Developing a novel vaccine therapy targeting cancer stem cells
Project/Area Number |
24659592
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
General surgery
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Research Institution | Sapporo Medical University |
Principal Investigator |
HIRATA Koichi 札幌医科大学, 医学部, 教授 (50136959)
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Co-Investigator(Kenkyū-buntansha) |
SATO Noriyuki 札幌医科大学, 医学部, 教授 (50158937)
TORIGOE Toshihiko 札幌医科大学, 医学部, 准教授 (20301400)
TAMURA Yasuaki 北海道大学, 先端生命科学研究院, 特任教授 (80322329)
HIROHASHI Yoshihiko 札幌医科大学, 医学部, 助教 (30516901)
MIZUGUCHI Toru 札幌医科大学, 医学部, 准教授 (30347174)
KIMURA Yasutoshi 札幌医科大学, 医学部, 准教授 (80311893)
KUTOMI Goro 札幌医科大学, 医学部, 講師 (10404625)
OGAWA Tadashi 札幌医科大学, 医学部, 研究員 (70516658)
KOUNO Tsuyoshi 札幌医科大学, 医学部, 研究員 (50516664)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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Keywords | 癌 / 膵 / 免疫 / 幹細胞 / side population |
Outline of Final Research Achievements |
Pancreatic cancer is known as high mortality disease compared with other cancers due to higher drug resistance and invasive and metastatic potentials. We started to research for developing new treatments that kill pancreatic CSCs selectively by immunological approaches such as cancer vaccines. In past we reported that we identified a CSC specific gene OR7C1 from colorectal cancers, so we thought OR7C1 may be a CSC specific gene in pancreatic cancer, too. At first, we isolated pancreatic CSCs by sphere culture method and analyze expression levels. Sphere culture cell showed predominantly higher expression of OR7C1 and this suggested that OR7C1 may be a good target for CSC specific therapy. Next, we isolated OR7C1 high expression (OR7C1high) cells by flowcytometry and try to analyze about stemness. But analyses did not work well because we could not expand OR7C1high cells due to cell death and finally we could not identify CSC specific peptides in 3 years.
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Report
(4 results)
Research Products
(43 results)
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[Journal Article] Phase I Clinical Study of Survivin-Derived Peptide Vaccine for Patients with Advanced Gastrointestinal Cancers.2015
Author(s)
Satomi F, Shima H, Mizuguchi T, Torigoe T, Kutomi G, Kimura Y, Hirohashi Y, Tamura Y, Tsukahara T, Kanaseki T, Takahashi A, Asanuma H, Ito Y, Hayashi H, Sugita O, Sato N and Hirata K.
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Journal Title
Int J Cancer Clin Res
Volume: 2
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] A dose-escalation study of oxaliplatin/capecitabine/irinotecan (XELOXIRI) and bevacizumab as a first-line therapy for patients with metastatic colorectal cancer.2015
Author(s)
Sato Y, Ohnuma H, Hirakawa M, Takahashi M, Osuga T, Okagawa Y, Murase K, Takada K, Kawano Y, Iyama S, Hayashi T, Sato T, Miyanishi K, Takimoto R, Kobune M, Okita K, Mizuguchi T, Furuhata T, Hirata K, Kato J
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Journal Title
Cancer Chemother Pharmacol
Volume: 75
Issue: 3
Pages: 587-597
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Trials of vaccines for pancreatic ductal adenocarcinoma: Is there any hope of an improved prognosis?2015
Author(s)
Mizuguchi T, Torigoe T, Satomi F, Shima H, Kutomi G, Ota S, Ishii M, Hayashi H, Asakura S, Hirohashi Y, Meguro M, Kimura Y, Nishidate T, Okita K, Ishino M, Miyamoto A, Hatakenaka M, Sato N, Hirata K.
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Journal Title
Surg Today
Volume: in press
Issue: 2
Pages: 139-148
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Heat shock protein DNAJB8 is a novel target for immunotherapy of colon cancer-initiating cells.2014
Author(s)
Morita R, Nishizawa S, Torigoe T, Takahashi A, Tamura Y, Tsukahara T, Kanaseki T, Sokolovskaya A, Kochin V, Kondo T, Hashino S, Asaka M, Hara I, Hirohashi Y, Sato N.
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Journal Title
Cancer Sci
Volume: 105
Issue: 4
Pages: 389-395
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Specific targeting of a naturally presented osteosarcoma antigen, papillomavirus binding factor peptide, using an artificial monoclonal antibody.2014
Author(s)
Tsukahara T, Emori M, Murata K, Hirano T, Muroi N, Kyono M, Toji S, Watanabe K, Torigoe T, Kochin V, Asanuma H, Matsumiya H, Yamashita K, Himi T, Ichimiya S, Wada T, Yamashita T, Hasegawa T, Sato N.
