| Project/Area Number |
24659606
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Kyoto University |
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Principal Investigator |
UEMOTO Shinji 京都大学, 大学院・医学研究科・肝胆膵移植外科, 教授 (40252449)
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| Co-Investigator(Kenkyū-buntansha) |
ISHII Takamichi 京都大学, 医学(系)研究科, 助教 (70456789)
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| Project Period (FY) |
2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | 再生医学 / 癌幹細胞 / 肝臓癌 |
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| Research Abstract |
We investigated to the origin of CSCs and the oncogenic process of HCC. First, we successfully generated oncogene-regulatory expressing transgenic micewhich express the fusion protein of c-Myc and ER. We isolated fetal hepatic progenitor cells from the transgenic fetal mice by the method of cell aggregate which we established previously. And then, we isolated adult hepatocytes from adult transgenic mice. However, it is difficult for us to breed the transgenic mice, so we actually cannot use a needed amount of samples.We are trying to breed and increase the transgenic mice.After that, we will expose hepatocytes at each stage of differentiation to Tamoxifen and acquire cancer cells at several stages.
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