Discovery of predictive biomarkers for molecular target drug effectiveness of advanced colorectal cancer using phosphoproteomics

• Project/Area Number: 24659622
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Digestive surgery
• Research Institution: 独立行政法人医薬基盤研究所
• Principal Investigator: TOMONAGA Takeshi 独立行政法人医薬基盤研究所, 創薬基盤研究部, プロジェクトリーダー (80227644)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: リン酸化プロテオミクス / 分子標的薬 / バイオマーカー / 薬効予測 / 大腸癌 / 大腸がん

Research Abstract

For practice of personalized medicine, prediction of molecular target drug effectiveness and selection of sensitive patients to the drug are needed. Effect of drug depends on the activity of growth signals in which the drug is involved. Protein phosphorylation plays a crucial role for the activity of growth signals.
We aimed at establishment of comprehensive quantitation method for phosphoproteins and discovery of predictive biomarkers for molecular target drug effectiveness of advanced colorectal cancer using the method. We modified previous phosphoproteomics technologies and succeeded in identification of 30000 phosphopeptides in one experiment. This is the largest study of phosphoproteomics identification to date. We also showed that activity of PDPK1 kinase, a kinase downstream of EGFR signaling pathway, might be involved in sensitivity of an anti-EGFR antibody drug Cetuximab.

Research Products

• [Journal Article] Discovery of colorectal cancer biomarker candidates by membrane proteomic analysis and subsequent verification using selected reaction monitoring and tissue microarray analysis (2014)
  • Author(s): Kume H., Muraoka S., Kuga T., Adachi J., Narumi R., Watanabe S., Kuwano M., Kodera Y., Matsushita K., Fukuoka J., Masuda T., Ishihama Y., Matsubara H., Nomura F., and Tomonaga T
  • Journal Title: Mol Cell Proteomics Volume: (in press)
• [Journal Article] Lamin B2 prevents chromosome instability by ensuring proper mitotic chromosome segregation (2014)
  • Author(s): Kuga, T., Nie H., Kazami T., Satoh M., Matsushita K., Nomura F., Maeshima K., Nakayama Y., and Tomonaga T
  • Journal Title: Oncogenesis Volume: (in press)
• [Journal Article] Absolute quantitation of low abundance plasma APL1βpeptides at sub fmol/mL level by SRM/MRM without immunoaffinity enrichment (2014)
  • Author(s): Sano S., Tagami S., Hashimoto Y., Yoshizawa-Kumagaye K., Tsunemi M., Okochi M., and Tomonaga T
  • Journal Title: J Proteome Res Volume: (in press)
• [Journal Article] Selective evaluation of high density lipoprotein from mouse small intestines by an in situ perfusion technique (2014)
  • Author(s): Y amaguchi S., Zhang B., Tomonaga T, Seino U., Kanagawa A., Segawa M., Nagasaka H., Suzuki A., Miida T., Yamada S., Sasaguri Y., Doi T., Saku K., Okazaki M., Tochino Y., and Hirano K
  • Journal Title: J Lipid Res Volume: (in press)
• [Journal Article] Discovery of colorectal cancer biomarker candidates by membrane proteomic analysisand subsequent verification using selected reaction monitoring and tissue microarrayanalysis (2014)
  • Author(s): Kume H, Muraoka S, Kuga T, Adachi J, Narumi R, Watanabe S, Kuwano M, Kodera Y, Matsushita K, Fukuoka J, Masuda T, Ishihama Y, Matsubara H, Nomura F, Tomonaga T.