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Journal Title
J Biol Chem
Volume: 289
Issue: 32
Pages: 22035-22047
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Fibroblasts induce expression of FGF4 in ovarian cancer stem-like cells/cancer-initiating cells and upregulate their tumor initiation capacity.2014
Author(s)
Yasuda K, Torigoe T, Mariya T, Asano T, Kuroda T, Matsuzaki J, Ikeda K, Yamauchi M, Emori M, Asanuma H, Hasegawa T, Saito T, Hirohashi Y, Sato N.
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Journal Title
Lab Invest
Volume: 94
Issue: 12
Pages: 1355-69
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Human Endoplasmic Reticulum Oxidoreductin 1-α(hERO1-α) is a Novel Predictor for Poor Prognosis of Breast Cancer2013
Author(s)
Kutomi G, Tamura Y, Tanaka T, Kajiwara T, Kukita K, Ohmura T, Shima H, Takamaru T, Satomi F, Suzuki Y, Torigoe T, Sato N, Hirata H
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Journal Title
Cancer Sci.
Volume: 104
Issue: 8
Pages: 1091-6
DOI
Related Report
Peer Reviewed
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[Journal Article] Expression and function of FERMT genes in colon carcinoma cells.2013
Author(s)
Kiriyama K, Hirohashi Y, Torigoe T, Kubo T, Tamura Y, Kanaseki T, Takahashi A, Nakazawa E, Saka E, Ragnarsson C, Nakatsugawa M, Inoda S, Asanuma H, Takasu H, Hasegawa T, Yasoshima T, Hirata K, Sato N
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Journal Title
Anticancer Res
Volume: 33
Pages: 167-73
Related Report
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[Journal Article] Immunotherapeutic benefit ofα-interferon (IFNα) in survivin2B-derived peptide vaccination for advanced pancreatic cancer patients2013
Author(s)
Kameshima H, Tsuruma T, Kutomi G, Shima H, Iwayama Y, Kimura Y, Imamura M, Torigoe T, Takahashi A, Hirohashi Y, Tamura Y, Tsukahara T, Kanaseki T, Sato N, Hirata K
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Journal Title
Cancer Sci.
Volume: 104
Issue: 1
Pages: 124-129
DOI
Related Report
Peer Reviewed
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[Journal Article] Treatment of pancreatic fibrosis with siRNA against a collagen-specific chaperone in vitamin A-coupled liposomes.2013
Author(s)
Ishiwatari H, Sato Y, Murase K, Yoneda A, Fujita R, Nishita H, Birukawa NK, Hayashi T, Sato T, Miyanishi K, Takimoto R, Kobune M, Ota S, Kimura Y, Hirata K, Kato J, Niitsu Y.
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Journal Title
Gut
Volume: 62(9)
Issue: 9
Pages: 1328-39
DOI
Related Report
Peer Reviewed
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[Journal Article] Heat shock enhances the expression of cytotoxic granule proteins and augments the activities of tumor-associated antigen-specific cytotoxic T lymphocytes2012
Author(s)
Takahashi A, Torigoe T, Tamura Y, Kanaseki T, Tsukahara T, Sasaki Y, Kameshima H, Tsuruma T, Hirata K, Tokino T, Hirohashi Y, Sato N
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Journal Title
Cell Stress Chaperones
Volume: 17
Issue: 6
Pages: 757-763
DOI
Related Report
Peer Reviewed
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[Journal Article] Establishment of a monoclonal anti-pan HLA class I antibody suitable for immunostaining of formalin-fixed tissue : Unusually high frequency of down-regulation in breast cancer tissues2012
Author(s)
Torigoe, T., Asanuma, H., Nakazawa. E., Tamura, Y., Hirohashi, Y., Yamamoto, E., Kanaseki, T., Hasegawa, T., Sato, N.
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Journal Title
Pathol Int
Volume: 62
Issue: 5
Pages: 303-8
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Efficiency of G2/M-related tumor-associated antigen-targeting cancer immunotherapy depends on antigen expression in the cancer stem-like population2012
Author(s)
Takashi Mori, Satoshi Nishizawa, Yoshihiko Hirohashi, Toshihiko Torigoe, Yasuaki Tamura, Akari Takahashi, Vitaly Kochin, Reona Fujii, Kondo Toru, Mark I. Greene, Isao Hara, Noriyuki Sato
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Journal Title
Experimental and Molecular Pathology
Volume: 92
Issue: 1
Pages: 27-32
DOI
Related Report
Peer Reviewed
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