Novel tracheal reconstruction using iPS cells of Beagle dogs.

• Project/Area Number: 24659628
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Thoracic surgery
• Research Institution: Kyoto University
• Principal Investigator: NAKAMURA Tatsuo 京都大学, 再生医科学研究所, 准教授 (70227908)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 気管 / 再建 / 幹細胞 / iPS細胞 / ビーグル犬 / 気道 / 人工気管 / 組織工学 / 気道再生 / 脂肪由来幹細胞 / 気管狭窄 / 自己組織化 / 気道再建 / コラーゲン / 再生医療 / 胸部外科学

Research Abstract

We challenged to establish new iPS cell line from adult Beagle dogs, but it was much difficult than we had expected before the beginning of this experiment, to reset the mature cells of Beagle dogs. Hence we could not establish the cell line within the study period. However it was revealed the potential of ADS cells or bone mallow cell to the cell source of in situ Tissue Engineering instead of iPS cells. This method will contribute the reconstruction of airway as a new surgical approach. The reason may be due to the difference of the aging of the cell source, namely the cell from adult Beagle dog may have an unknown mechanism which works against the reprograming.

Research Products

• [Journal Article] Evaluation of the Use of Induced Pluripotent Stem Cells (iPSCs) for the Regeneration of Tracheal Cartilage (2013)
  • Author(s): Imaizumi M, Omori K, et al.
  • Journal Title: Cell Transplantation Volume: 22 Issue: 2 Pages: 341-353
  • DOI: 10.3727/096368912x653147
  • Peer Reviewed
• [Journal Article] Novel tracheal prosthesis using in situ Tissue Engineering (2013)
  • Author(s): Nakamura, T., Kojima, F., Sato, T., Hamaji, M., Kaneko, M., Kanemaru, S., Nakada, A., Omori, K., Shigeno, K., Wakatsuki, M., Endo, K
  • Journal Title: Int J Artif Organs Volume: 36 Pages: 585-585
  • Peer Reviewed
• [Journal Article] Regeneration of Canine Inferier Alveolar Nerve by Polyglycolic Acid-Collagen Tube with Platelet Rich Plasma (2013)
  • Author(s): Shigeno, K., Kaneko, M., Wakatsuki, M., Nakada, A., Hashimoto, Y., Honda, M., Inada, Y., Nakamura, T
  • Journal Title: Int J Artif Organs Volume: 36 Pages: 571-571
  • Peer Reviewed
• [Journal Article] Manufacture of a weakly denatured collagen fiber scaffold with excellent biocompatibility and space maintenance ability. (2013)
  • Author(s): Nakada, A., Shigeno, K., Sato, T., Kobayashi, T., Wakatsuki, M., Uji, M., Nakamura, T.
  • Journal Title: Biomed Meter. Volume: 8 Issue: 4 Pages: 45010-45019
  • DOI: 10.1088/1748-6041/8/4/045010
  • Peer Reviewed
• [Journal Article] Cellular interactions via conditioned media induce in vivo nephron generation from tubular epithelial cells or mesenchymal stem cells. (2013)
  • Author(s): Machiguchi, T., Nakamura, T.
  • Journal Title: Biochemical and Biophysical Research Communications. Volume: 435 Issue: 3 Pages: 327-333
  • DOI: 10.1016/j.bbrc.2013.04.050
  • Peer Reviewed
• [Journal Article] 生体内組織再生誘導型の人工気管を用いた気道再建 (2013)
  • Author(s): 大森孝一、多田靖宏、野本幸男、谷亜希子、金丸眞一、中村達雄
  • Journal Title: 再生医療 Volume: 12 Pages: 57-63
• [Journal Article] Bioengineered trachea using autologous chondrocytes for regeneration of tracheal cartilage in a rabbit model (2013)
  • Author(s): Nomoto, M., Nomoto, Y., Tada, Y., Tani, A., Otsuki, K., Suzuki, R., Nakamura, T., Omori, K
  • Journal Title: Laryngoscope Volume: 123 Issue: 9 Pages: 2195-2201
  • DOI: 10.1002/lary.23784
  • Peer Reviewed
• [Journal Article] Novel tracheal prosthesis using in situ Tissue Engineering. (2013)
  • Author(s): Nakamura, T., Kojima, F., Sato, T., Hamaji, M., Kaneko, M., Kanemaru, S., Nakada, A., Omori, K., Shigeno, K., Wakatsuki, M., Endo, K.