  • Journal Title: Mol Cell Proteomics. Volume: M113 Issue: 6 Pages: 37093-37093
  • DOI: 10.1074/mcp.m113.037093
  • Peer Reviewed
• [Journal Article] A novel mechanism of keratin cytoskeleton organization through casein kinase I alpha and FAM83H in colorectal cancer (2013)
  • Author(s): Kuga, T., Kume, H., Kawasaki, N., Sato, M., Adachi, J., Shiromizu, T., Hoshino, I., Nishimori, T., Matsubara, H., and Tomonaga T
  • Journal Title: J Cell Sci Volume: 126 Pages: 4721-31
• [Journal Article] Formation of long and winding nuclear F-actin bundles by nuclear c-Abl tyrosine kinase (2013)
  • Author(s): Aoyama K., Yuki R., Horiike Y., Kubota S., Yamaguchi N., Morii M., Ishibashi K., Nakayama Y., Kuga T., Hashimoto Y., Tomonaga T, and Yamaguchi N
  • Journal Title: Exp Cell Res Volume: 319 Pages: 3251-68
• [Journal Article] Phosphorylation of KRAB-associated Protein 1 (KAP1) at Tyr-449, Tyr-458, and Tyr-517 by Nuclear Tyrosine Kinases Inhibits the Association of KAP1 and Heterochromatin Protein 1alpha (HP1alpha) with Heterochromatin (2013)
  • Author(s): Kubota, S., Fukumoto, Y., Aoyama, K., Ishibashi, K., Yuki, R., Morinaga, T., Honda, T., Yamaguchi, N., Hashimoto, Y., Kuga, T., Tomonaga T, and Yamaguchi, N
  • Journal Title: J Biol Chem Volume: 288 Pages: 17871-17883
• [Journal Article] In-depth Membrane Proteomic Study of Breast Cancer Tissues for the Generation of a Chromosome-based Protein List (2013)
  • Author(s): Muraoka, S., Kume, H., Adachi, J., Shiromizu, T., Watanabe, S., Masuda, T., Ishihama, Y., and Tomonaga T
  • Journal Title: J Proteome Res Volume: 12 Pages: 208-213
• [Journal Article] Identification of Missing Proteins in the neXtProt Database and Unregistered Phosphopeptides in the PhosphoSitePlus Database As Part of the Chromosome-Centric Human Proteome Project (2013)
  • Author(s): Shiromizu, T., Adachi, J., Watanabe, S., Murakami, T., Kuga, T., Muraoka, S., and Tomonaga T
  • Journal Title: J Proteome Res Volume: 12 Pages: 2414-2421
• [Journal Article] Lamin B2 prevents chromosome instability by ensuring proper mitotic chromosome segregation (2013)
  • Author(s): Kuga, T., Nie, H., Kazami, T., Satoh, M., Matsushita, K., Nomura, F., Maeshima, K., Nakayama, Y., Tomonaga, T.
  • Journal Title: Oncogenesis Volume: 3 Issue: 3 Pages: e94-e94
  • DOI: 10.1038/oncsis.2014.6
  • Peer Reviewed
• [Journal Article] Absolute Quantitation of Low Abundance Plasma APL1β peptides at Sub-fmol/mL Level by SRM/MRM without Immunoaffinity Enrichment. (2013)
  • Author(s): Sano S, Tagami S, Hashimoto Y, Yoshizawa-Kumagaye K, Tsunemi M, Okochi M, Tomonaga T.
  • Journal Title: J Proteome Res. Volume: 13 Issue: 2 Pages: 10121020-10121020
  • DOI: 10.1021/pr4010103
  • Peer Reviewed
• [Journal Article] A novel mechanism of keratin cytoskeleton organization through casein kinase I alpha and FAM83H in colorectal cancer. (2013)
  • Author(s): Kuga, T., Kume, H., Kawasaki, N., Sato, M., Adachi, J., Shiromizu, T., Hoshino, I., Nishimori, T., Matsubara, H., and Tomonaga T.
  • Journal Title: J Cell Sci Volume: 126 Pages: 4721-31
  • DOI: 10.1242/jcs.129684
  • Peer Reviewed
• [Journal Article] Formation of long and winding nuclear F-actin bundles by nuclear c-Abl tyrosine kinase. (2013)
  • Author(s): Aoyama K., Yuki R., Horiike Y., Kubota S., Yamaguchi N., Morii M., Ishibashi K., Nakayama Y., Kuga T., Hashimoto Y., Tomonaga T, and Yamaguchi N.
