Cancer stem cell for peritoneal metastasis in abdominal cavity
| Project/Area Number |
24659600
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KITAYAMA Joji 東京大学, 医学部付属病院, 准教授 (20251308)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAGUCHI Hironori 東京大学, 医学部付属病院, 助教 (20376445)
YAMASHITA Hiroharu 東京大学, 医学部付属病院, 助教 (50599397)
YAZAWA Kentaro 東京大学, 医学部付属病院, 助教 (60572542)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 腹膜播種 / 幹細胞 / フローサイトメトリー / 腹腔 / FACS |
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| Research Abstract |
We found that a minor subset of human intraperitoneal free cells with CD90+CD45- displayed the characteristics of mesenchymal stem cell, and differentiated into myofibroblast by the stimulation with TGFb, and intraperitoneal coinjection of with MKN45 significantly enhanced the rate of metastatic formation in the peritoneum of nude mice. Histological examination revealed that many MSC were engrafted in metastatic nodules and were mainly located at the fibrous area. Dasatinib, a tyrosine kinase inhibitor, strongly inhibited the proliferation of MSC but not MKN45 in vitro. Nevertheless, oral administration of Dasatinib significantly inhibited the development of peritoneal metastasis of MKN45, and resulted in reduced fibrillar formation of metastatic nodules. The rate of CD326+ tumor cells measured with FACS could be a reliable diagnostic biomarker to determine the severity of peritoneal metastasis as well as effectiveness of chemotherapy.
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Report
(3 results)
Research Products
(12 results)