  • Journal Title: Int J Artif Organs Volume: 36 Pages: 585-585
  • Peer Reviewed
• [Journal Article] Regeneration of Canine Inferier Alveolar Nerve by Polyglycolic Acid-Collagen Tube with Platelet Rich Plasma. (2013)
  • Author(s): Shigeno, K., Kaneko, M., Wakatsuki, M., Nakada, A., Hashimoto, Y., Honda, M., Inada, Y., Nakamura, T.
  • Journal Title: Int J Artif Organs. Volume: 36 Pages: 571-571
  • Peer Reviewed
• [Journal Article] 生体内組織再生誘導型の人工気管を用いた気道再建. (2013)
  • Author(s): 大森孝一、多田靖宏、野本幸男、谷亜希子、金丸眞一、中村達雄
  • Journal Title: 再生医療. Volume: 12 Pages: 57-63
• [Journal Article] Artificial trachea and In situ tissue engineering : twelve gean-follow up.in canine model (2012)
  • Author(s): Nakamura, T., Fumitsugu, K., Sato, T., Nakada, A., Wakatsuki, M., Sasauchi, K., Kida, N., Kanemaru, S., Omori, K., Kaneko, M., Shigeno, k., Inada, Y., Endo, K
  • Journal Title: Int Artif Organs Volume: 35(8) Pages: 608-608
  • Peer Reviewed
• [Journal Article] 肺に対する再生医療応用の可能性 (2012)
  • Author(s): 中村達雄
  • Journal Title: 侵襲と免疫 Volume: 21(2) Pages: 7-12
• [Journal Article] Accelerated generation of human induced pluripotent stem cells with retroviral transduction and chemical inhibitors under physiological hypoxia (2012)
  • Author(s): Shimada, H., Hashimoto, Y., Nakada, A., Shigeno, K., Nakamura, T.
  • Journal Title: BBRC Volume: 417 Issue: 2 Pages: 659-664
  • DOI: 10.1016/j.bbrc.2011.11.111
  • Peer Reviewed
• [Journal Article] Fluid overload deteriorate the chylothorax (2012)
  • Author(s): Honda, M.
  • Journal Title: Diseases of the Esophagus Volume: 25 Issue: 3 Pages: 269-272
  • DOI: 10.1111/j.1442-2050.2011.01237.x
  • Peer Reviewed
• [Journal Article] Transforming Growth Factor/33 for the prevention of vocal fold scarring (2012)
  • Author(s): Ohno, S., Hirano, S., Kanemaru, S., Kitani, Y., Kojima, T., Ishikawa, S., Mizuta, M., Tateya, I., Nakamura, T., Ito, J.