  • Journal Title: Exp Cell Res Volume: 319 Issue: 20 Pages: 3251-68
  • DOI: 10.1016/j.yexcr.2013.09.003
  • Peer Reviewed
• [Journal Article] Phosphorylation of KRAB-associated Protein 1 (KAP1) at Tyr-449, Tyr-458, and Tyr-517 by Nuclear Tyrosine Kinases Inhibits the Association of KAP1 and Heterochromatin Protein 1alpha (HP1alpha) with Heterochromatin. (2013)
  • Author(s): Kubota, S., Fukumoto, Y., Aoyama, K., Ishibashi, K., Yuki, R., Morinaga, T., Honda, T., Yamaguchi, N., Hashimoto, Y., Kuga, T., Tomonaga T, and Yamaguchi, N.
  • Journal Title: J Biol Chem Volume: 288, Issue: 24 Pages: 17871-17883
  • DOI: 10.1074/jbc.m112.437756
  • Peer Reviewed
• [Journal Article] Identification of Missing Proteins in the neXtProt Database and Unregistered Phosphopeptides in the PhosphoSitePlus Database As Part of the Chromosome-Centric Human Proteome Project. (2013)
  • Author(s): Shiromizu T, Adachi J, Watanabe S, Murakami T, Kuga T, Muraoka S, Tomonaga T
  • Journal Title: J Proteome Res. Volume: in press
  • Peer Reviewed
• [Journal Article] A strategy for SRM-based verification of biomarker candidates discovered by iTRAQ method in limited breast cancer tissue samples (2012)
  • Author(s): Muraoka S, Kume H, Watanabe S, Adachi J, Kuwano M, Sato M, Kawasaki N, Kodera Y, Ishitobi M, Inaji H, Miyamoto Y, Kato K, Tomonaga T
  • Journal Title: J Proteome Res Volume: 11 Pages: 4201-4210
• [Journal Article] A Strategy for Large-Scale Phosphoproteomics and SRM-Based Validation of Human Breast Cancer Tissue Samples (2012)
  • Author(s): Narumi R, Murakami T, Kuga T, Adachi J, Shiromizu T, Muraoka S, Kume H, Kodera Y, Matsumoto M, Nakayama K, Miyamoto Y, Ishitobi M, Inaji H, Kato K, Tomonaga T
  • Journal Title: J Proteome Res Volume: 11 Pages: 5311-5322
• [Journal Article] A strategy for large-scale phosphoproteomics and SRM-based validation of human breast cancer tissue samples. (2012)
  • Author(s): Narumi R, Murakami T, Kuga T, Adachi J, Shiromizu T, Muraoka S, Kume H, Kodera Y, Matsumoto M, Nakayama K, Miyamoto Y, Ishitobi M, Inaji H, Kato K, Tomonaga T.
