Identification and characterization of hepatic cancer stem cells and cell biological investigation of oncologic process for developing new treatment of HCC
| Project/Area Number |
24659606
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Kyoto University |
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Principal Investigator |
UEMOTO Shinji 京都大学, 大学院・医学研究科・肝胆膵移植外科, 教授 (40252449)
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| Co-Investigator(Kenkyū-buntansha) |
ISHII Takamichi 京都大学, 医学(系)研究科, 助教 (70456789)
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| Project Period (FY) |
2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | 再生医学 / 癌幹細胞 / 肝臓癌 |
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| Research Abstract |
We investigated to the origin of CSCs and the oncogenic process of HCC. First, we successfully generated oncogene-regulatory expressing transgenic micewhich express the fusion protein of c-Myc and ER. We isolated fetal hepatic progenitor cells from the transgenic fetal mice by the method of cell aggregate which we established previously. And then, we isolated adult hepatocytes from adult transgenic mice. However, it is difficult for us to breed the transgenic mice, so we actually cannot use a needed amount of samples.We are trying to breed and increase the transgenic mice.After that, we will expose hepatocytes at each stage of differentiation to Tamoxifen and acquire cancer cells at several stages.
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Report
(2 results)
Research Products
(7 results)
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[Journal Article] Comparative study of transplantation of hepatocytes at various differentiation stages into mice with lethal liver damage2012
Author(s)
Ryo Kamimura, Takamichi Ishii, Naoya Sasaki, Masatoshi Kajiwara, Takafumi Machimoto, Miwa Saito, Kohno K, Hirofumi Suemori, Norio Nakatsuji, Iwao Ikai, Kentaro Yasuchik, Shinji Uemoto
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Journal Title
Cell Transplant
Volume: 21(11)
Issue: 11
Pages: 2351-62
DOI
Related Report
Peer Reviewed
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[Presentation] 大量肝切除後の急性肝不全に対する、肝細胞自家移植を用いた治療戦略2012
Author(s)
喜多貞彦,石井隆道,小木曾 聡,片山外大,河合隆之,安田勝太郎,上村 良,梶原正俊,波多野悦朗,落合孝広,上本伸二
Organizer
第39回日本臓器保存生物医学会
Place of Presentation
福島
Year and Date
2012-11-17
Related Report
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[Book]2013
Author(s)
Takamichi Ishii, Kentaro Yasuchika, Iwao Ikai
Publisher
Hepatic differentiation of embryonic stem cells by murine fetal liver mesenchymal cells
Related Report
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