  • Journal Title: Laryngoscope Volume: 122 Issue: 3 Pages: 583-589
  • DOI: 10.1002/lary.22389
  • Peer Reviewed
• [Journal Article] PGAチューブによる反回神経再建を施行した甲状腺癌長期経過の一症例 (2012)
  • Author(s): 平野滋、金丸眞一、中村達雄、伊藤壽一
  • Journal Title: 頭頸部癌 Volume: 38(3) Pages: 363-367
  • NAID: 10031170538
• [Journal Article] in situ Tissue Engineeringによる人工食道の開発 (2012)
  • Author(s): 中瀬有遠、萩原明於、中村達雄
  • Journal Title: G.I. Research Volume: 20(5) Pages: 40-43
• [Journal Article] Intrahepatic biliary ablation with pure ethanol: an experimental model of biliary atresia (2012)
  • Author(s): Yukihiro Tatekawa. Akira Nakada. Tatsuo Nakamura
  • Journal Title: Surg Today Volume: in press Issue: 6 Pages: 661-669
  • DOI: 10.1007/s00595-012-0379-2
  • NAID: 10031176089
  • Peer Reviewed
• [Journal Article] Regeneration of canine inferior alveolar nerve by polyglycolic acid-collagen tube (2012)
  • Author(s): Shigeno, k., Nakada, A., Kaneko, M., Wakatsuki, M., Hashimoto, Y., Inada, Y., Nakamura, T
  • Journal Title: Int Artif Organs Volume: 35(8) Pages: 569-569
  • Peer Reviewed
• [Journal Article] Promotion of bone repairing by use of novel Collagen scaffold in Rabbit skull defect model (2012)
  • Author(s): Wakatsuki, M., Kaneko, M., Sasauchi, K., Nakada, A., Shigeno, k., Nakamura, T
  • Journal Title: Int Artif Organs Volume: 35(8) Pages: 591-591
  • Peer Reviewed
• [Journal Article] The experiment of the bone regeneration in the canine fronfale (2012)
  • Author(s): Kaneko, M., Wakatsuki, M., Shigeno, k., Nakada, A. Nakamura, T
  • Journal Title: Int Artif Organs Volume: 35(8) Pages: 608-608
  • Peer Reviewed
• [Journal Article] Role of Circulating Mesenchymal Stem Cells in Vocal Fold Wound Healing (2012)
  • Author(s): Ohno, S., Hirano, S., Kanemaru, S., Mizuta, M., Ishikawa, S., Tateya, I., Nakamura, T., Ito, J
  • Journal Title: Laryngoscope Volume: 122(11) Issue: 11 Pages: 2503-2510
  • DOI: 10.1002/lary.23543
  • Peer Reviewed
• [Journal Article] Increased expression of phosphatidylcholine (16:0/18:1) and (16:0/18:2) in thyroid papillary cancer (2012)
  • Author(s): Ishikawa, S., Tateya, I., Hayakawa, T., Masaki, N., Takizawa, Y., Ohno, S., Kojima, T., Kitani, Y., Kitamura, M., Hirano, S., Setou, M., Ito, J
  • Journal Title: PLoS One Volume: 7(11) Issue: 11 Pages: e48873-e48873
  • DOI: 10.1371/journal.pone.0048873
  • Peer Reviewed
• [Journal Article] Regeneration of Tracheal Epithelium Using a Collagen Vitrigel-Sponge Scaffold Containing Basic Fibroblast Growth Factor (2012)
  • Author(s): Tani A, Tada Y, Takezawa T, Imaizumi M, Nomoto Y, Nakamura T, Omori K
  • Journal Title: Annals of Otology, Rhinology & Laryngology Volume: 121(4) Pages: 261-268
  • Peer Reviewed
• [Journal Article] 声門・声門下・気管狭窄の外来治療 : 喉頭気管溝形成と気道再建を中心に (2012)
  • Author(s): 大森孝一, 多田靖宏, 野本幸男, 谷亜希子, 金丸眞一, 中村達雄
  • Journal Title: 日本気管食道科学会会報 Volume: 63(2) Pages: 124-129
• [Journal Article] 消化器系における神経再生 : 神経再生を用いる直腸癌の新しい手術戦略 (2012)
  • Author(s): 萩原明於、辻本洋行、阪倉長平、中村達雄
  • Journal Title: G.I. Research Volume: 20(6) Pages: 48-55
• [Journal Article] Artificial trachea and In situ tissue engineering:twelve gean-follow up.in canine model. (2012)
  • Author(s): Nakamura, T.
  • Journal Title: Int Artif Organs. Volume: 35(8) Pages: 608-608
  • Peer Reviewed
• [Journal Article] 肺に対する再生医療応用の可能性. (2012)
  • Author(s): 中村達雄
  • Journal Title: 侵襲と免疫. Volume: 21 Pages: 7-12
• [Journal Article] PGAチューブによる反回神経再建を施行した甲状腺癌長期経過の一症例. (2012)
  • Author(s): 平野滋
  • Journal Title: 頭頸部癌. Volume: 38(3) Pages: 363-367
• [Journal Article] in situ Tissue Engineeringによる人工食道の開発. (2012)
  • Author(s): 中瀬有遠
  • Journal Title: G.I. Research. Volume: 20(5) Pages: 40-43
• [Journal Article] Regeneration of canine inferior alveolar nerve by polyglycolic acid-collagen tube. (2012)
  • Author(s): Shigeno, K.