  • Journal Title: J Proteome Res. Volume: 11 Issue: 11 Pages: 5311-5322
  • DOI: 10.1021/pr3005474
  • Peer Reviewed
• [Presentation] 質量分析計を用いたバイオマーカー定量法の実用化 (2014)
  • Author(s): 朝長毅
  • Organizer: 第11回北里疾患プロテオミクス研究会
  • Place of Presentation: 神奈川
  • Year and Date: 2014-03-28
• [Presentation] 質量分析計を用いたバイオマーカー定量法の実用化 (2014)
  • Author(s): 朝長 毅
  • Organizer: 第11 回 北里疾患プロテオミクス研究会
  • Place of Presentation: 神奈川
  • Invited
• [Presentation] 質量分析計を用いたバイオマーカー定量法の実用化への挑戦 (2013)
  • Author(s): 朝長毅
  • Organizer: 第10回千葉疾患プロテオミクス研究会
  • Place of Presentation: 東京
  • Year and Date: 2013-11-09
• [Presentation] ターゲットプロテオミクス～Beyond SRM～ (2013)
  • Author(s): 足立淳、朝長毅
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2013-09-13
• [Presentation] 自己抗体ファージライブラリを用いたスキルス胃癌の診断バイオマーカーの探索 (2013)
  • Author(s): 村岡賢,久米秀明,西塚哲,若林剛,星野敢,松原久裕,朝長毅
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] マウス同所移植モデルによる大腸癌高浸潤性細胞のプロテオーム解析 (2013)
  • Author(s): 白水崇、足立淳、八木滋雄、朝長毅
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] 大腸癌細胞におけるFAM83Hとcasein kinase Iαを介したケラチン骨格制御機構の解明 (2013)
  • Author(s): 久家貴寿,久米秀明,川崎直子,足立淳,星野敢,松原久裕,齊藤洋平,中山祐治,朝長毅
  • Organizer: 第36回日本分子生物学会
  • Place of Presentation: 神戸
• [Presentation] ATP Accessibility Screening (AAS), A High-Throughput and High-Resolution Kinase Analysis Platform for Signaling Research (2013)
  • Author(s): Adachi J, Higo D, Watanabe S, Kuwano M, Hashimoto Y, Tomonaga T
  • Organizer: Human Proteome Organization (HUPO) 12^<th> Annual World Congress
  • Place of Presentation: Yokohama, Japan
• [Presentation] Discovery and subsequent validation of biomarkers for colorectal cancer by large-scale proteomic analysis and tissue microarray analysis (2013)
  • Author(s): Kume H, Muraoka S, Hashimoto Y, Watanabe S, Masuda T, Ishihama Y, Fukuoka J, Kodera Y, Matsushita K, Matsubara H, Tomonaga T
  • Organizer: Human Proteome Organization (HUPO) 12^<th> Annual World Congress
  • Place of Presentation: Yokohama, Japan
• [Presentation] Proteomic Analysis of Highly Invasive Colorectal Cancer Cells Established by Orthotopic Xenograft Mouse Model (2013)
  • Author(s): Shiromizu T, Adachi J, Yagi S, Hoffman RM, Tomonaga T
  • Organizer: Human Proteome Organization (HUPO) 12^<th> Annual World Congress
  • Place of Presentation: Yokohama, Japan
• [Presentation] Development of High-throughput Screening System Using Autoantibody Library for Discovery of Scirrhous Gastric Cancer Biomarker (2013)
  • Author(s): Muraoka S, Kume H, Saitoh H, Enomoto Y, Ito Y, Nishizuka S, Wakabayashi G, Hoshino I, Matsubara H, and Tomonaga T
  • Organizer: Human Proteome Organization (HUPO) 12^<th> Annual World Congress
  • Place of Presentation: Yokohama, Japan
• [Presentation] Quantitative Phosphoproteome Analysis of Cultured Stomach Cancer Cell Lines Aimed at Development of Biomarkers for Prediction of Drug Efficacy (2013)
  • Author(s): Hashiguchi K, Muraoka S, Adachi J, Sato M, Kuga T, Watanabe R, Shiromizu T, Hashimoto Y, Nagano M, Kishida M, Tomonaga T
  • Organizer: Human Proteome Organization (HUPO) 12^<th> annual world congress
  • Place of Presentation: Yokohama, Japan
