| Project/Area Number |
24659587
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Nagoya University |
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Principal Investigator |
KOBAYASHI Takaaki 名古屋大学, 医学(系)研究科(研究院), 寄附講座教授 (70314010)
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| Co-Investigator(Kenkyū-buntansha) |
HANEDA Masataka 名古屋大学, 大学院医学系研究科, 寄附講座講師 (50436995)
IWASAKI Kenta 名古屋大学, 大学院医学系研究科, 寄附講座講師 (10508881)
MIWA Yuko 名古屋大学, 大学院医学系研究科, 研究員 (90572941)
KUZUYA Takafumi 名古屋大学, 医学部附属病院, 薬剤部副長 (00444406)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 移植・再生医療 / Ｂリンパ球 / 形質細胞 / 培養 / HLA抗体 / 抗体 |
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| Research Abstract |
We attempted to develop a novel in vitro culture assay for proliferation and differentiation of peripheral B cells into plasma cells. Using 3 step culture method, the difference in action points (in the process of B cell differentiation) between clinical available immunosuppressive agents such as calcineurin inhibitor (CNI), mTOR-inhibitor (mTOR-I), anti-metabolite and steroid was clarified. The results showed anti-metabolite, mTOR-I and steroid could suppress B cells in the early, middle and late phase, respectively. Effective method for HLA antibody secretion in the B cell culture system has been established using APRIL (A proliferation-inducing ligand), BAFF (B cell activating factor), mitogen-stimulated T cell secretion and adipose-derived mesenchymal stem cells. We can move on to the next step to evaluate early diagnosis of chronic antibody mediated rejection.
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