  • Journal Title: Int Artif Organs. Volume: 35(8) Pages: 569-569
  • Peer Reviewed
• [Journal Article] Promotion of bone repairing by use of novel Collagen scaffold in Rabbit skull defect model. (2012)
  • Author(s): Wakatsuki, M.
  • Journal Title: Int Artif Organs. Volume: 35(8) Pages: 591-591
  • Peer Reviewed
• [Journal Article] The experiment of the bone regeneration in the canine fronfale. (2012)
  • Author(s): Kaneko, M.
  • Journal Title: Int Artif Organs. Volume: 35(8) Pages: 608-608
  • Peer Reviewed
• [Journal Article] Regeneration of Tracheal Epithelium Using a Collagen Vitrigel-Sponge Scaffold Containing Basic Fibroblast Growth Factor. (2012)
  • Author(s): Tani A
  • Journal Title: Annals of Otology, Rhinology & Laryngology Volume: 121(4) Pages: 261-268
  • Peer Reviewed
• [Journal Article] 声門・声門下・気管狭窄の外来治療：喉頭気管溝形成と気道再建を中心に. (2012)
  • Author(s): 大森孝一
  • Journal Title: 日本気管食道科学会会報 Volume: 63(2) Pages: 124-129
• [Journal Article] 消化器系における神経再生：神経再生を用いる直腸癌の新しい手術戦略. (2012)
  • Author(s): 萩原明於
  • Journal Title: G.I. Research. Volume: 20(6) Pages: 48-55
• [Presentation] In-Vivo Localization of Micro Radio Frequency Identification Tag : Concept Proof of Novel Surgical Marking System for Small Peripheral Lung Nodule (2013)
  • Author(s): Kojima, F., Sato, T., Takahata, H., Okada, M., Sugiura, T., Oshiro, O., Date, H., Nakamura. T
  • Organizer: 第21回アジア胸部心臓血管外科学会
  • Place of Presentation: 神戸
  • Year and Date: 2013-04-06
• [Presentation] Novel tracheal prosthesis using in situ Tissue Engineering (2013)
  • Author(s): Nakamura, T., Kojima, F., Sato, T., Hamaji, M., Kaneko, M., Kanemaru, S., Nakada, A., Omori, K., Shigeno, K., Wakatsuki, M., Endo, K
  • Organizer: 40th Annual ESAO Congress
  • Place of Presentation: Scotland
• [Presentation] Impact of Transmission Power Variation to the Position Estimation Error Performance of RFID-tag Assisted Surgery Support System (2013)
  • Author(s): Takahata, H., Sugiura, T., Kojima, F., Sato, T., Okada, M., Oshiro, O
  • Organizer: International Conference of the IEEE Engineering in Medicine and Biology Society(EMBC'2013)
  • Place of Presentation: Osaka
• [Presentation] Novel tracheal prosthesis using in situ Tissue Engineering. (2013)
  • Author(s): Nakamura, T., Kojima, F., Sato, T., Hamaji, M., Kaneko, M., Kanemaru, S., Nakada, A., Omori, K., Shigeno, K., Wakatsuki, M., Endo, K.
  • Organizer: 40th Annual ESAO Congress
  • Place of Presentation: Scotland (UK)
• [Presentation] Impact of Transmission Power Variation to the Position Estimation Error Performance of RFID-tag Assisted Surgery Support System． (2013)
  • Author(s): Takahata, H., Sugiura, T., Kojima, F., Sato, T., Okada, M., Oshiro, O.