• [Presentation] Quantitative Proteome Analysis with Isotope Dimethyl Labeling to Identify TGF-beta-mediated Tumor Proteins Using the Metastatic Mouse Breast Cancer Model (2013)
  • Author(s): Sato M, Adachi J, Tomonaga T
  • Organizer: Human Proteome Organization (HUPO) 12^<th> Annual World Congress
  • Place of Presentation: Yokohama, Japan
• [Presentation] Phospho-profiling of mTOR inhibitor-treated renal cell carcinoma (RCC) cell lines and its application for drug response-efficacy biomarkers (2013)
  • Author(s): Watanabe R, Hashimoto Y, Kishida M, Matsubara M, Adachi J, Tomonaga T
  • Organizer: Human Proteome Organization (HUPO) 12^<th> Annual World Congress
  • Place of Presentation: Yokohama, Japan
• [Presentation] A Novel Mechanism of Keratin Cytoskeleton Organization Through Casein Kinase I Alpha and FAM83H in Colorectal Cancer : Interactome Analysis of FAM83H (2013)
  • Author(s): Kuga T, Kume H, Kawasaki N, Sato M, Adachi J, Shiromizu T, Hoshino I, Matsubara H, Tomonaga T
  • Organizer: Human Proteome Organization (HUPO) 12^<th> Annual World Congress
  • Place of Presentation: Yokohama, Japan
• [Presentation] Absolute quantitation of plasma biomarker peptides APL1b for Alzheimer disease at fmol/ml level using SRM (2013)
  • Author(s): Sano S, Tagami S, Yoshizawa-Kumagaye K, Tsunemi M, Okochi M, Tomonaga T
  • Organizer: Human Proteome Organization (HUPO) 12^<th> Annual World Congress
  • Place of Presentation: Yokohama, Japan
• [Presentation] A Kinase Activity-Estimating Method Using LC-MS/MS (2013)
  • Author(s): Nagano M, Kuga T, Adachi J, Tomonaga T
  • Organizer: Human Proteome Organization (HUPO) 12^<th> Annual World Congress
  • Place of Presentation: Yokohama, Japan
• [Presentation] 質量分析計を用いたバイオマーカー定量法の実用化への挑戦 (2013)
  • Author(s): 朝長 毅
  • Organizer: 第10 回 千葉疾患プロテオミクス研究会
  • Place of Presentation: 東京
  • Invited
• [Presentation] ATP Accessibility Screening (AAS), A High-Throughput and High-Resolution Kinase Analysis Platform for Signaling Research. Human Proteome Organization (2013)
  • Author(s): Adachi J, Higo D, Watanabe S, Kuwano M, Hashimoto Y, Tomonaga T.
  • Organizer: (HUPO) 12th Annual World Congress
  • Place of Presentation: 横浜
  • Invited
• [Presentation] Discovery and subsequent validation of biomarkers for colorectal cancer by large-scale proteomic analysis and tissue microarray analysis. (2013)
  • Author(s): Kume H, Muraoka S, Hashimoto Y, Watanabe S, Masuda T, Ishihama Y, Fukuoka J, Kodera Y, Matsushita K, Matsubara H, Tomonaga T.
  • Organizer: Human Proteome Organization (HUPO) 12th annual world congress
  • Place of Presentation: 横浜
• [Presentation] Proteomic Analysis of Highly Invasive Colorectal Cancer Cells Established byOrthotopic Xenograft Mouse Model. Human Proteome Organization (2013)
  • Author(s): Shiromizu T, Adachi J, Yagi S, Hoffman RM, Tomonaga T.
  • Organizer: (HUPO) 12th Annual World Congress
  • Place of Presentation: 横浜
• [Presentation] Development of High-throughput Screening System Using Autoantibody Library for Discovery of Scirrhous Gastric Cancer Biomarker. Human Proteome Organization (2013)
  • Author(s): Muraoka S, Kume H, Saitoh H, Enomoto Y, Ito Y, Nishizuka S, Wakabayashi G, Hoshino I, Matsubara H, and Tomonaga T.
