Issue: 1 Pages: 53-72

    • DOI

      10.1016/j.iac.2013.09.009

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Aspirin Augments IgE-Mediated Histamine Release from Human Peripheral Basophils via Syk Kinase Activation2013

    • Author(s)
      Matsuo H, Yokooji T, Morita H, Ooi M, Urata K, Ishii K, Takahagi S, Yanase Y, Hiragun T, Mihara S, Hide M
    • Journal Title

      Allergology International

      Volume: 62 Issue: 4 Pages: 503-511

    • DOI

      10.2332/allergolint.13-OA-0536

    • NAID

      130004873086

    • ISSN
      1323-8930, 1440-1592
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Sensitivity and Clinical Course of Patients with Wheat-Dependent Exercise-Induced Anaphylaxis Sensitized to Hydrolyzed Wheat Protein in Facial Soap - Secondary Publication2013

    • Author(s)
      Hiragun M, Ishii K, Hiragun T, Shindo H, Mihara S, Matsuo H, Hide M.
    • Journal Title

      Allergology International

      Volume: 62 Issue: 3 Pages: 351-358

    • DOI

      10.2332/allergolint.13-OA-0553

    • NAID

      130004477197

    • ISSN
      1323-8930, 1440-1592
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] An autopsy case of metastatic extramammary Paget's disease treated with multimodality treatment including anti-HER2 therapy : What is the clinical and pathological significance of trastuzumab to the patient?2013

    • Author(s)
      Yoshimura N, Arihiro K, Takahagi S, Hide M
    • Journal Title

      Modern Chemotherapy

      Volume: 2 Issue: 04 Pages: 66-68

    • DOI

      10.4236/mc.2013.24008

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Application of SPR Imaging Sensor for Detection of Individual Living Cell Reactions and Clinical Diagnosis of Type I Allergy2013

    • Author(s)
      Yanase Y, Hiragun T, Yanase T, Kawaguchi T, Ishii K, Hide M
    • Journal Title

      Allergology International

      Volume: 62 Issue: 2 Pages: 163-169

    • DOI

      10.2332/allergolint.12-RA-0505

    • NAID

      130004873076

    • ISSN
      1323-8930, 1440-1592
    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 広島大学皮膚科における血管肉腫の治療経験2013

    • Author(s)
      菅崇暢,栁瀬哲至,河合幹雄,秀道広,間所直樹,野田英貴,岩崎泰政
    • Journal Title

      西日本皮膚科

      Volume: 75 Pages: 100-101

    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Journal Article] 好酸球性蜂窩織炎を合併した皮膚悪性リンパ腫の1例2013

    • Author(s)
      堀郁子,坂本旭,森本謙一,米原修治,松阪由紀,河合幹雄,秀道広,壷井ひとみ
    • Journal Title

      西日本皮膚科

      Volume: 75 Pages: 100-100

    • Related Report
      2013 Final Research Report
  • [Journal Article] An autopsy case of metastatic extramammary Paget's disease treated with multimodality treatment including anti-HER2 therapy: What is the clinical and pathological significance of trastumab to the patient?2013

    • Author(s)
      Yoshimura N, Arihiro K, Takahagi S, Hide M
    • Journal Title

      Modern Chemotherapy

      Volume: 2 Pages: 66-68

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Application of SPR Imaging Sensor for Detection of Individual Living Cell Reactions and Clinical Diagnosis of Type I Allergy.2013

    • Author(s)
      Yanase Y, Hiragun T, Yanase T, Kawaguchi T, Ishii K, Hide M.
    • Journal Title

      Allergol Int

      Volume: in press

    • NAID

      130004873076

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 好酸球性蜂窩織炎を合併した皮膚悪性リンパ腫の1例2013

    • Author(s)
      堀 郁子, 坂本 旭, 森本 謙一, 米原 修治, 松阪 由紀, 河合 幹雄, 秀 道広, 壷井 ひとみ
    • Journal Title

      西日本皮膚科

      Volume: 75 Pages: 100-100

    • Related Report
      2012 Research-status Report
  • [Journal Article] Elevated interleukin-18 secretion from monoclonal IgM+ B cells in a patient with Schnitzler syndrome2012

    • Author(s)
      Bhattacharyya J, Mihara K, Morimoto K, Takihara Y, Hide M
    • Journal Title

