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Comprehensive identification of the Pet-1-target genes by chromatin immunoprecipitation assays in RN46A cells derived from the rat raphe nucleus.

Research Project

Project/Area Number 24659548
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Psychiatric science
Research InstitutionSt. Marianna University School of Medicine

Principal Investigator

MATSUI Hiroaki  聖マリアンナ医科大学, 医学(系)研究科(研究院), 教授 (90181685)

Research Collaborator NAWA Yukino  聖マリアンナ医科大学, 医学(系)研究科(研究院), 助教 (10549786)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsセロトニン神経系 / クロマチン免疫沈降法 / ラット縫線核由来RN46A細胞 / セロトニン神経細胞特異的転写因子Pet-1 / Pet-1標的遺伝子 / 神経特異的抑制因子 / 転写因子 / 遺伝子発現 / クロマチン沈降法
Research Abstract

To identify Pet-1 (serotonergic neuron-specific transcription factor)-binding sites in rat raphe nucleus-derived RN46A cells, chromatin-immunoprecipitation (ChIP) assays were performed. It was revealed that Pet-1 binding was inhibited through the neuron-restrictive silencer factor(NRSF)-NRSF binding element(NRSE) system or the potential repressor proteins independent of NRSF. Additionally, the detection of Pet-1 binding to target genes was occasionally difficult even under conditions where Pet-1 was overexpressed. Taken together, to exclude the function of repressor proteins including NRSF may be necessary to identify the Pet-1 binding to its target genes by ChIP assays in RN46A cells. To overcome these difficulties should be required to use RN46A cells as a proper model of central serotonergic neurons and characterize the Pet-1-mediated transcriptional network in serotonergic neurons.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (8 results)

All 2013 2012 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (6 results) Remarks (1 results)

  • [Journal Article] Tryptophan hydroxylase 2 (TPH2) in a neuronal cell line : modulation by cell differentiation and NRSF/ rest activity2012

    • Author(s)
      Gentile MT, Nawa Y, Lunardi G, Florio T, Matsui H, Colucci-D'Amato L
    • Journal Title

      Journal of Neurochemistry

      Volume: Vol. 123、No.6 Issue: 6 Pages: 963-970

    • DOI

      10.1111/jnc.12004

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] ヒトセロトニン4受容体遺伝子発現調節機構 : ERG、EGRおよびNF1による調節2013

    • Author(s)
      那和雪乃
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2013-12-04
    • Related Report
      2013 Final Research Report
  • [Presentation] Transcriptional regulation of the human serotonin type 4(5-ht4) receptor gene : identification and characterization of novel promoter and enhancer2013

    • Author(s)
      Nawa Y
    • Organizer
      Annual Meeting Neuroscience 2013
    • Place of Presentation
      San Diego、U.S.A
    • Year and Date
      2013-11-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Transcriptional regulation of the human serotonin type 4(5-ht4) receptor gene:identification and characterization of novel promoter and enhancer2013

    • Author(s)
      Yukino Nawa, Masanori Ootaki, Rie Kuwabara, Tomoko Hiroi, Ryoya Takahashi, Hiroaki Matsui
    • Organizer
      北米神経科学会
    • Place of Presentation
      サンディエゴ(米国)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ヒトセロトニン4受容体遺伝子発現調節機構:ERG、EGRおよびNF1による調節2013

    • Author(s)
      那和雪乃、大滝正則、桑原理恵、廣井朋子、高橋良哉、松井宏晃
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Transcriptional regulation of the human tryptophan hydroxylase-2 gene by Lmx1b in RN46A cells2012

    • Author(s)
      Nawa Y
    • Organizer
      Annual Meeting Neuroscience 2012
    • Place of Presentation
      New Orleans、U.S.A
    • Year and Date
      2012-10-14
    • Related Report
      2013 Final Research Report
  • [Presentation] Transcriptional regulation of the human tryptophan hydroxylase-2 gene by Lmx1b in RN46A cells2012

    • Author(s)
      Yukino Nawa
    • Organizer
      北米神経科学会
    • Place of Presentation
      ニューオリンズ(米国)
    • Related Report
      2012 Research-status Report
  • [Remarks] 聖マリアンナ医科大学大学院脳情報制御医学分野

    • URL

      http://www.marianna-u.ac.jp/isotope/facilities/12541/012540.html

    • Related Report
      2013 Final Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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