  • Organizer: (HUPO) 12th Annual World Congress
  • Place of Presentation: 横浜
• [Presentation] Quantitative Phosphoproteome Analysis of Cultured Stomach Cancer Cell Lines Aimed at Development of Biomarkers for Prediction of Drug Efficacy. (2013)
  • Author(s): Hashiguchi K, Muraoka S, Adachi J, Sato M, Kuga T, Watanabe R, Shiromizu T, Hashimoto Y, Nagano M, Kishida M, Tomonaga T.
  • Organizer: Human Proteome Organization (HUPO) 12th annual world congress
  • Place of Presentation: 横浜
• [Presentation] Quantitative Proteome Analysis with Isotope Dimethyl Labeling to Identify TGF-beta-mediated Tumor Proteins Using the Metastatic Mouse Breast Cancer Model. (2013)
  • Author(s): Sato M, Adachi J, Tomonaga T.
  • Organizer: Human Proteome Organization (HUPO) 12th annual world congress
  • Place of Presentation: 横浜
• [Presentation] Phospho-profiling of mTOR inhibitor-treated renal cell carcinoma (RCC) cell lines and its application for drug response-efficacy biomarkers. (2013)
  • Author(s): Watanabe R, Hashimoto Y, Kishida M, Matsubara M, Adachi J, Tomonaga T.
  • Organizer: Human Proteome Organization (HUPO) 12th annual world congress
  • Place of Presentation: 横浜
• [Presentation] A Novel Mechanism of Keratin Cytoskeleton Organization Through Casein Kinase I Alpha and FAM83H in Colorectal Cancer: Interactome Analysis of FAM83H. (2013)
  • Author(s): Kuga T, Kume H, Kawasaki N, Sato M, Adachi J, Shiromizu T, Hoshino I, Matsubara H, Tomonaga T.
  • Organizer: Human Proteome Organization (HUPO) 12th annual world congress
  • Place of Presentation: 横浜
• [Presentation] A Kinase Activity-Estimating Method Using LC-MS/MS. (2013)
  • Author(s): Nagano M, Kuga T, Adachi J, Tomonaga T.
  • Organizer: Human Proteome Organization (HUPO) 12th annual world congress
  • Place of Presentation: 横浜
• [Presentation] プロテオミクスを用いた新規腫瘍マーカーの探索と実用化 (2012)
  • Author(s): 朝長毅
  • Organizer: 第32回日本分子腫瘍マーカー研究会
  • Place of Presentation: 札幌
  • Year and Date: 2012-09-18
• [Presentation] 最近のプロテオミクス技術の進歩とがん研究への応用 (2012)
  • Author(s): 朝長毅
  • Organizer: 第112回日本外科学会定期学術集会
  • Place of Presentation: 千葉
  • Year and Date: 2012-04-14
• [Presentation] 真のバイオマーカーの発見を目指して (2012)
  • Author(s): 朝長毅
  • Organizer: 第10回日本プロテオーム学会2012年会
  • Place of Presentation: 東京
• [Presentation] 疾患プロテオミクスの基礎とHuman Proteome Project (2012)
  • Author(s): 朝長毅
  • Organizer: 第19回日本遺伝子診療学会
  • Place of Presentation: 千葉
• [Presentation] 大規模プロテオーム解析による大腸癌バイオマーカーの探索とその検証 (2012)
  • Author(s): 久米秀明,村岡賢,小寺義男,松下一之,松原久裕,朝長毅
  • Organizer: 第71回日本癌学会
  • Place of Presentation: 札幌
• [Presentation] A strategy for validation of biomarker candidates combining iTRAQ and SRM/MRM assay in breast cancer tissue samples (2012)
  • Author(s): 村岡賢, 久米秀明, 足立淳, 宮本泰豪,加藤菊也,小寺義男,朝長毅
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] オミックス技術を駆使した新規大腸癌関連タンパク質の同定 (2012)
