| Project/Area Number |
24659588
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Mie University |
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Principal Investigator |
KUSUNOKI Masato 三重大学, 医学(系)研究科(研究院), 教授 (50192026)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Koji 三重大学, 医学部附属病院, 講師 (10345986)
INOUE Yasuhiro 三重大学, 医学部附属病院, 講師 (20324535)
UCIDA Keiichi 三重大学, 医学部附属病院, 准教授 (30293781)
OKUGAWA Yoshinaga 三重大学, 医学部附属病院, 助教 (30555545)
ARAKI Toshimitu 三重大学, 医学部附属病院, 講師 (70343217)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 二光子レーザー顕微鏡 / 炎症性腸疾患 / 生体内イメージング / 二光子励起顕微鏡 / 炎症性腸疾患モデル / Infliximab / infliximab |
|---|
| Research Abstract |
We attempted to visualize in vivo therapeutic response of infliximab (IFX) and tacrolimus (FK506) in the murine IBD model using two photon laser scanning microscopy and to analyze their histopathological response morphologically.
The number of leukocyte infiltrates within the crypt and the number of crypt loss were decreased in IFX-treated mice. The length of colonic crypt was increased in IFX-treated mice. Serial change in regenerative epithelial cells was not observed. The findings in FK506-treated mice were similar to those in IFX-treated mice. The fluorescence labeled anti-TNF-alpha antibody was observed in the mucosal stroma, suggesting the binding of labeled antibodies with free TNF-alpha. It was also observed in the surface of leukocytes within the postcapillary venules or those infiltrating into the damaged crypt, suggesting the binding of labeled antibodies with the membrane binding TNF- alpha of the surface TNF- alpha producing leukocytes.
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