      J Am Acad Dermatol

      Volume: 67 Issue: 3 Pages: 118-20

    • DOI

      10.1016/j.jaad.2011.10.002

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cutaneous myoepithelial carcinomaの1例2012

    • Author(s)
      菅崇暢,中村千春,柳瀬哲至,河合幹雄,秀道広
    • Journal Title

      日本形成外科学会会誌

      Volume: 32 Pages: 504-505

    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Journal Article] 顆粒球コロニー刺激因子産生有棘細胞癌の1例2012

    • Author(s)
      高坂卓馬,原武,間所直樹,秀道広,大原直樹
    • Journal Title

      皮膚科の臨床

      Volume: 54 Pages: 1017-1020

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 下腹部に生じた皮下腫瘤2012

    • Author(s)
      高坂卓馬,信藤肇,秀道広,原武,行徳英一,西阪隆
    • Journal Title

      日本皮膚病理組織学会会誌

      Volume: 27 Pages: 63-63

    • Related Report
      2013 Final Research Report
  • [Journal Article] プロプラノロールが有効であった巨大な乳児血管腫の1例2012

    • Author(s)
      戸田さゆり,田中麻衣子,河合幹雄,秀道広,唐川修平,青木恵美,宮本義洋
    • Journal Title

      西日本皮膚科

      Volume: 74 Pages: 394-398

    • NAID

      130004475469

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] プロプラノロール内服療法が奏効した顔面の巨大なイチゴ状血管腫の1例2012

    • Author(s)
      田中麻衣子,栁瀬哲至,河合幹雄,信藤肇,秀道広,青木恵美,宮本義洋,唐川修平
    • Journal Title

      西日本皮膚科

      Volume: 74 Pages: 363-363

    • Related Report
      2013 Final Research Report
  • [Journal Article] 悪性黒色腫のダーモスコピー所見とtumor thicknessとの関連の検討2012

    • Author(s)
      柳瀬哲至,菅崇暢,河合幹雄,秀道広
    • Journal Title

      日本皮膚科学会雑誌

      Volume: 122 Pages: 1189-1189

    • Related Report
      2013 Final Research Report
  • [Journal Article] プロプラノロール内服療法が奏効した顔面の巨大なイチゴ状血管腫の1例2012

    • Author(s)
      田中 麻衣子, 柳瀬 哲至, 河合 幹雄, 信藤 肇, 秀 道広, 青木 恵美, 宮本 義洋, 唐川 修平
    • Journal Title

      西日本皮膚科

      Volume: 363 Pages: 363-363

    • Related Report
      2012 Research-status Report
  • [Journal Article] 下腹部に生じた皮下腫瘤2012

    • Author(s)
      高坂 卓馬, 信藤 肇, 秀 道広, 原 武, 行徳 英一, 西阪 隆
    • Journal Title

      日本皮膚病理組織学会会誌

      Volume: 27 Pages: 3-3

    • Related Report
      2012 Research-status Report
  • [Journal Article] 悪性黒色腫のダーモスコピー所見とtumor thicknessとの関連の検討2012

    • Author(s)
      柳瀬 哲至, 菅 崇暢, 河合 幹雄, 秀 道広
    • Journal Title

      日本皮膚科学会雑誌

      Volume: 122 Pages: 1189-1189

    • Related Report
      2012 Research-status Report
  • [Presentation] Visualization of individual cell activation by means of SPR imaging2013

    • Author(s)
      Yuhki Yanase, Tomoko Kawaguchi, Kaori Ishii, Michihiro Hide
    • Organizer
      7th International conference on molecularelectronics and bioekectronics
    • Place of Presentation
      Fukuoka-shi, Fukuoka
    • Year and Date
      2013-05-18
    • Related Report
      2013 Final Research Report
  • [Presentation] Application of SPR imaging for detection of individual living cell reaction and clinical diagnosis2013

    • Author(s)
      Michihiro HIDE
    • Organizer
      International Workshop on Nanodevice and Bio Systems Technologies 2013
    • Place of Presentation
      Higashihiroshima-shi, Hiroshima
    • Year and Date
      2013-04-03
    • Related Report
      2013 Final Research Report
  • [Presentation] 微量血液を利用したアレルギー診断のためのマルチウェルSPRIの開発2013

    • Author(s)
      柳瀬雄輝,川口智子,坂本憲児,秀道広
    • Organizer
      第60回応用物理学会春季学術講演会
    • Place of Presentation
      厚木市,神奈川県
    • Year and Date
      2013-03-29
    • Related Report
      2013 Final Research Report
  • [Presentation] Development of SPR imaging sensor for detection of individual living cell reactions and application for clinical diagnosis of type I allergy2013

