| Project/Area Number |
24659548
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Psychiatric science
|
|---|
| Research Institution | St. Marianna University School of Medicine |
|---|
Principal Investigator |
MATSUI Hiroaki 聖マリアンナ医科大学, 医学(系)研究科(研究院), 教授 (90181685)
|
|---|
| Research Collaborator |
NAWA Yukino 聖マリアンナ医科大学, 医学(系)研究科(研究院), 助教 (10549786)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | セロトニン神経系 / クロマチン免疫沈降法 / ラット縫線核由来RN46A細胞 / セロトニン神経細胞特異的転写因子Pet-1 / Pet-1標的遺伝子 / 神経特異的抑制因子 / 転写因子 / 遺伝子発現 / クロマチン沈降法 |
|---|
| Research Abstract |
To identify Pet-1 (serotonergic neuron-specific transcription factor)-binding sites in rat raphe nucleus-derived RN46A cells, chromatin-immunoprecipitation (ChIP) assays were performed. It was revealed that Pet-1 binding was inhibited through the neuron-restrictive silencer factor(NRSF)-NRSF binding element(NRSE) system or the potential repressor proteins independent of NRSF. Additionally, the detection of Pet-1 binding to target genes was occasionally difficult even under conditions where Pet-1 was overexpressed. Taken together, to exclude the function of repressor proteins including NRSF may be necessary to identify the Pet-1 binding to its target genes by ChIP assays in RN46A cells. To overcome these difficulties should be required to use RN46A cells as a proper model of central serotonergic neurons and characterize the Pet-1-mediated transcriptional network in serotonergic neurons.
|
