| Project/Area Number |
24659531
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | Hiroshima University |
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Principal Investigator |
HIDE Michihiro 広島大学, 医歯薬保健学研究院, 教授 (50284188)
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| Co-Investigator(Renkei-kenkyūsha) |
YANASE Yuhki 広島大学, 医歯薬保健学研究院, 助教 (40452586)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 血管肉腫 / 皮膚悪性腫瘍 / バイオセンサー / 表面プラズモン共鳴 / 血管内皮細胞 / 細胞内情報伝達 |
|---|
| Research Abstract |
We applied surface plasmon resonance (SPR) for the analysis of circulating tumor cells (CTC) derived from angiosarcoma. We could recover up to 10 angiosarcoma cells from a mixture of 1 ml of human peripheral blood, mixed with 2,000 angiosarcoma cells and anti-CD146 antibodies. Both angiosarcoma cells and normal vascular endothelial cells expressed VEGF receptors on their surface, and one of two angiosarcoma cell lines phosphorylated their EVGF receptors in response to VEGF. However, only normal endothelial cells showed an SPR signal by VEGF. These results suggest that SPR biosensor may detect CTC of angiosarcoma both sensitively and rapidly.
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