    • Author(s)
      Yuhki Yanase, Takaaki Hiragun, Tomoko Kawaguchi, Kaori Ishii, Michihiro Hide
    • Organizer
      3rd International conference on Bio-sensing technology
    • Place of Presentation
      Sitges Spain
    • Related Report
      2013 Final Research Report
  • [Presentation] SPRによる好塩基球応答解析を利用したI型アレルギー診断法の開発2013

    • Author(s)
      柳瀬雄輝,石井香,川口智子,隅崎修孝,小原隆之,平郡隆明,秀道広
    • Organizer
      第26回バイオメディカル分析科学シンポジウム
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] マルチウェルSPRイメージングによる即時型アレルギー診断法の開発2013

    • Author(s)
      柳瀬雄輝,石井香,川口智子,隈崎修孝,小原隆之,平郡隆明,秀道広
    • Organizer
      第63回日本アレルギー学会秋季学術大会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Surface plasmon resonance for cell-based clinical diagnosis2013

    • Author(s)
      Hide M
    • Organizer
      5th Hiroshima Conferenence on Education and Science in Dentistry
    • Place of Presentation
      東広島
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Application of SPR imaging for detection of individual living cell reaction and clinical diagnosis2013

    • Author(s)
      Hide M
    • Organizer
      International Workshop on Nanodevice and Bio Systems Technologies 2013
    • Place of Presentation
      東広島 日本
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Development of SPR imaging sensor for detection of individual living cell reactions and application for clinical diagnosis of type I allergy2013

    • Author(s)
      Yuhki Yanase, Takaaki Hiragun, Tomoko Kawaguchi, Kaori Ishii, Michihiro Hide
    • Organizer
      3rd International conference on Bio-sensing technology
    • Place of Presentation
      Sitges、スペイン
    • Related Report
      2013 Annual Research Report
  • [Presentation] SPRによる好塩基球応答解析を利用したI型アレルギー診断法の開発2013

    • Author(s)
      柳瀬雄輝 石井 香、川口智子、隈崎修孝、小原隆之、平郡隆明、秀 道広
    • Organizer
      第26回バイオメディカル分析科学シンポジウム
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] Visualization of individual cell activation by means of SPR imaging2013

    • Author(s)
      Yuhki Yanase, Tomoko Kawaguchi, Kaori Ishii, Michihiro Hide
    • Organizer
      7th International conference on molecularelectronics and bioekectronics
    • Place of Presentation
      Fukuoka, Japan
    • Related Report
      2012 Research-status Report
  • [Presentation] 微量血液を利用したアレルギー診断のためのマルチウェルSPRIの開発2013

    • Author(s)
      柳瀬 雄輝、川口智子、坂本憲児、秀 道広
    • Organizer
      第60回応用物理学会春季学術講演会
    • Place of Presentation
      厚木市
    • Related Report
      2012 Research-status Report
  • [Presentation] Visualization of human basophil activation by means of surface plasmon resonance imaging2012

    • Author(s)
      Yuhki Yanase, Takaaki Hiragun, Tomoko Kawaguchi, Kaori Ishii, Michihiro Hide
    • Organizer
      Label free technologies 2012
    • Place of Presentation
      Amsterdam The Netherlands
    • Year and Date
      2012-11-01
    • Related Report
      2013 Final Research Report
  • [Presentation] 当科における高齢者の皮膚がんに対する治療の現状2012

    • Author(s)
      栁瀬哲至,菅崇暢,河合幹雄,秀道広
    • Organizer
      第64回日本皮膚科学会西部支部学術大会
    • Place of Presentation
      広島市,広島県
    • Year and Date
      2012-10-28
    • Related Report
      2013 Final Research Report
  • [Presentation] Surface plasmon resonance for cell-based diagnosis2012

    • Author(s)
      Michihiro HIDE
    • Organizer
      5th Hiroshima Conferenence on Education and Science in Dentistry
    • Place of Presentation
      Higashihiroshima-shi, Hiroshima
    • Year and Date
      2012-10-12
    • Related Report
      2013 Final Research Report
  • [Presentation] 80歳以上の患者における血管肉腫の治療経験2012