  • Author(s): 久家貴寿, 久米秀明, 足立淳, 星野敢, 松原久裕, 朝長毅
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] リン酸化プロテオミクスを用いた新規DNA損傷初期応答キナーゼの探索 (2012)
  • Author(s): 足立淳, 久家貴寿, 白水崇, 久米秀明, 村岡賢, 中山敬一, 井倉毅, 高田穣, 朝長毅
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] ヒト乳がん組織の大規模リン酸化プロテオーム解析とSRMをベースにした検証法 (2012)
  • Author(s): 村上達夫, 久家貴寿, 足立淳, 白水崇, 中山敬一, 宮本泰豪, 加藤菊也, 小寺義男, 朝長毅
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] Proteomic analysis of highly metastatic colorectal cancer cells established from orthotopic metastatic mouse model (2012)
  • Author(s): 白水崇,足立淳,朝長毅
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 北海道
• [Presentation] 細気管支肺胞上皮癌のプロテオーム解析によるバイオマーカー探索 (2012)
  • Author(s): 原康洋, 宮本泰豪, 加藤菊也, 福岡順也, 朝長毅
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] Phosphorylation dynamics in an early response of DNA damage signaling (2012)
  • Author(s): Adachi J, Kuga T, Shiromizu T, Kume H, Muraoka S, Hashiguchi K, Narumi R, Watanabe S, Kuwano M, Matsumoto M, Nakayama KI, Ikura M, Ikura T, Takata M Tomonaga T
  • Organizer: HUPO2012 11^<th> World Congress
  • Place of Presentation: Boston, U.S.A.
• [Presentation] A strategy for SRM-based systematic validation of biomarker candidates discovered by iTRAQ method in breast cancer tissue samples (2012)
  • Author(s): Muraoka S, Kume H, Watanabe S, Kuwano M, Sato M, Kawasaki N, Adachi J, Ishitobi M, Inaji H, Miyamoto Y, Kato K, Kodera Y, Tomonaga T
  • Organizer: HUPO2012 11^<th> World Congress
  • Place of Presentation: Boston, USA
• [Presentation] Quantitative proteomic profiling of orthotopic xenograft mouse model of colorectal cancer metastasi (2012)
  • Author(s): Shiromizu T, Adachi J, Tomonaga T
  • Organizer: HUPO2012 11^<th> World Congress
  • Place of Presentation: Boston, USA
• [Presentation] ATP Accessibility Screening (AAS), a high-throughput and high-resolution kinase analysis platform for signaling research (2012)
  • Author(s): Adachi J, Higo D, Watanabe S, Kuwano M, Hashimoto Y Tomonaga T
  • Organizer: 2^<nd> Copenhagen Bioscience Conference
  • Place of Presentation: Copenhagen, Denmark
• [Presentation] 疾患プロテオミクスの基礎とHuman Proteome Project (2012)
  • Author(s): 朝長 毅
  • Organizer: 第19回日本遺伝子診療学会2012年7月26-28
  • Place of Presentation: 千葉
  • Invited
• [Presentation] 大規模リン酸化プロテオーム解析とSRM/MRMによるヒト乳癌組織の検証法 (2012)
  • Author(s): 村上達夫, 久家貴寿, 足立淳, 白水崇, 宮本泰豪, 加藤菊也, 石飛真人, 稲治英生, 小寺義男, 朝長毅
  • Organizer: 第10回日本プロテオーム学会2012年会
  • Place of Presentation: 東京
• [Remarks]
  • URL: http://www.nibio.go.jp/proteome/index.html
• [Remarks] 独立行政法人医薬基盤研究所 プロテオームリサーチプロジェクト
  • URL: http://www.nibio.go.jp/proteome/index.html
• [Patent(Industrial Property Rights)] 乳がん治療の予後判定方法 (2012)
  • Inventor(s): 朝長 毅、村岡 賢、村上達夫、加藤菊也、宮本泰豪
  • Industrial Property Rights Holder: 朝長 毅、村岡 賢、村上達夫、加藤菊也、宮本泰豪
  • Industrial Property Rights Type: 特許
  • Filing Date: 2012-05-23