Development of in vitro culture for peripheral B cell differentiation into plasma cell
| Project/Area Number |
24659587
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Nagoya University |
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Principal Investigator |
KOBAYASHI Takaaki 名古屋大学, 医学(系)研究科(研究院), 寄附講座教授 (70314010)
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| Co-Investigator(Kenkyū-buntansha) |
HANEDA Masataka 名古屋大学, 大学院医学系研究科, 寄附講座講師 (50436995)
IWASAKI Kenta 名古屋大学, 大学院医学系研究科, 寄附講座講師 (10508881)
MIWA Yuko 名古屋大学, 大学院医学系研究科, 研究員 (90572941)
KUZUYA Takafumi 名古屋大学, 医学部附属病院, 薬剤部副長 (00444406)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 移植・再生医療 / Ｂリンパ球 / 形質細胞 / 培養 / HLA抗体 / 抗体 |
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| Research Abstract |
We attempted to develop a novel in vitro culture assay for proliferation and differentiation of peripheral B cells into plasma cells. Using 3 step culture method, the difference in action points (in the process of B cell differentiation) between clinical available immunosuppressive agents such as calcineurin inhibitor (CNI), mTOR-inhibitor (mTOR-I), anti-metabolite and steroid was clarified. The results showed anti-metabolite, mTOR-I and steroid could suppress B cells in the early, middle and late phase, respectively. Effective method for HLA antibody secretion in the B cell culture system has been established using APRIL (A proliferation-inducing ligand), BAFF (B cell activating factor), mitogen-stimulated T cell secretion and adipose-derived mesenchymal stem cells. We can move on to the next step to evaluate early diagnosis of chronic antibody mediated rejection.
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Report
(3 results)
Research Products
(37 results)
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[Journal Article] Frequent development of subclinical chronic antibody-mediated rejection within 1 year after renal transplantation with pre-transplant positive donor-specific antibodies and negative CDC crossmatches2013
Author(s)
Shigeyoshi Yamanaga,Yoshihiko Watarai,Takayuki Yamamoto,Makoto Tsujita,Takahisa Hiramitsu,Koji Nanmoku,Norihiko Goto,Asami Takeda,Kunio Morozumi,Akio Katayama,Hiroh Saji,Kazuharu Uchida,and Takaaki Kobayashi
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Journal Title
Human Immunology
Volume: 74巻
Issue: 9
Pages: 1111-1118
DOI
Related Report
Peer Reviewed
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[Journal Article] Comparative study on signal transduction in endothelial cells after anti-A/B and HLA antibody reaction : implication of accommodation2012
Author(s)
Iwasaki K, Miwa Y, Ogawa H, Iwamoto M, Furusawa T, Onishi A, Kuzuya T, Haneda M, Watarai Y, Uchida K, Kobayashi T.
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Journal Title
Transplantation
Volume: 93
Issue: 4
Pages: 390-397
DOI
Related Report
Peer Reviewed
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[Journal Article] Significance of C4d deposition in antibody-mediated rejection2012
Author(s)
Takeda A,Otsuka Y,Horike K,Inaguma D,Hiramitsu T,Yamamoto T,Nanmoku K,Goto N,Watarai Y,Uchida K,Morozumi K,Kobayashi T
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Journal Title
Clin Transplantation
Volume: 26 (Supple 24)巻
Pages: 43-48
Related Report
Peer Reviewed
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[Presentation] No Inhibitory Effect of Pre-Transplant Desensitization Treatment with Rituximab or Splenectomy on de Novo HLA Antibody Production in Renal Transplantation2012
Author(s)
Kobayashi T,Yamamoto T,Kuroki K,Hiramitsu T,Tsujita M,Goto N,Nanmoku K,Nagasaka T,Ohtsuka S,Katayama A,Watarai Y,Takeda A,Morozumi K,Uchida K
Organizer
24th International Congress of The Transplantation Society
Place of Presentation
Berlin (Germany)
Related Report
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