    • Author(s)
      菅崇暢,栁瀬哲至,河合幹雄,秀道広
    • Organizer
      第28回日本皮膚悪性腫瘍学会学術大会
    • Place of Presentation
      札幌市,北海道
    • Year and Date
      2012-06-30
    • Related Report
      2013 Final Research Report
  • [Presentation] 上口唇から口腔粘膜まで広範囲に散在する悪性黒色腫に対しferon療法が奏効した1例2012

    • Author(s)
      栁瀬哲至,菅崇暢,河合幹雄,秀道広
    • Organizer
      第28回日本皮膚悪性腫瘍学会学術大会
    • Place of Presentation
      札幌市,北海道
    • Year and Date
      2012-06-30
    • Related Report
      2013 Final Research Report
  • [Presentation] Application of SPR imaging for detection of individual cell activation2012

    • Author(s)
      Yuhki Yanase, Tomoko Kawaguchi, Kaori Ishii, Kenji Sakamoto, Ryo Miyake, Michihiro Hide
    • Organizer
      ISMM2012
    • Place of Presentation
      Hsinchu Taiwan
    • Year and Date
      2012-06-12
    • Related Report
      2013 Final Research Report
  • [Presentation] Application of SPR imaging for detection of individual cell reaction and clinical diagnosis of allergy2012

    • Author(s)
      Yuhki Yanase, Tomoko Kawaguchi, Kaori Ishii, Kenji Sakamoto, Ryo Miyake, Michihiro Hide
    • Organizer
      Biosensors 2012
    • Place of Presentation
      Cancun Mexico
    • Year and Date
      2012-05-18
    • Related Report
      2013 Final Research Report
  • [Presentation] Application of SPR imaging for detection of individual cell reaction and clinical diagnosis of allergy2012

    • Author(s)
      Yuhki Yanase, Tomoko Kawaguchi, Kaori Ishii, Kenji Sakamoto, Ryo Miyake, Michihiro Hide
    • Organizer
      Biosensors 2012
    • Place of Presentation
      Cancun, Mexico
    • Related Report
      2012 Research-status Report
  • [Presentation] Application of SPR imaging for detection of individual cell activation2012

    • Author(s)
      Yuhki Yanase, Tomoko Kawaguchi, Kaori Ishii, Kenji Sakamoto, Ryo Miyake, Michihiro Hide
    • Organizer
      ISMM2012
    • Place of Presentation
      Hsinchu, Taiwan
    • Related Report
      2012 Research-status Report
  • [Presentation] Visualization of human basophil activation by means of surface plasmon resonance imaging2012

    • Author(s)
      Yuhki Yanase, Takaaki Hiragun, Tomoko Kawaguchi, Kaori Ishii, Michihiro Hide
    • Organizer
      Label free technologies 2012
    • Place of Presentation
      Amsterdam, The Netherlands
    • Related Report
      2012 Research-status Report
  • [Presentation] 80歳以上の患者における血管肉腫の治療経験2012

    • Author(s)
      菅 崇暢、栁瀬哲至、河合幹雄、秀 道広
    • Organizer
      第28回日本皮膚悪性腫瘍学会学術大会
    • Place of Presentation
      札幌市
    • Related Report
      2012 Research-status Report
  • [Presentation] 上口唇から口腔粘膜まで広範囲に散在する悪性黒色腫に対しferon療法が奏効した1例2012

    • Author(s)
      栁瀬哲至、菅 崇暢、河合幹雄、秀 道広
    • Organizer
      第28回日本皮膚悪性腫瘍学会学術大会
    • Place of Presentation
      札幌市
    • Related Report
      2012 Research-status Report
  • [Presentation] 当科における高齢者の皮膚がんに対する治療の現状2012

    • Author(s)
      栁瀬哲至、菅 崇暢、河合幹雄、秀 道広
    • Organizer
      第64回日本皮膚科学会西部支部学術大会
    • Place of Presentation
      広島市
    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] マルチウェル解析方法および刺激用マルチウェルチャンバチップ2013

    • Inventor(s)
      秀 道広、柳 瀬雄輝、石井 香、川口智子
    • Industrial Property Rights Holder
      広島大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-159020
    • Filing Date
      2013-07-31
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] マルチウェル解析方法および刺激用マルチウェルチャンバチップ2013

    • Inventor(s)
      秀 道広、柳瀬雄輝、石井 香、川口智子
    • Industrial Property Rights Holder
      広島大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-159020
    • Filing Date
      2013-07-31
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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