| Project/Area Number |
24659597
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
General surgery
|
|---|
| Research Institution | National Cardiovascular Center Research Institute |
|---|
Principal Investigator |
NAKATANI Takeshi 独立行政法人国立循環器病研究センター, 病院, 部長 (60155752)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YAMAOKA Tetsuji 独立行政法人国立循環器病研究センター, 研究所, 部長 (50243126)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
|---|
| Keywords | 人工臓器学 / 心筋梗塞 / 移植・再生医療 / 人工臓器 |
|---|
| Research Abstract |
Stem cell-based myocardial regeneration has been emerged as alternative strategy for the heart transplantation or destination therapy using left ventricular assist system (LVAS). Especially self-beating cardiomyocytes are promising cells, but effective protocols to prepare the mature cardiomyocytes from various stem cells have not been clarified yet. The purpose of this article is find out the effect of stem cell culture niche (ECM proteins and substrate elasticities) on the cardiac differentiation, beating induction, and beating duration separately by using iPS cells (iPSs) and neonatal cardiomyocytes (NCMs). The cardiac differentiation step was greatly enhanced on Col-I-gel but the beating-induction step (a-MHC, TnC1, TnT2 expression) was effective on FN-culture dishes. The beating-duration step, which is assessed by counting the number of beating colonies of NCMs, preferred Col-I–gel. We also found the effective surface for keeping the undifferentiated stages of iPS cells.
|
