| Project/Area Number |
24659610
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Digestive surgery
|
|---|
| Research Institution | Yamaguchi University |
|---|
Principal Investigator |
OKA MASAAKI 山口大学, 医学(系)研究科(研究院), 教授 (70144946)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TSUNEDOMI Ryouichi 山口大学, 大学院医学系研究科, 助教 (10420514)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | 肝癌 / 癌幹細胞 / 肝細胞癌 |
|---|
| Research Abstract |
The possible existence of plasticity between cancer stem cells and their more differentiated derivative cancer cells was suggested. We successfully induced cancer stem-like sphere cells from human HCC cell lines with specified medium. Poorly differentiated HCC derived SK-HEP-1 and undifferentiated HCC derived HLE cell lines efficiently formed spheres (SK-sphere and HLE-sphere). In the presence of several anti-cancer drugs except sorafenib, the cell viability of SK-sphere and HLE-sphere were significantly higher than that of parental cells. SK-sphere showed higher HIF1A mRNA expression, more CD44 variant-positive cells, and lower ROS production compared to parental cells. Integrated analysis showed that HIF1A and its downstream genes were up-regulated in SK-sphere. Finally, e identified several new marker and therapy target candidates (mRNA, microRNA, and protein) to cancer stem cells by integrated omics analysis.
|
