Relationship between microsatellite instability and microRNA in colon cancer

• Project/Area Number: 24659619
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery
• Research Institution: Fukushima Medical University
• Principal Investigator: NAKAMURA IZUMI 福島県立医科大学, 医学部, 准教授 (80423804)
• Co-Investigator(Kenkyū-buntansha): OHKI Shinji 福島県立医科大学, 器官制御外科, 准教授 (20381361) ; TAKENOSHITA Seiichi 福島県立医科大学, 器官制御外科, 教授 (10167489) ; 齋藤 元伸 福島県立医科大学, 医学部, 研究員 (90611749)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: MSI / 大腸癌 / miRNA

Outline of Final Research Achievements

Mismatch repair-deficient colorectal cancers display widespread instability at DNA microsatellite sequences (MSI). But MSI has been reported to commonly occur at coding repeats, leading to alterations in the function of a number of genes encoding cancer-related proteins, nothing is known about the putative impact of this process on non-coding microRNAs.
We considered whether genetic mutation due to MSI changed the structure of miRNA hairpin loop by using thermodynamic free energy.Our results provide evidence that DNA repeats contained in human miRNA gene are relatively rare and preserved from mutations due to MSI.

Research Products

• [Journal Article] Upregulated Annexin A1 promotes cellular invasion in triple-negative breast cancer. (2015)
  • Author(s): Okano M, Kumamoto K, Saito M, Onozawa H, Saito K, Abe N, Ohtake T, Takenoshita S.
  • Journal Title: Oncol Rep. Volume: 33(3) Issue: 3 Pages: 1064-70
  • DOI: 10.3892/or.2015.3720
  • Peer Reviewed / Open Access
• [Journal Article] Water intake disorder in a DEND syndrome afflicted patient with R50P mutation (2015)
  • Author(s): Maejima Y, Hasegawa S, Horita S, Kumamoto K, Galbanovskis J, Takenoshita S, Shimomura K.
  • Journal Title: Endocrine Journal Volume: 62 Issue: 4 Pages: 387-392
  • DOI: 10.1507/endocrj.EJ14-0392
  • NAID: 130005066941
  • ISSN: 0918-8959, 1348-4540
  • Peer Reviewed / Open Access
• [Journal Article] TACTILE SENSOR IS USEFUL FOR ESTIMATING LIVER HARDNESS AND LIVER FIBROSIS COMPARED WITH ULTRASONOGRAPHY AND COMPUTED TOMOGRAPHY (2014)
  • Author(s): atoshi Suzuki, Yohei Watanabe, Takashi Yazawa, Teruhide Ishigame, Motoki Sassa, Tomoyuki Monma, Tadashi Takawa, Kensuke Kumamoto, Izumi Nakamura, Shinji Ohoki, Yuichi Hatakeyama, Hiroshi Sakuma, Toshiyuki Ono, Sadao Omata, Seiichi Takenoshita
  • Journal Title: FUKUSHIMA JOURNAL OF MEDICAL SCIENCE Volume: 60 Issue: 2 Pages: 116-122
  • DOI: 10.5387/fms.2013-7
  • NAID: 130004697214
  • ISSN: 0016-2590, 2185-4610
  • Peer Reviewed / Open Access
• [Journal Article] A three-microRNA signature predicts responses to platinum-based doublet chemotherapy in patients with lung adenocarcinoma. (2014)
  • Author(s): Saito M, Shiraishi K, Matsumoto K, Schetter AJ, Ogata-Kawata H, Tsuchiya N, Kunitoh H, Nokihara H, Watanabe S, Tsuta K, Kumamoto K, Takenoshita S, Yokota J, Harris CC, Kohno T
  • Journal Title: Clin Cancer Res. Volume: 20(18) Pages: 4784-93
  • Peer Reviewed / Open Access
• [Journal Article] The expression of four genes as a prognostic classifier for stage I lung adenocarcinoma in 12 independent cohorts. (2014)
  • Author(s): Okayama H, Schetter AJ, Ishigame T, Robles AI, Kohno T, Yokota J, Takenoshita S, Harris CC.
  • Journal Title: Cancer Epidemiol Biomarkers Prev. Volume: 23(12) Pages: 2884-94
  • Peer Reviewed / Open Access
• [Journal Article] Increased IL-17 production correlates with immunosuppression involving myeloid-derived suppressor cells and nutritional impairment in patients with various gastrointestinal cancers. (2013)
  • Author(s): Yazawa T, Shibata M, Gonda K, Machida T, Suzuki S, Kenjo A, Nakamura I, Tsuchiya T, Koyama Y, Sakurai K, Shimura T, Tomita R, Ohto H, Gotoh M, Takenoshita S.
  • Journal Title: Molecular and Clinical Oncology Volume: 1 Issue: 4 Pages: 675-679
  • DOI: 10.3892/mco.2013.134
  • Peer Reviewed
• [Presentation] Three-Year Results and Future Scope of the Fukushima Thyroid Ultrasound Examination after the Fukushima Nuclear Power Plant Accident (2015)
  • Author(s): Shinichi Suzuki, Satoru Suzuki, Toshihiko Fukushima, Izumi Nakamura, Hiroki Shimura, Sanae Midorikawa, Tetsuya Ohira, and Shunichi Yamashita
  • Organizer: ENDO 2015
  • Place of Presentation: San Diego
  • Year and Date: 2015-03-07
• [Presentation] Systematic Determination of Thyroid Volume By Ultrasound Examination from the Infant to Adolescence in Japan: The Fukushima Health Management Survey (2015)
  • Author(s): Izumi Nakamura, Satoru Suzuki, Sanae Midorikawa, Toshihiko Fukushima, Hiroki Shimura, Tetsuya Ohira, Akira Ohtsuru, Masafumi Abe, Yoshisada Shibata, Satoshi Suzuki, Shunichi Yamashita and Shinichi Suzuki
  • Organizer: ENDO 2015
  • Place of Presentation: San Diego
  • Year and Date: 2015-03-06
• [Presentation] Preliminary Results in Prevalence of Thyroid Hemiagenesis and Hypoplasia: The Fukushima Health Management Survey (2015)
  • Author(s): Satoshi Suzuki, Satoru Suzuki, Sanae Midorikawa, Toshihiko Fukushima, Hiroki Shimura, Tetsuya Ohira, Akira Ohtsuru, Masafumi Abe, Izumi Nakamura, Shunichi Yamashita, and Shinichi Suzuki
  • Organizer: ENDO 2015
  • Place of Presentation: San Diego
  • Year and Date: 2015-03-05
• [Presentation] アミノ酸トランスポーターLAT1の臨床病理学的意義ならびに画像マーカーの開発 (2013)
  • Author(s): 織内 昇, 隈元 謙介, 中村 泉, 解良 恭一, 富永 英之, 竹之下 誠一
  • Organizer: 第51回日本癌治療学会
  • Place of Presentation: 京都