  • Organizer: International Conference of the IEEE Engineering in Medicine and Biology Society(EMBC'2013)
  • Place of Presentation: 大阪
• [Presentation] 人工神経管(PGA-tube)による末梢神経再生とin situ Tissue Engineering(生体内再生) (2012)
  • Author(s): 中村達雄
  • Organizer: 第32回日本臨床麻酔学会
  • Place of Presentation: 福島
  • Year and Date: 2012-11-01
  • Invited
• [Presentation] In situ Tissue Engineering (2012)
  • Author(s): Nakamura, T
  • Organizer: Annual Meeting of The Korean Bronchoesophagological Society
  • Place of Presentation: Seoul(Korea)
  • Year and Date: 2012-05-12
  • Invited
• [Presentation] Clinical Application of a Tissue-engineered Trachea (2012)
  • Author(s): Nakamura, T
  • Organizer: Annual Meeting of The Korean Bronchoesophagological Society
  • Place of Presentation: Seoul(Korea)(Symposium)
  • Year and Date: 2012-05-12
• [Presentation] Role of Circulating Mesenchymal Stem Cells in Vocal Fold Wound Healing (2012)
  • Author(s): Ohno, S., Hirano, S., Kanemaru, S., Mizuta, M., Ishikawa, S., Tateya, I., Nakamura, T., Ito, J
  • Organizer: The 92nd Annual Meeting of The American Broncho-Esophagological Association
  • Place of Presentation: San Diego (USA)
  • Year and Date: 2012-04-19
• [Presentation] REGENERATION OF CANINE INFERIER ALVEOLAR NERVE BY POLGLY-COLIC ACID-COLLAGEN TUBE (2012)
  • Author(s): Shigeno, K., Shionoya, Y., Nakada, A., Kaneko, M., Wakatsuki, M., Hashimoto, Y., Inada, Y., Nakamura, T
  • Organizer: 39th Annual ESAO Congress
  • Place of Presentation: Rostock (Germany)
• [Presentation] 弱変性collagen fiber scaffoldの有用性について (2012)
  • Author(s): 中田顕
  • Organizer: 日本再生医療学会
  • Place of Presentation: 横浜
• [Presentation] REGENERATION OF CANINE INFERIER ALVEOLAR NERVE BY POLGLY-COLIC ACID-COLLAGEN TUBE. (2012)
  • Author(s): Shigeno, K.
  • Organizer: 39th Annual ESAO Congress
  • Place of Presentation: Rostock (Germany)
• [Presentation] 弱変性collagen fiber scaffoldの有用性について. (2012)
  • Author(s): 中田顕
  • Organizer: 日本再生医療学会
  • Place of Presentation: 横浜
• [Presentation] In-Vivo Localization of Micro Radio Frequency Identification Tag: Concept Proof of Novel Surgical Marking System for Small Peripheral Lung Nodule.
  • Author(s): Kojima, F., Sato, T., Takahata, H., Okada, M., Sugiura, T., Oshiro, O., Date, H., Nakamura. T.
  • Organizer: 第２１回アジア胸部心臓血管外科学会
  • Place of Presentation: 神戸
• [Presentation] In situ Tissue Engineering.
  • Author(s): Nakamura, T.
  • Organizer: Annual Meeting of The Korean Bronchoesophagological Society
  • Place of Presentation: Seoul(Korea)
  • Invited
• [Presentation] Clinical Application of a Tissue-engineered Trachea.
  • Author(s): Nakamura, T.
  • Organizer: Annual Meeting of The Korean Bronchoesophagological Society
  • Place of Presentation: Seoul(Korea)
• [Presentation] 人工神経管（PGA-tube）による末梢神経再生とin situ Tissue Engineering（生体内再生）．
  • Author(s): 中村達雄
  • Organizer: 第32回日本臨床麻酔学会
  • Place of Presentation: 福島
  • Invited
• [Presentation] Role of Circulating Mesenchymal Stem Cells in Vocal Fold Wound Healing.
  • Author(s): Ohno, S.
  • Organizer: The 92nd Annual Meeting of The American Broncho-Esophagological Association.
  • Place of Presentation: San Diego (USA)
• [Remarks]
  • URL: http://www.frontier.kyoto-u.ac.jp/ca04/index.html
• [Remarks] 京都大学再生医科学研究所 臓器再建応用分野 ホームページ
  • URL: http://www.frontier.kyoto-u.ac.jp/ca04/